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RING1 Antibody (8C12F4) - BSA Free

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Western Blot: RING1 Antibody (8C12F4) [NBP2-37370] - Analysis using Ring1 mouse mAb against MOLT-4 (1), LNCaP (2), Hela (3), HEK-293 (4) and Jurkat (5) cell lysate.
Immunohistochemistry-Paraffin: RING1 Antibody (8C12F4) [NBP2-37370] - Analysis of rectum cancer tissues using Ring1 mouse mAb with DAB staining.
Flow Cytometry: RING1 Antibody (8C12F4) [NBP2-37370] - Analysis of Hela cells using Ring1 mouse mAb (green) and negative control (red).
Western Blot: RING1 Antibody (8C12F4) [NBP2-37370] - Analysis using Ring1 mAb against human Ring1 recombinant protein. (Expected MW is 44.6 kDa)
Western Blot: RING1 Antibody (8C12F4) [NBP2-37370] - Analysis using Ring1 mAb against HEK293 (1) and Ring1 (AA: 79-263)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry-Paraffin: RING1 Antibody (8C12F4) [NBP2-37370] - Analysis of cervical cancer tissues using Ring1 mouse mAb with DAB staining.
ELISA: RING1 Antibody (8C12F4) [NBP2-37370] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC, CyTOF-ready
Clone
8C12F4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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Catalog# & Formulation Size Price
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RING1 Antibody (8C12F4) - BSA Free Summary

Immunogen
Purified recombinant fragment of human RING1 (AA: 79-263) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RING1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
Application Notes
This antibody is Cytof ready.
Theoretical MW
42.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-37370 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for RING1 Antibody (8C12F4) - BSA Free

  • EC 6.3.2
  • EC 6.3.2.-
  • Polycomb complex protein RING1
  • Really interesting new gene 1 protein
  • ring finger protein 1RING1A
  • RING1
  • RNF1
  • RNF1E3 ubiquitin-protein ligase RING1

Background

RING1 belongs to the RING finger family, members of which encode proteins characterized by a RING domain, a zinc-binding motif related to the zinc finger domain. The gene product can bind DNA and can act as a transcriptional repressor. It is associated with the multimeric polycomb group protein complex. The gene product interacts with the polycomb group proteins BMI1, EDR1, and CBX4, and colocalizes with these proteins in large nuclear domains. It interacts with the CBX4 protein via its glycine-rich C-terminal domain. The gene maps to the HLA class II region, where it is contiguous with the RING finger genes FABGL and HKE4. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RING1 Antibody (NBP2-37370)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol RING1