![Western Blot: RNA Polymerase II/POLR2A Antibody (CTD 4H8) [NBP2-50274] - Monoclonal antibody 4H8. RNA polymerase II (RNAP II) contains 12 subunits. The C-terminal domain of the largest subunit of RNA polymerase II (RNAP II) contains a highly conserved heptapeptide repeat, YSPTSPS. Monoclonal antibody 4H8 was obtained using synthetic peptide and specifically recognises the C-terminal domain of RNAP II.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-Antibody-CTD-4H8-Western-Blot-NBP2-50274-img0002.jpg "Western Blot: RNA Polymerase II/POLR2A Antibody (CTD 4H8) [NBP2-50274] - Monoclonal antibody 4H8. RNA polymerase II (RNAP II) contains 12 subunits. The C-terminal domain of the largest subunit of RNA polymerase II (RNAP II) contains a highly conserved heptapeptide repeat, YSPTSPS. Monoclonal antibody 4H8 was obtained using synthetic peptide and specifically recognises the C-terminal domain of RNAP II.")
| Reactivity | Hu, YeSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC, IP, ChIP, CyTOF-ready |
| Clone | CTD 4H8 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Peptide: 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser5 |
| Modification | p Ser5 |
| Specificity | Please note that this antibody detects both unphosphorylated and phosphorylated forms of the protein. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | POLR2A |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Positive control(s): HeLa cell line. This antibody is Cytof ready. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
![RNA Polymerase II/POLR2A [p Ser5] Antibody (CTD 4H8) - BSA Free](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "RNA Polymerase II/POLR2A [p Ser5] Antibody (CTD 4H8) - BSA Free")
Secondary Antibodies |
Isotype Controls |
Research Areas for RNA Polymerase II/POLR2A Antibody (NBP2-50274)Find related products by research area.
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| Gene Symbol | POLR2A |
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")