![Western Blot: RAP74 Antibody (OTI4B10) [NBP3-17971] - Analysis of extracts (35ug) from 9 different cell lines by using anti-RAP74 monoclonal antibody.](http://images.novusbio.com/fullsize/RAP74-Antibody-OTI4B10-Western-Blot-NBP3-17971-img0005.jpg "Western Blot: RAP74 Antibody (OTI4B10) [NBP3-17971] - Analysis of extracts (35ug) from 9 different cell lines by using anti-RAP74 monoclonal antibody.")
| Reactivity | Hu, Mu, Rt, Ca, Pm, PmSpecies Glossary |
| Applications | WB, Flow, IHC |
| Clone | OTI4B10 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.44 mg/ml |
| Immunogen | Full length human recombinant protein of human GTF2F1(NP_002087) produced in HEK293T cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | GTF2F1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.44 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for RAP74 Antibody (NBP3-17971)Find related products by research area.
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| Gene Symbol | GTF2F1 |
![Immunohistochemistry-Paraffin: RAP74 Antibody (OTI4B10) [NBP3-17971] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-RAP74 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RAP74-Antibody-OTI4B10-Immunohistochemistry-Paraffin-NBP3-17971-img0004.jpg "Immunohistochemistry-Paraffin: RAP74 Antibody (OTI4B10) [NBP3-17971] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-RAP74 mouse monoclonal antibody.")
![Flow Cytometry: RAP74 Antibody (OTI4B10) [NBP3-17971] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RAP74 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/RAP74-Antibody-OTI4B10-Flow-Cytometry-NBP3-17971-img0006.jpg "Flow Cytometry: RAP74 Antibody (OTI4B10) [NBP3-17971] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RAP74 antibody, and then analyzed by flow cytometry.")
![Western Blot: RAP74 Antibody (OTI4B10) [NBP3-17971] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAP74 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAP74.](http://images.novusbio.com/fullsize/RAP74-Antibody-OTI4B10-Western-Blot-NBP3-17971-img0001.jpg "Western Blot: RAP74 Antibody (OTI4B10) [NBP3-17971] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAP74 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAP74.")
![Immunohistochemistry: RAP74 Antibody (OTI4B10) [NBP3-17971] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-RAP74 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RAP74-Antibody-OTI4B10-Immunohistochemistry-NBP3-17971-img0002.jpg "Immunohistochemistry: RAP74 Antibody (OTI4B10) [NBP3-17971] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-RAP74 mouse monoclonal antibody.")
![Immunohistochemistry: RAP74 Antibody (OTI4B10) [NBP3-17971] - Staining of paraffin-embedded Human endometrium tissue using anti-RAP74 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RAP74-Antibody-OTI4B10-Immunohistochemistry-NBP3-17971-img0003.jpg "Immunohistochemistry: RAP74 Antibody (OTI4B10) [NBP3-17971] - Staining of paraffin-embedded Human endometrium tissue using anti-RAP74 mouse monoclonal antibody.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")