![Knockout Validated: S100A4 Antibody (1C4) [NBP2-36431] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and S100A4 knockout (KO) HeLa cell line. PVDF membrane was probed with Mouse Anti-Human S100A4 Monoclonal Antibody (Catalog # NBP2-36431) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for S100A4 at approximately 12 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/S100A4-Antibody-1C4-Knockout-Validated-NBP2-36431-img0010.jpg "Knockout Validated: S100A4 Antibody (1C4) [NBP2-36431] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and S100A4 knockout (KO) HeLa cell line. PVDF membrane was probed with Mouse Anti-Human S100A4 Monoclonal Antibody (Catalog # NBP2-36431) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for S100A4 at approximately 12 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, KO |
| Clone | 1C4 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | Partial recombinant human S100A4 protein (between amino acids 1-200) [UniProt P26447]. |
| Isotype | IgG2b Lambda |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | S100A4 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 11.7 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein G purified |
![Immunocytochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunocytochemistry_15885.jpg)
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Secondary Antibodies |
Isotype Controls |
Research Areas for S100A4 Antibody (NBP2-36431)Find related products by research area.
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Epithelial-Mesenchymal Transition (EMT) Markers Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a... Read full blog post. |
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![Western Blot: S100A4 Antibody (1C4) [NBP2-36431] - WB analysis of S100A4 recombinant protein with S100A4 antibody (clone 1C4) at 0.5 ug/ml concentration.](http://images.novusbio.com/fullsize/S100A4-Antibody-1C4-Western-Blot-NBP2-36431-img0004.jpg "Western Blot: S100A4 Antibody (1C4) [NBP2-36431] - WB analysis of S100A4 recombinant protein with S100A4 antibody (clone 1C4) at 0.5 ug/ml concentration.")
![Immunocytochemistry/Immunofluorescence: S100A4 Antibody (1C4) [NBP2-36431] - S100A4 (1C4) antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/S100A4-Antibody-1C4-Immunocytochemistry-Immunofluorescence-NBP2-36431-img0002.jpg "Immunocytochemistry/Immunofluorescence: S100A4 Antibody (1C4) [NBP2-36431] - S100A4 (1C4) antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).")
![Immunohistochemistry-Paraffin: S100A4 Antibody (1C4) [NBP2-36431] - Analysis of tissue section of normal human spleen with mouse monoclonal S100A4 antibody (clone 1C4) at 5 ug/ml concentration.](http://images.novusbio.com/fullsize/S100A4-Antibody-1C4-Immunohistochemistry-Paraffin-NBP2-36431-img0009.jpg "Immunohistochemistry-Paraffin: S100A4 Antibody (1C4) [NBP2-36431] - Analysis of tissue section of normal human spleen with mouse monoclonal S100A4 antibody (clone 1C4) at 5 ug/ml concentration.")
![Flow Cytometry: S100A4 Antibody (1C4) [NBP2-36431] - An intracellular stain was performed on THP-1 cells with S100A4 (1C4) Antibody NBP2-36431 and a matched isotype control. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550.](http://images.novusbio.com/fullsize/S100A4-Antibody-1C4-Flow-Cytometry-NBP2-36431-img0012.jpg "Flow Cytometry: S100A4 Antibody (1C4) [NBP2-36431] - An intracellular stain was performed on THP-1 cells with S100A4 (1C4) Antibody NBP2-36431 and a matched isotype control. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550.")
![Western Blot: S100A4 Antibody (1C4) [NBP2-36431] - WB analysis of S100A4 in lysates of A375 and A549 cell lines with S100A4 antibody (clone 1C4). The antibody detected the endogenous protein at its expected molecular weight position 11.7 kDa.](http://images.novusbio.com/fullsize/S100A4-Antibody-1C4-Western-Blot-NBP2-36431-img0003.jpg "Western Blot: S100A4 Antibody (1C4) [NBP2-36431] - WB analysis of S100A4 in lysates of A375 and A549 cell lines with S100A4 antibody (clone 1C4). The antibody detected the endogenous protein at its expected molecular weight position 11.7 kDa.")
![Immunohistochemistry-Paraffin: S100A4 Antibody (1C4) [NBP2-36431] - Analysis of tissue section of human normal lung with mouse monoclonal S100A4 antibody (clone 1C4) at 5 ug/ml concentration. Majority of the cells of respiratory alveoli showed the cytoplasmic staining whereas some clusters of cells (lymphocytes, macrophages) developed nuclear staining also.](http://images.novusbio.com/fullsize/S100A4-Antibody-1C4-Immunohistochemistry-Paraffin-NBP2-36431-img0005.jpg "Immunohistochemistry-Paraffin: S100A4 Antibody (1C4) [NBP2-36431] - Analysis of tissue section of human normal lung with mouse monoclonal S100A4 antibody (clone 1C4) at 5 ug/ml concentration. Majority of the cells of respiratory alveoli showed the cytoplasmic staining whereas some clusters of cells (lymphocytes, macrophages) developed nuclear staining also.")
![Immunohistochemistry-Paraffin: S100A4 Antibody (1C4) [NBP2-36431] - Aanalysis of tissue section of Squamous Cell Carcinoma of Human Lungs with mouse monoclonal S100A4 antibody (clone 1C4) at 5 ug/ml concentration. An expected cytoplasmic as well as nuclear staining was observed in the tissue and the staining was found to be more intense in several cells which are potentially the advanced cancer cells, lymphocytes or infiltrating macrophages.](http://images.novusbio.com/fullsize/S100A4-Antibody-1C4-Immunohistochemistry-Paraffin-NBP2-36431-img0006.jpg "Immunohistochemistry-Paraffin: S100A4 Antibody (1C4) [NBP2-36431] - Aanalysis of tissue section of Squamous Cell Carcinoma of Human Lungs with mouse monoclonal S100A4 antibody (clone 1C4) at 5 ug/ml concentration. An expected cytoplasmic as well as nuclear staining was observed in the tissue and the staining was found to be more intense in several cells which are potentially the advanced cancer cells, lymphocytes or infiltrating macrophages.")
![Immunohistochemistry-Paraffin: S100A4 Antibody (1C4) [NBP2-36431] - Analysis of tissue section of human colon with mouse monoclonal S100A4 antibody (clone 1C4) at 5 ug/ml concentration. The columnar epithelial cells showed weak to negligible immunopositivity whereas the cells present in lamina propria, the loose cellular connective tissue in mucosa, developed intense staining in various type of cells.](http://images.novusbio.com/fullsize/S100A4-Antibody-1C4-Immunohistochemistry-Paraffin-NBP2-36431-img0007.jpg "Immunohistochemistry-Paraffin: S100A4 Antibody (1C4) [NBP2-36431] - Analysis of tissue section of human colon with mouse monoclonal S100A4 antibody (clone 1C4) at 5 ug/ml concentration. The columnar epithelial cells showed weak to negligible immunopositivity whereas the cells present in lamina propria, the loose cellular connective tissue in mucosa, developed intense staining in various type of cells.")
![Immunohistochemistry-Paraffin: S100A4 Antibody (1C4) [NBP2-36431] - Analysis of tissue section of normal human skeletal muscle with mouse monoclonal S100A4 antibody (clone 1C4) at 5 ug/ml concentration. The different myocytes showed a varying degree of cytoplasmic immuno-positivity whereas the staining was found to be intense in some of the peripherally placed nuclei.](http://images.novusbio.com/fullsize/S100A4-Antibody-1C4-Immunohistochemistry-Paraffin-NBP2-36431-img0008.jpg "Immunohistochemistry-Paraffin: S100A4 Antibody (1C4) [NBP2-36431] - Analysis of tissue section of normal human skeletal muscle with mouse monoclonal S100A4 antibody (clone 1C4) at 5 ug/ml concentration. The different myocytes showed a varying degree of cytoplasmic immuno-positivity whereas the staining was found to be intense in some of the peripherally placed nuclei.")
![Flow Cytometry: S100A4 Antibody (1C4) [NBP2-36431] - An intracellular stain was performed on HeLa cells with S100A4 (1C4) Antibody NBP2-36431 and a matched isotype control. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550.](http://images.novusbio.com/fullsize/S100A4-Antibody-1C4-Flow-Cytometry-NBP2-36431-img0011.jpg "Flow Cytometry: S100A4 Antibody (1C4) [NBP2-36431] - An intracellular stain was performed on HeLa cells with S100A4 (1C4) Antibody NBP2-36431 and a matched isotype control. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550.")
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