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RYBP/DEDAF Antibody (OTI1B2)

Images

 
Western Blot: RYBP/DEDAF Antibody (1B2) [NBP2-02009] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RYBP/DEDAF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Immunocytochemistry/ Immunofluorescence: RYBP/DEDAF Antibody (1B2) [NBP2-02009] Staining of COS7 cells transiently transfected by pCMV6-ENTRY RYBP/DEDAF.
Immunohistochemistry-Paraffin: RYBP/DEDAF Antibody (1B2) [NBP2-02009] - Staining of paraffin-embedded Human pancreas tissue using anti-RYBP/DEDAF mouse monoclonal antibody.
Immunohistochemistry-Paraffin: RYBP/DEDAF Antibody (1B2) [NBP2-02009] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RYBP/DEDAF mouse monoclonal antibody.
Immunohistochemistry-Paraffin: RYBP/DEDAF Antibody (1B2) [NBP2-02009] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RYBP/DEDAF mouse monoclonal antibody.
Immunohistochemistry-Paraffin: RYBP/DEDAF Antibody (1B2) [NBP2-02009] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-RYBP/DEDAF mouse monoclonal antibody.
Immunohistochemistry-Paraffin: RYBP/DEDAF Antibody (1B2) [NBP2-02009] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-RYBP/DEDAF mouse monoclonal antibody.
Immunohistochemistry-Paraffin: RYBP/DEDAF Antibody (1B2) [NBP2-02009] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-RYBP/DEDAF mouse monoclonal antibody.
Immunohistochemistry-Paraffin: RYBP/DEDAF Antibody (1B2) [NBP2-02009] - Staining of paraffin-embedded Human colon tissue using anti-RYBP/DEDAF mouse monoclonal antibody.
Immunohistochemistry-Paraffin: RYBP/DEDAF Antibody (1B2) [NBP2-02009] - Staining of paraffin-embedded Human liver tissue using anti-RYBP/DEDAF mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI1B2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

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RYBP/DEDAF Antibody (OTI1B2) Summary

Immunogen
Full length human recombinant protein of human RYBP(NP_036366) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RYBP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
Theoretical MW
24.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RYBP/DEDAF Antibody (OTI1B2)

  • AAP1
  • APAP-1
  • Apoptin-associating protein 1
  • Death effector domain-associated factor
  • DEDAFYY1 and E4TF1-associated factor 1
  • RING1 and YY1 binding protein
  • RING1 and YY1-binding protein
  • ring1 interactor RYBP
  • YEAF1DED-associated factor
  • YY1 and E4TF1 associated factor 1

Background

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. Several molecules including caspases and adaptor FADD contain DEDs. A novel protein that interacts with DED of caspase-8 and

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol RYBP
Entrez