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TFIISH Antibody (4E11)

Images

 
Western Blot: TFIISH Antibody (4E11) [H00006920-M08] - Analysis of TCEA3 expression in HepG2 (Cat # L019V1).
Immunocytochemistry/ Immunofluorescence: TFIISH Antibody (4E11) [H00006920-M08] - Analysis of monoclonal antibody to TCEA3 on HepG2 cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: TFIISH Antibody (4E11) [H00006920-M08] - Analysis of monoclonal antibody to TCEA3 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml
ELISA: TFIISH Antibody (4E11) [H00006920-M08] - Detection limit for recombinant GST tagged TCEA3 is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
4E11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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TFIISH Antibody (4E11) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
TCEA3 (AAH41613, 1 a.a. ~ 348 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGQEEELLRIAKKLEKMVARKNTEGALDLLKKLHSCQMSIQLLQTTRIGVAVNGVRKHCSDKEVVSLAKVLIKNWKRLLDSPGPPKGEKGEEREKAKKKEKGLECSDWKPEAGLSPPRKKREDPKTRRDSVDSKSSASSSPKRPSVERSNSSKSKAESPKTPSSPLTPTFASSMCLLAPCYLTGDSVRDKCVEMLSAALKADDDYKDYGVNCDKMASEIEDHIYQELKSTDMKYRNRVRSRISNLKDPRNPGLRRNVLSGAISAGLIAKMTAEEMASDELRELRNAMTQEAIREHQMAKTGGTTTDLFQCSKCKKKNCTYNQVQTRSADEPMTTFVLCNECGNRWKFC
Specificity
TCEA3 (4E11)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TCEA3
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TFIISH Antibody (4E11)

  • rhabdomyosarcoma antigen MU-RMS-40.22
  • TFIIS
  • TFIIS.H
  • TFIISH
  • transcription elongation factor A (SII), 3
  • transcription elongation factor A protein 3
  • Transcription elongation factor S-II protein 3
  • Transcription elongation factor TFIIS.h

Background

Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol TCEA3
Entrez
OMIM
Uniprot