ROCK2 Antibodies
We offer ROCK2 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Proximity Ligation Assay, Sandwich ELISA, Western Blot. Each ROCK2 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our ROCK2 Antibodies can be used in a variety of model species: Bovine, Canine, Chicken, Chinese Hamster (Negative), Human, Mouse, Rat. Use the list below to choose the ROCK2 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our ROCK2 polyclonal antibodies and browse our ROCK2 monoclonal antibody catalog.
Alternate Names for ROCK2 Antibodies
anti-ROCK2 antibody, anti-coiled-coil-containing protein kinase 2 antibody, anti-EC 2.7.11 antibody, anti-EC 2.7.11.1 antibody, anti-KIAA0619 antibody, anti-p164 ROCK-2 antibody, anti-Rho kinase 2 antibody, anti-rho-associated protein kinase 2 antibody, anti-Rho-associated, coiled-coil containing protein kinase 2 antibody
14 Results for "rock2" in Primary Antibodies
![Western Blot: ROCK2 Antibody [NBP2-20199] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membranes were blotted with ROCK2 antibody [N3C1], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ROCK2-Antibody-Western-Blot-NBP2-20199-img0008.jpg "Western Blot: ROCK2 Antibody [NBP2-20199] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membranes were blotted with ROCK2 antibody [N3C1], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: ROCK2 Antibody [NBP2-20199] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/ROCK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20199-img0005.jpg)
![Immunohistochemistry-Paraffin: ROCK2 Antibody [NBP2-20199] - Human esophagous cancer, using ROCK2 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ROCK2-Antibody-Immunohistochemistry-Paraffin-NBP2-20199-img0004.jpg)
![Western Blot: ROCK2 Antibody [NBP2-20199] - Sample (30 ug of whole cell lysate) A: H1299 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/ROCK2-Antibody-Western-Blot-NBP2-20199-img0001.jpg)
![Western Blot: ROCK2 Antibody [NBP2-20199] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 5% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/ROCK2-Antibody-Western-Blot-NBP2-20199-img0002.jpg)
![Western Blot: ROCK2 Antibody [NBP2-20199] - Sample (50 ug of whole cell lysate) A: Rat brain, 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/ROCK2-Antibody-Western-Blot-NBP2-20199-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ROCK2 Antibody [NBP2-20199] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody [N3C1], Internal.](http://images.novusbio.com/fullsize/ROCK2-Antibody-Western-Blot-NBP2-20199-img0006.jpg)
![Western Blot: ROCK2 Antibody (1E12) [H00009475-M02] - ROCK2 monoclonal antibody (M02), clone 1E12 Analysis of ROCK2 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/ROCK2-Antibody-1E12-Western-Blot-H00009475-M02-img0009.jpg "Western Blot: ROCK2 Antibody (1E12) [H00009475-M02] - ROCK2 monoclonal antibody (M02), clone 1E12 Analysis of ROCK2 expression in Hela S3 NE.")
![Immunocytochemistry/Immunofluorescence: ROCK2 Antibody (1E12) [H00009475-M02] - Analysis of monoclonal antibody to ROCK2 on HeLa cell. Antibody concentration 30 ug/ml.](http://images.novusbio.com/fullsize/ROCK2-Antibody-1E12-Immunocytochemistry-Immunofluorescence-H00009475-M02-img0005.jpg)
![Immunohistochemistry-Paraffin: ROCK2 Antibody (1E12) [H00009475-M02] - Analysis of monoclonal antibody to ROCK2 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 1.5 ug/ml.](http://images.novusbio.com/fullsize/ROCK2-Antibody-1E12-Immunohistochemistry-Paraffin-H00009475-M02-img0007.jpg)
![Sandwich ELISA: ROCK2 Antibody (1E12) [H00009475-M02] - Detection limit for recombinant GST tagged ROCK2 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ROCK2-Antibody-1E12-Sandwich-ELISA-H00009475-M02-img0008.jpg)
![Western Blot: ROCK2 Antibody [NBP1-86124] - Analysis in human cell line RH-30.](http://images.novusbio.com/fullsize/ROCK2-Antibody-Western-Blot-NBP1-86124-img0010.jpg "Western Blot: ROCK2 Antibody [NBP1-86124] - Analysis in human cell line RH-30.")
![Immunohistochemistry-Paraffin: ROCK2 Antibody [NBP1-86124] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/ROCK2-Antibody-Immunohistochemistry-Paraffin-NBP1-86124-img0014.jpg)
![Western Blot: ROCK2 Antibody [NBP1-86124] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/ROCK2-Antibody-Western-Blot-NBP1-86124-img0009.jpg)
![Immunohistochemistry-Paraffin: ROCK2 Antibody [NBP1-86124] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ROCK2-Antibody-Immunohistochemistry-Paraffin-NBP1-86124-img0001.jpg)
![Immunohistochemistry-Paraffin: ROCK2 Antibody [NBP1-86124] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/ROCK2-Antibody-Immunohistochemistry-Paraffin-NBP1-86124-img0012.jpg)
![Immunohistochemistry-Paraffin: ROCK2 Antibody [NBP1-86124] - Staining of human colorectal cancer shows strong cytoplasmic positivity in tumor cells.](http://images.novusbio.com/fullsize/ROCK2-Antibody-Immunohistochemistry-Paraffin-NBP1-86124-img0013.jpg)
![Western Blot: ROCK2 Antibody (GT11712) [NBP3-13535] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membranes were blotted with ROCK2 antibody [GT11712] (NBP3-13535) diluted at 1:1000 and competitor's antibody diluted at 1:100. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ROCK2-Antibody-GT11712-Western-Blot-NBP3-13535-img0007.jpg "<b>Independent Antibodies Validation. </b>Western Blot: ROCK2 Antibody (GT11712) [NBP3-13535] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membranes were blotted with ROCK2 antibody [GT11712] (NBP3-13535) diluted at 1:1000 and competitor's antibody diluted at 1:100. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ROCK2 Antibody (GT11712) [NBP3-13535] - ROCK2 antibody [GT11712] detects ROCK2 protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. ROCK2 antibody [GT11712] (NBP3-13535) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/ROCK2-Antibody-GT11712-Immunohistochemistry-Paraffin-NBP3-13535-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ROCK2 Antibody (GT11712) [NBP3-13535] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody [GT11712] (NBP3-13535) diluted at 1:500.](http://images.novusbio.com/fullsize/ROCK2-Antibody-GT11712-Western-Blot-NBP3-13535-img0006.jpg)
![Immunohistochemistry-Paraffin: ROCK2 Antibody (GT11712) [NBP3-13535] - ROCK2 antibody [GT11712] detects ROCK2 protein at cytoplasm in human esophagus by immunohistochemical analysis. Sample: Paraffin-embedded human esophagus. ROCK2 antibody [GT11712] (NBP3-13535) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/ROCK2-Antibody-GT11712-Immunohistochemistry-Paraffin-NBP3-13535-img0002.jpg)
![Immunohistochemistry-Paraffin: ROCK2 Antibody (GT11712) [NBP3-13535] - ROCK2 antibody [GT11712] detects ROCK2 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. ROCK2 antibody [GT11712] (NBP3-13535) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/ROCK2-Antibody-GT11712-Immunohistochemistry-Paraffin-NBP3-13535-img0003.jpg)
![Immunohistochemistry-Paraffin: ROCK2 Antibody (GT11712) [NBP3-13535] - ROCK2 antibody [GT11712] detects ROCK2 protein at cytoplasm in mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. ROCK2 antibody [GT11712] (NBP3-13535) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/ROCK2-Antibody-GT11712-Immunohistochemistry-Paraffin-NBP3-13535-img0004.jpg)
![Western Blot: ROCK2 Antibody (GT11712) [NBP3-13535] - Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody [GT11712] (NBP3-13535) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13535_mouse-rock2-mab-gt11712-910202420115030.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: ROCK2 Antibody (GT11712) [NBP3-13535] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody [GT11712] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-13535_mouse-rock2-mab-gt11712-1610202419414559.jpg)
![Western Blot: ROCK2 Antibody [NBP1-76570] - Mouse brain tissue lysate with ROCK2 antibody at (A) 1 and (B) 2 ug/mL](http://images.novusbio.com/fullsize/ROCK2-Antibody-Western-Blot-NBP1-76570-img0001.jpg "Western Blot: ROCK2 Antibody [NBP1-76570] - Mouse brain tissue lysate with ROCK2 antibody at (A) 1 and (B) 2 ug/mL")
![Immunocytochemistry/ Immunofluorescence: ROCK2 Antibody - BSA Free [NBP1-76570] - Immunofluorescence of ROCK2 in mouse brain tissue with ROCK2 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76570_rabbit-polyclonal-rock2-antibody-271120242074683.jpg)
![Immunohistochemistry: ROCK2 Antibody - BSA Free [NBP1-76570] - Immunohistochemistry of ROCK2 in mouse brain tissue with ROCK2 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76570_rabbit-polyclonal-rock2-antibody-2711202420324878.jpg)
![Western Blot: ROCK2 Antibody (JE31-36) [NBP3-32914] - Western blot analysis of ROCK2 on different lysates with Rabbit anti-ROCK2 antibody (NBP3-32914) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: MCF7 cell lysate Lane 3: C2C12 cell lysate Lane 4: C6 cell lysate Lane 5: Mouse brain tissue lysate Lane 6: Rat brain tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 161 kDa Observed band size: 150 kDa Exposure time: 59 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32914) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32914_rabbit-rock2-mab-je31-36-288202412461668.jpg "Western Blot: ROCK2 Antibody (JE31-36) [NBP3-32914] - Western blot analysis of ROCK2 on different lysates with Rabbit anti-ROCK2 antibody (NBP3-32914) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: MCF7 cell lysate Lane 3: C2C12 cell lysate Lane 4: C6 cell lysate Lane 5: Mouse brain tissue lysate Lane 6: Rat brain tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 161 kDa Observed band size: 150 kDa Exposure time: 59 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32914) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: ROCK2 Antibody (JE31-36) [NBP3-32914] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-ROCK2 antibody (NBP3-32914) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32914) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32914_rabbit-rock2-mab-je31-36-298202414262644.jpg)
![Immunocytochemistry/ Immunofluorescence: ROCK2 Antibody (JE31-36) [NBP3-32914] - Immunocytochemistry analysis of HeLa cells labeling ROCK2 with Rabbit anti-ROCK2 antibody (NBP3-32914) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ROCK2 antibody (NBP3-32914) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32914_rabbit-rock2-mab-je31-36-29202410334061.jpg)
![Western Blot: ROCK2 Antibody (8G3U2) [NBP3-16123] - Western blot analysis of extracts of various cell lines, using ROCK2 Rabbit mAb (NBP3-16123) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/ROCK2-Antibody-8G3U2-Western-Blot-NBP3-16123-img0002.jpg "Western Blot: ROCK2 Antibody (8G3U2) [NBP3-16123] - Western blot analysis of extracts of various cell lines, using ROCK2 Rabbit mAb (NBP3-16123) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunohistochemistry-Paraffin: ROCK2 Antibody (8G3U2) [NBP3-16123] - Analysis of paraffin-embedded Rat colon tissue using ROCK2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16123_rabbit-rock2-mab-8g3u2-immunohistochemistry-paraffin-71202584338..png)
![Immunohistochemistry-Paraffin: ROCK2 Antibody (8G3U2) [NBP3-16123] - Analysis of paraffin-embedded Mouse intestin tissue using ROCK2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16123_rabbit-rock2-mab-8g3u2-immunohistochemistry-paraffin-71202584440..png)
![Western Blot: ROCK2 Antibody [NB100-593] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ROCK2 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/ROCK2-Antibody-Western-Blot-NB100-593-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: ROCK2 Antibody [NB100-593] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ROCK2 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: ROCK2 Antibody [NB100-593] - Detection of Human Rho Kinase Alpha on HeLa whole cell lysate using NB100-593. NB100-593 and NB100-592 were used at 5 ug/mg lysate to immunoprecipitate Rho Kinase Alpha.](http://images.novusbio.com/fullsize/ROCK2-Antibody-Western-Blot-NB100-593-img0002.jpg)
![Western Blot: ROCK2 [p Ser1366] Antibody (HL1071) [NBP3-13711] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 (phospho Ser1366) antibody [HL1071] (NBP3-13711) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ROCK2-p-Ser1366-Antibody-HL1071-Western-Blot-NBP3-13711-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: ROCK2 [p Ser1366] Antibody (HL1071) [NBP3-13711] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 (phospho Ser1366) antibody [HL1071] (NBP3-13711) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ROCK2 [p Ser1366] Antibody (HL1071) [NBP3-13711] - ROCK2 (phospho Ser1366) antibody detects ROCK2 (phospho Ser1366) protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ROCK2 (phospho Ser1366) stained by ROCK2 (phospho Ser1366) antibody (NBP3-13711) diluted at 1:500.](http://images.novusbio.com/fullsize/ROCK2-p-Ser1366-Antibody-HL1071-Immunocytochemistry-Immunofluorescence-NBP3-13711-img0002.jpg)
![Western Blot: ROCK2 [p Ser1366] Antibody (HL1071) [NBP3-13711] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody [HL1071] (NBP3-13711) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13711_rabbit-rock2-p-ser-1366-mab-hl1071-91020242001032.jpg)
![Western Blot: ROCK2 Antibody (10E1) [NBP3-15152] - Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, L02 whole cell lysate, HepG2 whole cell lysate, Mouse Brain whole cell lysate, Rat Brain whole cell lysate. All lanes: ROCK2 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 161 KDa. Observed band size: 161 KDa.](http://images.novusbio.com/fullsize/ROCK2-Antibody-10E1-Western-Blot-NBP3-15152-img0002.jpg "Western Blot: ROCK2 Antibody (10E1) [NBP3-15152] - Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, L02 whole cell lysate, HepG2 whole cell lysate, Mouse Brain whole cell lysate, Rat Brain whole cell lysate. All lanes: ROCK2 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 161 KDa. Observed band size: 161 KDa.")
![Immunocytochemistry/Immunofluorescence: ROCK2 Antibody (10E1) [NBP3-15152] - Immunofluorescence staining of Hela Cells with at ROCK2 antibody 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/ROCK2-Antibody-10E1-Immunocytochemistry-Immunofluorescence-NBP3-15152-img0001.jpg)
![Western Blot: ROCK2 Antibody (S01-4F1) [NBP3-19508] - Western blot detection of ROCK2 in Hela, Rat Brain, K562 cell lysates using NBP3-19508 (1:1000 diluted). Predicted band size: 161kDa. Observed band size: 161kDa.](http://images.novusbio.com/fullsize/ROCK2-Antibody-S01-4F1-Western-Blot-NBP3-19508-img0002.jpg "Western Blot: ROCK2 Antibody (S01-4F1) [NBP3-19508] - Western blot detection of ROCK2 in Hela, Rat Brain, K562 cell lysates using NBP3-19508 (1:1000 diluted). Predicted band size: 161kDa. Observed band size: 161kDa.")
![Immunocytochemistry/Immunofluorescence: ROCK2 Antibody (S01-4F1) [NBP3-19508] - Immunofluorescence of ROCK2 (green) in Hela cells using NBP3-19508 at dilution 1/200, and DAPI (blue)](http://images.novusbio.com/fullsize/ROCK2-Antibody-S01-4F1-Immunocytochemistry-Immunofluorescence-NBP3-19508-img0001.jpg)