Home » RNA Polymerase II/POLR2A » RNA Polymerase II/POLR2A Antibodies » RNA Polymerase II/POLR2A [p Ser5] Antibody (CTD 4H8) [mFluor Violet 500 SE]
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RNA Polymerase II/POLR2A [p Ser5] Antibody (CTD 4H8) [mFluor Violet 500 SE]

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RNA Polymerase II/POLR2A [p Ser5] Antibody (CTD 4H8) [mFluor Violet 500 SE] - Vial of mFluor Violet 500 conjugated antibody. mFluor Violet 500 is optimally excited at 410 nm by the Violet laser (405 nm) and has an emission maximum of 501 nm.
RNA Polymerase II/POLR2A [p Ser5] Antibody (CTD 4H8) [mFluor Violet 500 SE] - Vial of mFluor Violet 500 conjugated antibody. mFluor Violet 500 is optimally excited at 410 nm by the Violet laser (405 nm) and has an ...read more

Product Details

Summary
Reactivity Hu, YeSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IP, ChIP, CyTOF-ready
Clone
CTD 4H8
Clonality
Monoclonal
Host
Mouse
Conjugate
mFluor Violet 500 SE

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-50274AF350
Alexa Fluor 405
NBP2-50274AF405
Alexa Fluor 488
NBP2-50274AF488
Alexa Fluor 532
NBP2-50274AF532
Alexa Fluor 594
NBP2-50274AF594
Alexa Fluor 647
NBP2-50274AF647
Alexa Fluor 700
NBP2-50274AF700
Alexa Fluor 750
NBP2-50274AF750
Allophycocyanin
NBP2-50274APC
Biotin
NBP2-50274B
CoraFluor 1
NBP2-50274CL1
DyLight 350
NBP2-50274UV
DyLight 405
NBP2-50274V
DyLight 488
NBP2-50274G
DyLight 550
NBP2-50274R
DyLight 594
NBP2-50274DL594
DyLight 650
NBP2-50274C
DyLight 680
NBP2-50274FR
DyLight 755
NBP2-50274IR
FITC
NBP2-50274F
HRP
NBP2-50274H
Janelia Fluor 525
NBP2-50274JF525
Janelia Fluor 549
NBP2-50274JF549
Janelia Fluor 646
NBP2-50274JF646
Janelia Fluor 669
NBP2-50274JF669
PE
NBP2-50274PE
PerCP
NBP2-50274PCP
Unconjugated
NBP2-50274
mFluor Violet 450 SE
NBP2-50274MFV450
mFluor Violet 610 SE
NBP2-50274MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-50274
BSA Free
NBP2-50274
BSA Free
NBP2-50274-0.025ml
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RNA Polymerase II/POLR2A [p Ser5] Antibody (CTD 4H8) [mFluor Violet 500 SE] Summary

Immunogen
Peptide: 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser5
Modification
p Ser5
Specificity
Please note that this antibody detects both unphosphorylated and phosphorylated forms of the protein.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
POLR2A
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • Chromatin Immunoprecipitation Sequencing
  • CyTOF-ready
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Saccharomyces cerevisiae

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for RNA Polymerase II/POLR2A [p Ser5] Antibody (CTD 4H8) [mFluor Violet 500 SE]

  • DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kdsubunit
  • DNA-directed RNA polymerase II subunit A
  • DNA-directed RNA polymerase II subunit RPB1
  • DNA-directed RNA polymerase III largest subunit
  • EC 2.7.7.48
  • EC 2.7.7.6
  • hRPB220
  • hsRPB1
  • MGC75453
  • POLR2
  • POLR2A
  • POLRA
  • polymerase (RNA) II (DNA directed) polypeptide A (220kD)
  • polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
  • RNA Pol 2
  • RNA Polymerase 2
  • RNA polymerase II subunit B1
  • RNA Polymerase II
  • RNA-directed RNA polymerase II subunit RPB1
  • RNAPII
  • RPB1
  • RPBh1
  • RpIILS
  • RPO2
  • RPOL2

Background

RNA polymerase II (RNAPII) is an essential component of the RNAP II transcription elongation complex. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the C-terminal repeat (YSPTSPS) domain of the largest subunit of RNAPII.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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RNA Polymerase
II/POLR2A [p Ser5] An ...
NBP2-50274MFV500
RNA Polymerase II/POLR2A [p Ser5] Antibody (CTD 4H8) [mFluor Violet 500 SE]
Species: Hu, Ye
Applications: WB, ELISA, Flow, ICC/IF, IHC, IP, ChIP, CyTOF-ready

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Bioinformatics

Gene Symbol POLR2A