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RNA Polymerase II/POLR2A [p Thr4] Antibody [Alexa Fluor® 700]

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Product Details

Summary
Reactivity Hu, Ch, Dr, YeSpecies Glossary
Applications WB, DB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Alexa Fluor 700

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RNA Polymerase II/POLR2A [p Thr4] Antibody [Alexa Fluor® 700] Summary

Immunogen
A synthetic peptide made to a phosphorylated Threonine (position 4) of the CTD heptad in the human RNA polymerase II protein [UniProt P24928]
Modification
p Thr4
Localization
Nucleus.
Specificity
This antibody is specific for the phosphorylated Threonine found in position 4 of the RNA polymerase II CTD heptad.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POLR2A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Dot Blot
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for RNA Polymerase II/POLR2A [p Thr4] Antibody [Alexa Fluor® 700]

  • DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kdsubunit
  • DNA-directed RNA polymerase II subunit A
  • DNA-directed RNA polymerase II subunit RPB1
  • DNA-directed RNA polymerase III largest subunit
  • EC 2.7.7.48
  • EC 2.7.7.6
  • hRPB220
  • hsRPB1
  • MGC75453
  • POLR2
  • POLR2A
  • POLRA
  • polymerase (RNA) II (DNA directed) polypeptide A (220kD)
  • polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
  • RNA Pol 2
  • RNA Polymerase 2
  • RNA polymerase II subunit B1
  • RNA Polymerase II
  • RNA-directed RNA polymerase II subunit RPB1
  • RNAPII
  • RPB1
  • RPBh1
  • RpIILS
  • RPO2
  • RPOL2

Background

RNA polymerase II (RNAPII) is an essential component of the RNAP II transcription elongation complex. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the C-terminal repeat (YSPTSPS) domain of the largest subunit of RNAPII.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol POLR2A