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RPB8 Antibody

Images

 
Independent Antibodies: Western Blot: RPB8 Antibody [NBP1-80816] - Analysis using Anti-POLR2H antibody NBP1-80816 (A) shows similar pattern to independent antibody NBP2-76548 (B).
Orthogonal Strategies: Immunohistochemistry-Paraffin: RPB8 Antibody [NBP1-80816] - Staining in human thyroid gland and pancreas tissues using anti-POLR2H antibody. Corresponding POLR2H RNA-seq data are presented ...read more
Western Blot: RPB8 Antibody [NBP1-80816] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: RPB8 Antibody [NBP1-80816] - Staining of human pancreas shows low expression as expected.
Immunohistochemistry-Paraffin: RPB8 Antibody [NBP1-80816] - Staining of human thyroid gland shows high expression.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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RPB8 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: AGILFEDIFDVKDIDPEGKKFDRVSRLHCESESFKMDLILDVNIQIYPVDLGDKFRLVIASTLYEDGTLDDGEY
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POLR2H
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:2500-1:5000
  • Western Blot 0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
RPB8 Protein (NBP1-80816PEP)
Publications
Read Publication using
NBP1-80816 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RPB8 Antibody

  • DNA-directed RNA polymerases I, II, and III subunit RPABC3
  • hsRPB8
  • II, and III 17.1 kDa polypeptide
  • II, and III subunit ABC3
  • polymerase (RNA) II (DNA directed) polypeptide H
  • RPB8 homolog

Background

RPB8 encodes one of the essential subunits of RNA polymerase II that is shared by the other two eukaryotic DNA-directed RNA polymerases, I and III.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RPB8 Antibody (NBP1-80816)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC-P.


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IHC-P
(1)
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Mouse
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol POLR2H
Entrez
Uniprot