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HIPPI Antibody (OTI4F6)

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Western Blot: HIPPI Antibody (4F6) [NBP2-02313] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HIPPI (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HIPPI.
Western Blot: HIPPI Antibody (4F6) [NBP2-02313] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HIPPI (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunocytochemistry/ Immunofluorescence: HIPPI Antibody (4F6) [NBP2-02313] Staining of COS7 cells transiently transfected by pCMV6-ENTRY HIPPI.
Immunohistochemistry-Paraffin: HIPPI Antibody (4F6) [NBP2-02313] - Staining of paraffin-embedded Kidney tissue using anti-HIPPI mouse monoclonal antibody.
Immunohistochemistry-Paraffin: HIPPI Antibody (4F6) [NBP2-02313] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-HIPPI mouse monoclonal antibody.
Immunohistochemistry-Paraffin: HIPPI Antibody (4F6) [NBP2-02313] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-HIPPI mouse monoclonal antibody.
Immunohistochemistry-Paraffin: HIPPI Antibody (4F6) [NBP2-02313] - Staining of paraffin-embedded breast tissue using anti-HIPPI mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI4F6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-71985AF350
Alexa Fluor 405
NBP2-71985AF405
Alexa Fluor 488
NBP2-71985AF488
Alexa Fluor 532
NBP2-71985AF532
Alexa Fluor 594
NBP2-71985AF594
Alexa Fluor 647
NBP2-71985AF647
Alexa Fluor 700
NBP2-71985AF700
Alexa Fluor 750
NBP2-71985AF750
Allophycocyanin
NBP2-71985APC
Biotin
NBP2-71985B
CoraFluor 1
NBP2-71985CL1
DyLight 350
NBP2-71985UV
DyLight 405
NBP2-71985V
DyLight 488
NBP2-71985G
DyLight 550
NBP2-71985R
DyLight 594
NBP2-71985DL594
DyLight 650
NBP2-71985C
DyLight 680
NBP2-71985FR
DyLight 755
NBP2-71985IR
FITC
NBP2-71985F
HRP
NBP2-71985H
Janelia Fluor 525
NBP2-71985JF525
Janelia Fluor 549
NBP2-71985JF549
Janelia Fluor 646
NBP2-71985JF646
Janelia Fluor 669
NBP2-71985JF669
PE
NBP2-71985PE
PerCP
NBP2-71985PCP
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-71985
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HIPPI Antibody (OTI4F6) Summary

Immunogen
Full length human recombinant protein of human IFT57(NP_060480) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IFT57
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:2000
Theoretical MW
49.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HIPPI Antibody (OTI4F6)

  • Dermal papilla-derived protein 8
  • DERP8
  • ESRRBL1huntingtin interacting protein-1 interacting protein
  • estrogen-related receptor beta like 1
  • Estrogen-related receptor beta-like protein 1
  • FLJ10147
  • HIPPIHIP1-interacting protein
  • intraflagellar transport 57 homolog (Chlamydomonas)
  • intraflagellar transport protein 57 homolog
  • MHS4R2HIP1 protein interactor

Background

Required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway (By similarity). Has pro-apoptotic function via its interaction with HIP1,leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3'present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator; however the relevance of such function remains unclear

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Bioinformatics

Gene Symbol IFT57
Entrez