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RPB9 Antibody

Images

 
Western Blot: RPB9 Antibody [NBP1-92344] - Analysis in human cell line HEK 293.
Immunocytochemistry/ Immunofluorescence: RPB9 Antibody [NBP1-92344] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: RPB9 Antibody [NBP1-92344] - Staining of human cervix, uterine shows moderate nucleolus positivity in squamous epithelial cells.
Western Blot: RPB9 Antibody [NBP1-92344] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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RPB9 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: PDGTYEPGFVGIRFCQECNNMLYPKEDKENRILLYACRNCDYQQEADNSCIYVNKITHEVDELTQIIADVSQDPTLPRTEDHPCQKCGHKEAVFFQSHSA
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POLR2I
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
RPB9 Protein (NBP1-92344PEP)
Publications
Read Publication using NBP1-92344.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RPB9 Antibody

  • DNA-directed RNA polymerase II 14.5 kDa polypeptide
  • DNA-directed RNA polymerase II subunit I
  • DNA-directed RNA polymerase II subunit RPB9
  • hRPB14.5
  • polymerase (RNA) II (DNA directed) polypeptide I (14.5kD)
  • polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa
  • RNA polymerase II 14.5 kDa subunit
  • RNA polymerase II subunit B9
  • RPB14.5
  • RPB9

Background

RPB9 encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. The product of this gene has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RPB9 Antibody (NBP1-92344)(1)

We have publications tested in 1 application: WB.


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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RPB9 Antibody (NBP1-92344) (0)

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Secondary Antibodies

 

Isotype Controls

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Array NBP1-92344

Research Areas for RPB9 Antibody (NBP1-92344)

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Blogs on RPB9

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Bioinformatics

Gene Symbol POLR2I