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Senataxin Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Senataxin Antibody [NBP2-13300] - Staining of human cell line RH-30 shows localization to nucleus & cytokinetic bridge. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Senataxin Antibody [NBP2-13300] - Staining of human pancreas shows no positivity in exocrine glanular cells as expected.
Immunohistochemistry-Paraffin: Senataxin Antibody [NBP2-13300] - Staining of human testis shows strong nuclear positivity in a subset of cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Senataxin Antibody [NBP2-13300] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.
Immunohistochemistry-Paraffin: Senataxin Antibody [NBP2-13300] - Staining of human cerebellum shows weak cytoplasmic positivity in Purkinje cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Senataxin Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: CCWCTPGGASTIDFLKRYASNTPSGEFQTADEDLCYCLECVAEYHKARDELPFLHEVLWELETLRLINHFEKSMKAEIGDDDELYIVDNNGEMPLFDITGQDFEN
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SETX
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Senataxin Protein (NBP2-13300PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (86%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Senataxin Antibody

  • ALS4recessive, non-Friedreich type 1
  • AOA2FLJ43459
  • DKFZp781B151
  • KIAA0625FLJ12840
  • senataxin
  • SETX

Background

Mutations in Senataxin result in the neurodegenerative disorders Ataxia-Oculomotor Apraxia 2 (AOA2) and amyotrophic lateral sclerosis (ALS4). Senataxin bears an RNA/DNA helicase domain suggesting a role in DNA and RNA processing and has recently been found to be involved in the DNA damage response. Alternate names for Senataxin include SEN1 homolog, SETX, ALS4, KIAA0625, SCAR1, and AOA2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SETX