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MEL-18 Antibody

Images

 
Western Blot: MEL-18 Antibody [NB100-1240] - A549 (A) and PD19 (B) cell lysate (RIPA buffer, 30ug total protein per lane). Detected by chemiluminescence.
Western Blot: MEL-18 Antibody [NB100-1240] - Analysis of MEL-18 in Human Lung lysate using this antibody at 1 ug/ ml.

Product Details

Summary
Reactivity Hu, Bv, CaSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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MEL-18 Antibody Summary

Immunogen
Peptide with sequence C-KMTVNGAPVPPLT corresponding to C-Terminus according to NP_009075.1.
Predicted Species
Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PCGF2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 2 ug/ml
Application Notes
WB: Approx. 40 kDa band observed in human lung lysates (calculated MW of 37.8 kDa band according to NP_009075.1).
Control
MEL-18 Overexpression Lysate
Publications
Read Publication using NB100-1240.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MEL-18 Antibody

  • MEL-18
  • polycomb group ring finger 2
  • polycomb group RING finger protein 2
  • RING finger protein 110DNA-binding protein Mel-18
  • RNF110MEL18
  • Zinc finger protein 144
  • ZNF144MGC10545

Background

MEL-18 is a transcriptional repressor that binds specifically to the DNA sequence 5'-GACTNGACT-3'. Mel18 has tumor suppressor activity and may play a role in cell proliferation and/or neural cell development. It regulates proliferation of early T progenitor cells by maintaining expression of HES1. Also plays a role in antero-posterior specification of the axial skeleton and negative regulation of the self-renewal activity of hematopoietic stem cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MEL-18 Antibody (NB100-1240)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PCGF2
Entrez
Uniprot