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POLR2G Antibody

Images

 
Western Blot: POLR2G Antibody [NBP1-32716] - Sample (30 ug of whole cell lysate)A: A43112% SDS PAGE, antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: POLR2G Antibody [NBP1-32716] - Paraffin-embedded mouse brain. POLR2G antibody diluted at 1:500
Immunohistochemistry-Paraffin: POLR2G Antibody [NBP1-32716] - Paraffin-embedded CL1-5 xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, RM, Sh, XpSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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POLR2G Antibody Summary

Immunogen
Full length human POLR2G Recombinant protein.
Localization
Nucleus
Predicted Species
Rat (100%), Sheep (99%), Xenopus (98%), Bovine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POLR2G
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation Assay dependent
  • Western Blot 1:500-1:3000
Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (98%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for POLR2G Antibody

  • DNA directed RNA polymerase II 19 kda polypeptide
  • DNA-directed RNA polymerase II 19 kDa polypeptide
  • DNA-directed RNA polymerase II subunit G
  • DNA-directed RNA polymerase II subunit RPB7
  • hRPB19
  • hsRPB7
  • MGC138369
  • polymerase (RNA) II (DNA directed) polypeptide G
  • RNA polymerase II 19 kDa subunit
  • RNA polymerase II seventh subunit
  • RNA polymerase II subunit B7
  • RPB19
  • RPB7MGC138367

Background

This gene encodes the seventh largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, the association of this subunit with the polymerase under suboptimal growth conditions indicates it may play a role in regulating polymerase function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for POLR2G Antibody (NBP1-32716) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for POLR2G Antibody (NBP1-32716). (Showing 1 - 1 of 1 FAQ).

  1. I found 4 antibodies against human POLR2G in your website: NBP1-32716, NBP1-37777, H00005436-B01 and H00005436-A01. Could you recommend one to do immunoprecipitation?
    • It looks like we have not validated immunoprecipitation for any of our POLR2G products. I therefore cannot offer any suggestion as to which product would be best suited for this assay. I can offer you our Innovator's Reward Program to any of the products you choose. In exchange for a review of our product in an application or species that has not been tested, we will issue you a credit for the purchase price of the antibody. See the following link for details: Innovator's Reward program. To help with your selection, I can say that the antibodies H00005436-B01 and H00005436-A01 are products that we distribute for a Taiwanese company called Abnova. We therefore do not do any in-house testing or development of their products. Abnova also does not offer any support for applications not listed on their datasheet. We can still offer you the Innovator's Reward on their products, but if you had any questions about the immunoprecipitation protocol or results, we would not be able to address them.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol POLR2G