Home » ROBO2 » ROBO2 Antibodies » ROBO2 Antibody [Unconjugated]
Novus Biologicals products are now on bio-techne.com

ROBO2 Antibody [Unconjugated]

 (10 Publications)
 See All Related
Submit a Review

Images

 
There are currently no images for ROBO2 Antibody (AF3147).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF3147
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF3147-SP
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

ROBO2 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human ROBO2
Ser22-Ala312, RPVA, Pro313-Pro859
Accession # Q9HCK4
Specificity
Detects human ROBO2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant rat ROBO1, recombinant human (rh) ROBO3 and rhROBO4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ROBO2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF3147 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ROBO2 Antibody [Unconjugated]

  • Drosophila) homolog 2
  • ROBO2
  • roundabout, axon guidance receptor, homolog 2 (Drosophila)
  • SAX3

Background

Human ROBO2 is a 175 kDa member of the ROBO/roundabout receptor family and is a 1357 amino acid (aa) type I transmembrane (TM) protein. It contains an extracellular domain (ECD) comprised of five C2-type Ig-like domains and three fibronectin type III domains. ROBO receptors interact with SLIT proteins to regulate axon guidance. Similar to ROBO1, the first two Ig-like domains of ROBO2 are predicted to be important for SLIT binding. Alternate splice forms of ROBO2 exist. The ROBO2 ECD in human shares 98% aa identity with the ROBO2 ECD in mouse and canine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Slit3 Antibody
(782619)
MAB3629
Immunohistochemistry Slit3 Antibody (782619)
(1 Publication)
Species: Mu
Applications: IHC
Slit2
[Unconjugated]
5444-SL
Slit2 [Unconjugated]
(13 Publications)
Species: Mu
Applications: BA
Slit1
[Unconjugated]
6514-SL
Slit1 [Unconjugated]
(2 Publications)
Species: Hu
Applications: BA
ROBO1 Antibody
AF1749
Immunohistochemistry ROBO1 Antibody
Immunohistochemistry ROBO1 Antibody
(11 Publications)
Species: Rt
Applications: IHC, WB
ROBO3 Antibody
AF3076
ROBO3 Antibody
(1 Review)
(23 Publications)
Species: Hu
Applications: IHC, WB
SHC2 Antibody
NBP1-87076
Immunohistochemistry-Paraffin SHC2 Antibody
Immunohistochemistry-Paraffin SHC2 Antibody
Immunohistochemistry-Paraffin SHC2 Antibody
Species: Hu
Applications: IHC, IHC-P, WB
Netrin-1
[Unconjugated]
1109-N1
SDS-PAGE Netrin-1 [Unconjugated]
(40 Publications)
Species: Mu
Applications: Bind
DCC Antibody
[Unconjugated]
AF844
Immunohistochemistry DCC Antibody [Unconjugated]
(1 Review)
(12 Publications)
Species: Mu
Applications: Block, IHC, WB
RIG-I Antibody
AF4859
Western Blot RIG-I Antibody
(2 Publications)
Species: Hu
Applications: WB
ROBO4 Antibody
NB110-58780
Immunohistochemistry ROBO4 Antibody
Western Blot ROBO4 Antibody
Immunocytochemistry/ Immunofluorescence ROBO4 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
VEGF [HRP]
DVE00
N/A VEGF [HRP]
N/A VEGF [HRP]
N/A VEGF [HRP]
(681 Publications)
Species: Hu
Applications: ELISA
Netrin-G1a
Antibody [Unconjugate
AF1166
Immunohistochemistry Netrin-G1a Antibody [Unconjugated]
Immunohistochemistry Netrin-G1a Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence Netrin-G1a Antibody [Unconjugated]
(7 Publications)
Species: Mu
Applications: WB
GDNF [Unconjugated]
212-GD
Bioactivity GDNF [Unconjugated]
SDS-PAGE GDNF [Unconjugated]
SEC-MALS GDNF [Unconjugated]
(217 Publications)
Species: Hu
Applications: Bind, BA
Leptin/OB [HRP]
DLP00
N/A Leptin/OB [HRP]
N/A Leptin/OB [HRP]
(155 Publications)
Species: Hu
Applications: ELISA
Cadherin-11
Antibody (283416)
MAB1790
Immunohistochemistry Cadherin-11 Antibody (283416)
Simple Western Cadherin-11 Antibody (283416)
Western Blot Cadherin-11 Antibody (283416)
(1 Review)
(11 Publications)
Species: Hu
Applications: IHC, Simple Western, WB
Prokineticin 2
Antibody
NBP2-43853
Immunocytochemistry/ Immunofluorescence Prokineticin 2 Antibody
(1 Publication)
Species: Bv, Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB
Neuropilin-1
Antibody [Unconjugate
AF566
Immunohistochemistry Neuropilin-1 Antibody [Unconjugated]
Immunohistochemistry Neuropilin-1 Antibody [Unconjugated]
Western Blot Neuropilin-1 Antibody [Unconjugated]
(10 Reviews)
(108 Publications)
Species: Mu, Rt
Applications: Block, CyTOF-ready, Flow, IHC, WB
SRGAP2 Antibody
NBP1-88830
Immunocytochemistry/ Immunofluorescence SRGAP2 Antibody
Immunohistochemistry-Paraffin SRGAP2 Antibody
Immunohistochemistry-Paraffin SRGAP2 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Semaphorin 3A
[Unconjugated]
1250-S3
Bioactivity Semaphorin 3A [Unconjugated]
(34 Publications)
Species: Hu
Applications: BA, BA
ROBO2 Antibody
[Unconjugated]
AF3147
ROBO2 Antibody [Unconjugated]
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC/IF

Publications for ROBO2 Antibody (AF3147)(10)

We have publications tested in 3 confirmed species: Human, Mouse, Chicken.

We have publications tested in 4 applications: Flow Cytometry, IHC, IHC-Fr, Immunoprecipitation.


Filter By Application
Flow Cytometry
(1)
IHC
(3)
IHC-Fr
(1)
Immunoprecipitation
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(4)
Chicken
(1)
All Species
Showing Publications 1 - 10 of 10.
Publications using AF3147 Applications Species
Maud Wurmser, Mridula Muppavarapu, Christine Mary Tait, Christophe Laumonnerie, Luz María González-Castrillón, Sara Ivy Wilson Robo2 Receptor Gates the Anatomical Divergence of Neurons Derived From a Common Precursor Origin Frontiers in Cell and Developmental Biology 6/23/2021 [PMID: 34249921]
Pooja Hor, Justin K. Ichida, Zea Borok, Amy L. Ryan Protocol for Differentiation of Human iPSCs into Pulmonary Neuroendocrine Cells STAR Protocols 10/28/2020 [PMID: 33111106]
Max Koppers, Roberta Cagnetta, Toshiaki Shigeoka, Lucia CS Wunderlich, Pedro Vallejo-Ramirez, Julie Qiaojin Lin et al. Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons eLife 11/20/2019 [PMID: 31746735]
M Gorla, C Santiago, K Chaudhari, AAK Layman, PM Oliver, GJ Bashaw Ndfip Proteins Target Robo Receptors for Degradation and Allow Commissural Axons to Cross the Midline in the Developing Spinal Cord Cell Rep, 2019-03-19;26(12):3298-3312.e4. 2019-03-19 [PMID: 30893602] (Immunoprecipitation, Mouse) Immunoprecipitation Mouse
Adrián Cárdenas, Ana Villalba, Camino de Juan Romero, Esther Picó, Christina Kyrousi, Athanasia C. Tzika et al. Evolution of Cortical Neurogenesis in Amniotes Controlled by Robo Signaling Levels Cell 7/26/2018 [PMID: 29961574]
Geutskens SB, Hordijk PL, van Hennik PB The chemorepellent Slit3 promotes monocyte migration. J. Immunol., 2010-11-15;185(12):7691-8. 2010-11-15 [PMID: 21078908] (Flow Cytometry, Human) Flow Cytometry Human
Takeuchi H, Inokuchi K, Aoki M Sequential arrival and graded secretion of Sema3F by olfactory neuron axons specify map topography at the bulb. Cell, 2010-06-11;141(6):1056-67. 2010-06-11 [PMID: 20550939] (IHC, Mouse) IHC Mouse
Reeber SL, Sakai N, Nakada Y, Dumas J, Dobrenis K, Johnson JE, Kaprielian Z Manipulating Robo expression in vivo perturbs commissural axon pathfinding in the chick spinal cord. J. Neurosci., 2008-08-27;28(35):8698-708. 2008-08-27 [PMID: 18753371] (IHC-Fr, Chicken) IHC-Fr Chicken
Nguyen-Ba-Charvet KT, Di Meglio T, Fouquet C, Chedotal A Robos and slits control the pathfinding and targeting of mouse olfactory sensory axons. J. Neurosci., 2008-04-16;28(16):4244-9. 2008-04-16 [PMID: 18417704] (IHC, Mouse) IHC Mouse
Andrews W, Barber M, Hernadez-Miranda LR, Xian J, Rakic S, Sundaresan V, Rabbitts TH, Pannell R, Rabbitts P, Thompson H, Erskine L, Murakami F, Parnavelas JG The role of Slit-Robo signaling in the generation, migration and morphological differentiation of cortical interneurons. Dev. Biol., 2007-11-13;313(2):648-58. 2007-11-13 [PMID: 18054781] (IHC, Mouse) IHC Mouse

Reviews for ROBO2 Antibody (AF3147) (0)

There are no reviews for ROBO2 Antibody (AF3147). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ROBO2 Antibody (AF3147) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional ROBO2 Products

Blogs on ROBO2

There are no specific blogs for ROBO2, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ROBO2 Antibody [Unconjugated] and receive a gift card or discount.


Bioinformatics

Gene Symbol ROBO2
Uniprot