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ROCK2 Antibody (S01-4F1)

Images

 
Western Blot: ROCK2 Antibody (S01-4F1) [NBP3-19508] - Western blot detection of ROCK2 in Hela, Rat Brain, K562 cell lysates using NBP3-19508 (1:1000 diluted). Predicted band size: 161kDa. Observed band size: 161kDa.
Immunocytochemistry/ Immunofluorescence: ROCK2 Antibody (S01-4F1) [NBP3-19508] - Immunofluorescence of ROCK2 (green) in Hela cells using NBP3-19508 at dilution 1/200, and DAPI (blue)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clone
S01-4F1
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.3 mg/ml

Order Details

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ROCK2 Antibody (S01-4F1) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthetic peptide of human ROCK2 (Uniprot # O75116)
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
ROCK2
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1/50
  • Western Blot 1/2000
Theoretical MW
161 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
Preservative
0.01% Sodium Azide
Concentration
0.3 mg/ml
Purity
Affinity purified

Alternate Names for ROCK2 Antibody (S01-4F1)

  • coiled-coil-containing protein kinase 2
  • EC 2.7.11
  • EC 2.7.11.1
  • KIAA0619
  • p164 ROCK-2
  • Rho kinase 2
  • rho-associated protein kinase 2
  • Rho-associated, coiled-coil containing protein kinase 2
  • ROCK2
  • ROCK-II
  • ROKalpha

Background

ROCK2 is encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ROCK2 Antibody (NBP3-19508) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ROCK2 Antibody (NBP3-19508) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ROCK2