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ELAC2 Antibody

Images

 
Western Blot: ELAC2 Antibody [NBP1-84620] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: ELAC2 Antibody [NBP1-84620] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ELAC2 Antibody [NBP1-84620] - Staining of human testis shows strong nuclear and nucleolar positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: ELAC2 Antibody [NBP1-84620] - Staining of human fallopian tube shows weak to moderate nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: ELAC2 Antibody [NBP1-84620] - Staining of human lymph node shows moderate to strong nuclear positivity in germinal center cells.
Immunohistochemistry-Paraffin: ELAC2 Antibody [NBP1-84620] - Staining of human skin shows strong positivity in nucleoli in squamous epithelial cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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ELAC2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: WMERFGPDTQHLVLNENCASVHNLRSHKIQTQLNLIHPDIFPLLTSFRCKKEGPTLSVPMVQGECLLKYQLRPRREWQRDAIITCNPEEFIVEALQLPNFQQSVQEYR
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ELAC2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
ELAC2 Protein (NBP1-84620PEP)
Publications
Read Publication using
NBP1-84620 in the following applications:

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ELAC2 Antibody

  • EC 3.1.26.11
  • elaC homolog 2 (E. coli)
  • ElaC homolog protein 2
  • ELC2
  • FLJ10530
  • FLJ36693
  • FLJ42848
  • Heredity prostate cancer protein 2
  • HPC2elaC (E. coli) homolog 2
  • putative prostate cancer susceptibility protein HPC2/ELAC2
  • Ribonuclease Z 2
  • RNase Z 2
  • tRNA 3 endonuclease 2
  • tRNase Z (long form)
  • tRNase Z 2
  • zinc phosphodiesterase ELAC protein 2

Background

The protein encoded by the ELAC2 gene has a C-terminal domain with tRNA 3′ processing endoribonuclease activity,which catalyzes the removal of the 3' trailer from precursor tRNAs. The protein also interacts with activatedSmad family member 2 (Smad2) and its nuclear partner forkhead box H1 (also known as FAST-1), and reduced expressioncan suppress transforming growth factor-beta induced growth arrest. Mutations in this gene result in an increased riskof prostate cancer. Multiple transcript variants encoding different isoforms have been found for this gene. (providedby RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ELAC2 Antibody (NBP1-84620)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


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IHC-P
(1)
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Human
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ELAC2 Antibody (NBP1-84620) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ELAC2