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TDP-43/TARDBP Antibody (2E2-D3)

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Western Blot: TDP-43/TARDBP Antibody (2E2-D3) [H00023435-M01] - TARDBP monoclonal antibody (M01), clone 2E2-D3 Analysis of TARDBP expression in A-431.
Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody (2E2-D3) [H00023435-M01] - TARDBP in rat tissue samples. Image courtsey of product review by Daniel Pirici.
Western Blot: TDP-43/TARDBP Antibody (2E2-D3) [H00023435-M01] - PDI co-precipitates with TDP-43/TARDBP and is increased in mutant TDP-43 transgenic mouse spinal cords. Co-immunoprecipitation of wildtype and mutant Q331K ...read more
Immunocytochemistry/ Immunofluorescence: TDP-43/TARDBP Antibody (2E2-D3) [H00023435-M01] - Analysis of monoclonal antibody to TARDBP on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody (2E2-D3) [H00023435-M01] - TARDBP on formalin-fixed paraffin-embedded human leiomyosarcoma. [antibody concentration 3 ug/ml]
Western Blot: TDP-43/TARDBP Antibody (2E2-D3) [H00023435-M01] - Analysis of TARDBP expression in transfected 293T cell line by TARDBP monoclonal antibody (M01), clone 2E2-D3.Lane 1: TARDBP transfected lysate(44.7 ...read more
Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody (2E2-D3) [H00023435-M01] - Analysis of monoclonal antibody to TARDBP on formalin-fixed paraffin-embedded human leiomyosarcoma tissue Antibody concentration 3 ug/ml.
Genetic Strategies: Knockdown Validated: TDP-43/TARDBP Antibody (2E2-D3) [H00023435-M01] - Analysis of TARDBP over-expressed 293 cell line, cotransfected with TARDBP Validated Chimera RNAi ( Cat # H00023435-R01V ...read more
ELISA: TDP-43/TARDBP Antibody (2E2-D3) [H00023435-M01] - Detection limit for recombinant GST tagged TARDBP is 0.3 ng/ml as a capture antibody.
SINE compounds partially rescue motor deficits in an animal model of ALS/FTD. (b) Western blot demonstrating the expression of human TDP43 within the brains of AAV9-injected animals, which we have previously shown is ...read more
CLIP shows that TDP-43 directly interacts with translational target mRNAs. (C and D) Plots showing percentage of input mRNA in the TDP-43 IP for the mRNAs indicated in three separate CLIP assays from MN1 cells. ...read more
PDI co-precipitates with TDP-43 and is increased in mutant TDP-43 transgenic mouse spinal cords.(A) Co-immunoprecipitation of wildtype and mutant Q331K TDP-43-mCherry from Neuro2a cell lysates using an anti-PDI antibody ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ba, I, Pm, PpSpecies Glossary
Applications WB, ELISA, Func, ICC/IF, IHC, IP, KD
Clone
2E2-D3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

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TDP-43/TARDBP Antibody (2E2-D3) Summary

Immunogen
TARDBP (NP_031401.1, 1 a.a. ~ 260 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSEYIRVTEDENDEPIEIPSEDDGTVLLSTVTAQFPGACGLRYRNPVSQCMRGVRLVEGILHAPDAGWGNLVYVVNYPKDNKRKMDETDASSAVKVKRAVQKTSDLIVLGLPWKTTEQDLKEYFSTFGEVLMVQVKKDLKTGHSKGFGFVRFTEYETQVKVMSQRHMIDGRWCDCKLPNSKQSQDEPLRSRKVFVGRCTEDMTEDELREFFSQYGDVMDVFIPKPFRAFAFVTFADDQIAQSLCGEDLIIKGISVHISNA
Localization
H00023435-M01 Nucleus.
Specificity
TARDBP - TAR DNA binding protein
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TARDBP
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Functional
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Knockdown Validated
  • Western Blot 1:500
Application Notes
Cross reactivity in Mouse and Rat reported by customer review.
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 3 Reviews rated 5
using
H00023435-M01 in the following applications:

Publications
Read Publications using
H00023435-M01 in the following applications:

  • 4 publications
  • 3 publications
  • IHC
    2 publications
  • 5 publications
  • IP
    2 publications
  • WB
    9 publications

Reactivity Notes

Cross reactivity in Mouse and Rat reported by customer review. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TDP-43/TARDBP Antibody (2E2-D3)

  • ALS10
  • ALS10TAR DNA-binding protein-43
  • TAR DNA binding protein
  • TARDBP
  • TDP43
  • TDP-43
  • TDP-43TAR DNA-binding protein 43

Background

HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ELISA, Func, ICC/IF, IHC, IP, KD

Publications for TDP-43/TARDBP Antibody (H00023435-M01)(211)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: ICC/IF, IF/IHC, IHC, IHC-P, IP, WB.


Filter By Application
ICC/IF
(4)
IF/IHC
(3)
IHC
(2)
IHC-P
(5)
IP
(2)
WB
(9)
All Applications
Filter By Species
Human
(10)
Mouse
(7)
Rat
(1)
All Species
Showing Publications 1 - 10 of 211. Show All 211 Publications.
Publications using H00023435-M01 Applications Species
Mamontova, EM;Clément, MJ;Sukhanova, MV;Joshi, V;Bouhss, A;Rengifo-Gonzalez, JC;Desforges, B;Hamon, L;Lavrik, OI;Pastré, D; FUS RRM regulates poly(ADP-ribose) levels after transcriptional arrest and PARP-1 activation on DNA damage Cell reports 2023-10-05 [PMID: 37804508]
Saheed A, Bin B, Emily T et al. Hyperglycemia and O-GlcNAc transferase activity drive a cancer stem cell pathway in triple-negative breast cancer. Cancer Cell Int. 2023-05-25 [PMID: 37231419]
Carmen-Orozco RP, Tsao W, Ye Y et al. Elevated nuclear TDP-43 induces constitutive exon skipping bioRxiv : the preprint server for biology 2023-05-12 [PMID: 37215013] (IHC, Mouse) IHC Mouse
Mandy K, Lale G, Renee S et al. Neuronal overexpression of hTDP-43 in Caenorhabditis elegans mimics the cellular pathology commonly observed in TDP-43 proteinopathies. MicroPubl Biol. 2023-04-19 [PMID: 37159575]
Toshihide T, Kazuhiro M, Xin D et al. Sustained therapeutic benefits by transient reduction of TDP-43 using ENA-modified antisense oligonucleotides in ALS/FTD mice. Mol Ther Nucleic Acids. 2023-01-16 [PMID: 36817728]
Ellen G, Simone B, Carlos N et al. Sporadic Creutzfeldt-Jakob disease VM1: phenotypic and molecular characterization of a novel subtype of human prion disease. Acta Neuropathol Commun. 2022-08-17 [PMID: 35978418]
Johnathan C, Thomas J, Emily F et al. Atypical TDP-43 protein expression in an ALS pedigree carrying a p.Y374X truncation mutation in TARDBP. Brain Pathol. 2022-07-24 [PMID: 35871544]
T Liu, JA Woo, MZ Bukhari, X Wang, Y Yan, SC Buosi, A Ermekbaeva, A Sista, P Kotsiviras, P LePochat, A Chacko, X Zhao, DE Kang Modulation of synaptic plasticity, motor unit physiology, and TDP-43 pathology by CHCHD10 Acta neuropathologica communications, 2022-07-04;10(1):95. 2022-07-04 [PMID: 35787294]
Krach F, Wheeler EC, Regensburger M Et al. Aberrant NOVA1 function disrupts alternative splicing in early stages of amyotrophic lateral sclerosis Acta Neuropathol 2022-07-01 [PMID: 35778567] (IHC-P, Human)

Details:
Citation using the Alexa Fluor 405 version of this antibody.
IHC-P Human
Jorge M, Friederike H, Felix Z et al. Sirtuin-1 sensitive lysine-136 acetylation drives phase separation and pathological aggregation of TDP-43. Nat Commun. 2022-03-09 [PMID: 35264561]
Show All 211 Publications.

Reviews for TDP-43/TARDBP Antibody (H00023435-M01) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.

Reviews using H00023435-M01:
Filter by Applications
WB
(2)
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
WB Human 01/20/2022
View

Summary

ApplicationWestern Blot
Sample Tested293T cell line
SpeciesHuman
Lot2E2-D3
Immunohistochemistry-Paraffin TDP-43/TARDBP H00023435-M01
Enlarge
5
reviewed by:
Verified Customer
IHC-P 10/02/2012
View

Summary

ApplicationImmunohistochemistry-Paraffin
Western Blot TDP-43/TARDBP H00023435-M01
Enlarge
5
reviewed by:
Helen Chiang
WB Mouse 06/28/2012
View

Summary

ApplicationWestern Blot
Sample TestedMouse Brain Tissue
SpeciesMouse
Lot12076-S1

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TDP-43/TARDBP Antibody (H00023435-M01) (0)

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Recent Reviews

5
5
3
4
0
3
0
2
0
1
0

Verified Customer
01/20/2022
Application: WB
Species: Human

Verified Customer
10/02/2012
Application: IHC-P
Species:

Helen Chiang
06/28/2012
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol TARDBP
Entrez
OMIM
Uniprot