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POLR3K Antibody

Images

 
Western Blot: POLR3K Antibody [NBP1-80815] - Analysis in control (vector only transfected HEK293T lysate) and POLR3K over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T ...read more
Immunohistochemistry-Paraffin: POLR3K Antibody [NBP1-80815] - Staining of human testis shows strong nuclear positivity in cells of seminiferus ducts.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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POLR3K Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: PGCGNGLIVEEGQRCHRFACNTCPYVHNITRKVTNRKYPKLKEVDDVLGGAAAWENVDSTAESCPKCEHPRAYFMQLQTRSADEPMTTFYKCCNAQCGHRWRD
Predicted Species
Mouse (99%), Rat (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POLR3K
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
POLR3K Protein (NBP1-80815PEP)
Publications
Read Publication using
NBP1-80815 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for POLR3K Antibody

  • C11
  • DNA-directed RNA polymerase III subunit K
  • DNA-directed RNA polymerase III subunit RPC10
  • DNA-directed RNA polymerases III 12.5 kDa polypeptide
  • hRPC11
  • HsC11p
  • polymerase (RNA) III (DNA directed) polypeptide K (12.3 kDa)
  • polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa
  • RNA polymerase III 12.5 kDa subunit
  • RNA polymerase III subunit (hRPC11)
  • RNA polymerase III subunit C10
  • RNA polymerase III subunit C11
  • RNA polymerase III subunit CII
  • RPC10
  • RPC11C11-RNP3
  • RPC12.5

Background

POLR3K encodes a small essential subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The carboxy-terminal domain of this subunit shares a high degree of sequence similarity to the carboxy-terminal domain of an RNA polymerase II elongation factor. This similarity in sequence is supported by functional studies showing that this subunit is required for proper pausing and termination during transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for POLR3K Antibody (NBP1-80815)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC-P.


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IHC-P
(1)
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Filter By Species
Mouse
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol POLR3K
Entrez