![Western Blot: Senataxin Antibody [NB100-57543] - Detection of Human Senataxin by Western Blot. Samples: Whole cell lysate (15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Senataxin antibody NB100-57543 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Senataxin-Antibody-Western-Blot-NB100-57543-img0002.jpg "Western Blot: Senataxin Antibody [NB100-57543] - Detection of Human Senataxin by Western Blot. Samples: Whole cell lysate (15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Senataxin antibody NB100-57543 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IP, ChIP, KD, KO |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 2627 and 2677 of human senataxin (NP_055861.2). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SETX |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Use in chromatin immunoprecipitation reported in scientific literature (PMID 26700805). |
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| Theoretical MW | 302 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
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| Publications using NB100-57543 | Applications | Species |
|---|---|---|
| Zhao DY, Gish G, Braunschweig U et al. SMN and symmetric arginine dimethylation of RNA polymerase II C-terminal domain control termination. Nature. 2016-01-07 [PMID: 26700805] (IP, Chemotaxis, Human) | IP, Chemotaxis | Human |
| Zhao D, Ni Z, Hu S et al. Regulation of transcription termination by FUS and TDP-43 bioRxiv 4373-01-01 (IP, Chemotaxis, Human) | IP, Chemotaxis | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for Senataxin Antibody (NB100-57543)Find related products by research area.
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![Immunoprecipitation: Senataxin Antibody [NB100-57543] - Detection of human Senataxin by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Senataxin antibody NB100-57543 used for IP at 6 ug per reaction. Senataxin was also immunoprecipitated by rabbit anti-Senataxin antibody NB100-57542. For blotting immunoprecipitated Senataxin, NB100-57543 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Senataxin-Antibody-Immunoprecipitation-NB100-57543-img0003.jpg "Immunoprecipitation: Senataxin Antibody [NB100-57543] - Detection of human Senataxin by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Senataxin antibody NB100-57543 used for IP at 6 ug per reaction. Senataxin was also immunoprecipitated by rabbit anti-Senataxin antibody NB100-57542. For blotting immunoprecipitated Senataxin, NB100-57543 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
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