| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3147-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3147-SP | |||
| Carrier Free | FAB3147V-100UG | |||
| Carrier Free | FAB3147U-100UG | |||
| Carrier Free | FAB3147T-100UG | |||
| Carrier Free | FAB3147S-100UG | |||
| Carrier Free | FAB3147R-100UG | |||
| Carrier Free | FAB3147N-100UG | |||
| Carrier Free | FAB3147G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3147 | |||
| Carrier Free | AF3147-SP | |||
![Immunohistochemistry: ROBO2 Antibody [NBP1-81399] - Anti-ROBO1 and anti-ROBO2 (NBP1-81399) staining in the corresponding KO embryos, showing the specificity of the antibodies. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31392959/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ROBO2-Antibody-Immunohistochemistry-NBP1-81399-img0017.jpg "Immunohistochemistry: ROBO2 Antibody [NBP1-81399] - Anti-ROBO1 and anti-ROBO2 (NBP1-81399) staining in the corresponding KO embryos, showing the specificity of the antibodies. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31392959/) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: ROBO2 Antibody [NBP1-81399] - Staining of human cerebral cortex shows moderate positivity in neuronal processes in neuropil.](http://images.novusbio.com/fullsize/ROBO2-Antibody-Immunohistochemistry-Paraffin-NBP1-81399-img0012.jpg)
![Immunohistochemistry-Paraffin: ROBO2 Antibody [NBP1-81399] - Staining of human kidney shows negative to very weak cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/ROBO2-Antibody-Immunohistochemistry-Paraffin-NBP1-81399-img0013.jpg)
![Immunohistochemistry-Paraffin: ROBO2 Antibody [NBP1-81399] - Staining of human liver shows very weak cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/ROBO2-Antibody-Immunohistochemistry-Paraffin-NBP1-81399-img0014.jpg)
![Immunohistochemistry-Paraffin: ROBO2 Antibody [NBP1-81399] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/ROBO2-Antibody-Immunohistochemistry-Paraffin-NBP1-81399-img0015.jpg)
![Immunohistochemistry-Paraffin: ROBO2 Antibody [NBP1-81399] - Staining of mouse embryo E14 shows strong positivity in developing olfactory bulb and olfactory nerve.](http://images.novusbio.com/fullsize/ROBO2-Antibody-Immunohistochemistry-Paraffin-NBP1-81399-img0016.jpg)
![Immunohistochemistry: ROBO2 Antibody [NBP1-81399] - Lateral positioning of ROBO1-expressing longitudinal axons in Nova1/2 dKO embryos.(A) Anti-ROBO1 staining of transverse sections of E12.5 lumbar spinal cords. The antibodies do not distinguish between isoforms. ROBO1 was expressed at a low level on precrossing axons & was highly upregulated on postcrossing axons in both WT & Nova1/2 dKO embryos. ROBO1-expressing axons displayed the same lateral positioning defect as anti-L1 labeled axons (compare with Figure 4B). Reducing Robo1(e6b+) partially rescued the lateral positioning defect in Nova1/2 dKO embryos, while reducing Robo2(e6b+) alone did not rescue. Reducing Robo1/2(e6b+) together further rescued the defect. Arrows indicate the lateral funiculus (LF) & ventral funiculus (VF). Scale bar, 50 μm. (B) Quantification of the lateral positioning of ROBO1-expressing axons in A. Data are represented as the mean ± SD (one-way ANOVA & Bonferroni post test; animal numbers & p values are indicated; ns, not significant). (C) Anti-ROBO2 staining of transverse sections of E12.5 lumbar spinal cords. The antibodies do not distinguish between isoforms. ROBO2 was primarily expressed by axons in the lateral funiculi, & the overall patterns were comparable between the WT & Nova1/2 dKO embryos. (D) Anti-ROBO1 & anti-ROBO2 staining in the corresponding KO embryos, showing the specificity of the antibodies. The Robo1 KO was generated by trapping the protein product in intracellular compartments (Friedel et al., 2005). ROBO1 expression was seen in neuronal cell bodies but not in axons in Robo1 KO spinal cords, as previously reported (Long et al., 2004). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31392959), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-81399_rabbit-polyclonal-robo2-antibody-310202416121910.jpg)
![Western Blot: ROBO2 Antibody [NBP3-13364] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ROBO2 antibody (NBP3-13364) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ROBO2-Antibody-Western-Blot-NBP3-13364-img0001.jpg "Western Blot: ROBO2 Antibody [NBP3-13364] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ROBO2 antibody (NBP3-13364) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: ROBO2 Antibody [NBP3-13364] - ROBO2 antibody detects ROBO2 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ROBO2 stained by ROBO2 antibody (NBP3-13364) diluted at 1:500. Red: Tau, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/ROBO2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13364-img0003.jpg)
![Immunohistochemistry-Paraffin: ROBO2 Antibody [NBP3-13364] - ROBO2 antibody detects ROBO2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded neuroepithelium of mouse E10.5 embryo. Green: ROBO2 stained by ROBO2 antibody (NBP3-13364) diluted at 1:500. Red: SOX2, a neural progenitor marker, stained by SOX2 antibody [GT1352] (NBP2-19306) diluted at 1:250. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/ROBO2-Antibody-Immunohistochemistry-Paraffin-NBP3-13364-img0002.jpg)
![Western Blot: ROBO2 Antibody [NBP3-23374] - Western blot analysis of C2C12 and SYF mouse cell lines. The blots were probed with anti-Robo2 (C-terminal region) in the absence (-) or presence (+) of ROBO2 (C-terminal region) blocking peptide.](http://images.novusbio.com/fullsize/nbp3-23374_rabbit-robo2-pab-1992023185698.jpg "Western Blot: ROBO2 Antibody [NBP3-23374] - Western blot analysis of C2C12 and SYF mouse cell lines. The blots were probed with anti-Robo2 (C-terminal region) in the absence (-) or presence (+) of ROBO2 (C-terminal region) blocking peptide.")
![Western Blot: Robo2 Antibody [NBP1-59546] - Titration: 0.2-1 ug/ml, Positive Control: Human brain.](http://images.novusbio.com/fullsize/Robo2-Antibody-Western-Blot-NBP1-59546-img0003.jpg "Western Blot: Robo2 Antibody [NBP1-59546] - Titration: 0.2-1 ug/ml, Positive Control: Human brain.")
![Western Blot: ROBO2 Antibody [H00006092-B01P] - Analysis of ROBO2 expression in transfected 293T cell line by ROBO2 polyclonal antibody. Lane 1: ROBO2 transfected lysate(151.58 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ROBO2-Antibody-Western-Blot-H00006092-B01P-img0002.jpg "Western Blot: ROBO2 Antibody [H00006092-B01P] - Analysis of ROBO2 expression in transfected 293T cell line by ROBO2 polyclonal antibody. Lane 1: ROBO2 transfected lysate(151.58 KDa). Lane 2: Non-transfected lysate.")