![Western Blot: RUNX1/CBFA2 Antibody (HL2211) - Azide and BSA Free [NBP3-25644] - Jurkat whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RUNX1 antibody [HL2211] (NBP3-25644) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25644_rabbit-runx1-cbfa2-mab-hl2211-azide-and-bsa-free-2120249351364.jpg "Western Blot: RUNX1/CBFA2 Antibody (HL2211) - Azide and BSA Free [NBP3-25644] - Jurkat whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RUNX1 antibody [HL2211] (NBP3-25644) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | HL2211 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant fragmemt of human RUNX1/CBFA2 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | RUNX1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
![Immunocytochemistry CCR7 Antibody (150503) [Unconjugated]](https://images.novusbio.com/images2/CCR7_MAB197_Immunocytochemistry_6580.jpg)
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Secondary Antibodies |
Isotype Controls |
Research Areas for RUNX1/CBFA2 Antibody (NBP3-25644)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | RUNX1 |
![Immunocytochemistry/Immunofluorescence: RUNX1/CBFA2 Antibody (HL2211) - Azide and BSA Free [NBP3-25644] - RUNX1 antibody [HL2211] detects RUNX1 protein at nucleus by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RUNX1 stained by RUNX1 antibody [HL2211] (NBP3-25644) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25644_rabbit-runx1-cbfa2-mab-hl2211-azide-and-bsa-free-21202493916.jpg "Immunocytochemistry/Immunofluorescence: RUNX1/CBFA2 Antibody (HL2211) - Azide and BSA Free [NBP3-25644] - RUNX1 antibody [HL2211] detects RUNX1 protein at nucleus by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RUNX1 stained by RUNX1 antibody [HL2211] (NBP3-25644) diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Western Blot: RUNX1/CBFA2 Antibody (HL2211) - Azide and BSA Free [NBP3-25644] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RUNX1 antibody [HL2211] (NBP3-25644) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25644_rabbit-runx1-cbfa2-mab-hl2211-azide-and-bsa-free-2120249362742.jpg "Western Blot: RUNX1/CBFA2 Antibody (HL2211) - Azide and BSA Free [NBP3-25644] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RUNX1 antibody [HL2211] (NBP3-25644) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Immunohistochemistry-Paraffin: RUNX1/CBFA2 Antibody (HL2211) - Azide and BSA Free [NBP3-25644] - RUNX1 antibody [HL2211] detects RUNX1 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. RUNX1 stained by RUNX1 antibody [HL2211] (NBP3-25644) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25644_rabbit-runx1-cbfa2-mab-hl2211-azide-and-bsa-free-2120249351368.jpg "Immunohistochemistry-Paraffin: RUNX1/CBFA2 Antibody (HL2211) - Azide and BSA Free [NBP3-25644] - RUNX1 antibody [HL2211] detects RUNX1 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. RUNX1 stained by RUNX1 antibody [HL2211] (NBP3-25644) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: RUNX1/CBFA2 Antibody (HL2211) - Azide and BSA Free [NBP3-25644] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with RUNX1 antibody [HL2211] (NBP3-25644) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25644_rabbit-runx1-cbfa2-mab-hl2211-azide-and-bsa-free-2120249342729.jpg "Western Blot: RUNX1/CBFA2 Antibody (HL2211) - Azide and BSA Free [NBP3-25644] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with RUNX1 antibody [HL2211] (NBP3-25644) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")