![Western Blot: ROCK2 Antibody [NBP2-20199] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membranes were blotted with ROCK2 antibody [N3C1], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ROCK2-Antibody-Western-Blot-NBP2-20199-img0008.jpg "Western Blot: ROCK2 Antibody [NBP2-20199] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membranes were blotted with ROCK2 antibody [N3C1], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, Bv, RMSpecies Glossary |
| Applications | WB, ICC/IF, IHC, KD |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human ROCK2. The exact sequence is proprietary. |
| Localization | Cytoplasm (By similarity); Cell membrane; Peripheral membrane protein (By similarity) |
| Predicted Species | Bovine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ROCK2 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 161 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris-Glycine (pH7), 10% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
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Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: ROCK2 Antibody [NBP2-20199] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/ROCK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20199-img0005.jpg "Immunocytochemistry/Immunofluorescence: ROCK2 Antibody [NBP2-20199] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: ROCK2 Antibody [NBP2-20199] - Human esophagous cancer, using ROCK2 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ROCK2-Antibody-Immunohistochemistry-Paraffin-NBP2-20199-img0004.jpg "Immunohistochemistry-Paraffin: ROCK2 Antibody [NBP2-20199] - Human esophagous cancer, using ROCK2 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: ROCK2 Antibody [NBP2-20199] - Sample (30 ug of whole cell lysate) A: H1299 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/ROCK2-Antibody-Western-Blot-NBP2-20199-img0001.jpg "Western Blot: ROCK2 Antibody [NBP2-20199] - Sample (30 ug of whole cell lysate) A: H1299 5% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: ROCK2 Antibody [NBP2-20199] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 5% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/ROCK2-Antibody-Western-Blot-NBP2-20199-img0002.jpg "Western Blot: ROCK2 Antibody [NBP2-20199] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 5% SDS PAGE gel, diluted at 1:500.")
![Western Blot: ROCK2 Antibody [NBP2-20199] - Sample (50 ug of whole cell lysate) A: Rat brain, 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/ROCK2-Antibody-Western-Blot-NBP2-20199-img0003.jpg "Western Blot: ROCK2 Antibody [NBP2-20199] - Sample (50 ug of whole cell lysate) A: Rat brain, 5% SDS PAGE gel, diluted at 1:1000.")
![Knockdown Validated: ROCK2 Antibody [NBP2-20199] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody [N3C1], Internal.](http://images.novusbio.com/fullsize/ROCK2-Antibody-Western-Blot-NBP2-20199-img0006.jpg "Knockdown Validated: ROCK2 Antibody [NBP2-20199] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody [N3C1], Internal.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")