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Galanin Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Galanin Antibody [NBP2-33582] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Galanin Antibody [NBP2-33582] - Staining of human rectum shows moderate cytoplasmic positivity in peripheral nerve / ganglion.
Immunohistochemistry-Paraffin: Galanin Antibody [NBP2-33582] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: Galanin Antibody [NBP2-33582] - Staining of human pituitary gland shows moderate to strong cytoplasmic positivity in endocrine glandular cells.
Immunohistochemistry-Paraffin: Galanin Antibody [NBP2-33582] - Staining of human placenta shows no positivity in trophoblastic cells as expected.

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Galanin Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: GPHAVGNHRSFSDKNGLTSKRELRPEDDMKPGSFDRSIPENNIMRTIIEFLSFLHLKEAGALDRLLDLPAAASSEDIERS
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GAL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Galanin Protein (NBP2-33582PEP)
Reviewed Applications
Read 1 Review rated 4
using
NBP2-33582 in the following applications:

Publications
Read Publications using
NBP2-33582 in the following applications:

Reactivity Notes

Reactivity in monkey reported in scientific literature (PMID: 2967776).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Galanin Antibody

  • GAL
  • GAL1
  • galanin prepropeptide
  • Galanin
  • GALN
  • GALNgalanin-related peptide
  • GLNNgalanin-message-associated peptide
  • GMAP
  • MGC40167

Background

Galanin occurs in nerve fibers in most parts of the gastrointestinal tract and in nerve cell bodies in both the submucous and the myenteric plexus. Galanin has multiple effects on gut smooth muscle. Absorption with 10-100mg galanin 1-29 per ml diluted antiserum abolishes the staining, while galanin 1-10, VIP, NPY and Substance P do not.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Galanin Antibody (NBP2-33582)(2)

We have publications tested in 2 confirmed species: Monkey, Reptile.

We have publications tested in 1 application: IHC-Fr.


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IHC-Fr
(1)
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Filter By Species
Monkey
(1)
Reptile
(1)
All Species

Review for Galanin Antibody (NBP2-33582) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Macaca mulatta.

Reviews using NBP2-33582:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Macaca mulatta
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Galanin NBP2-33582
Enlarge
4
reviewed by:
Verified Customer
IHC-P Macaca mulatta 02/13/2017
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedrhesus monkey gingival epithelium
SpeciesMacaca mulatta
LotR59739

Comments

CommentsHIER = Diva Decloaker (pressure cooker)
Antibody Dilution = 1:1000
Incubation = 4°C overnight
Chromogen = DAB

Reference code NSCS16

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Galanin Antibody (NBP2-33582) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Blogs on Galanin

There are no specific blogs for Galanin, but you can read our latest blog posts.
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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Verified Customer
02/13/2017
Application: IHC-P
Species: Macaca mulatta

Bioinformatics

Gene Symbol GAL
Uniprot