![Immunohistochemistry-Paraffin: Ret Antibody (RET/2795) - Azide and BSA Free [NBP2-79897] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with RET Mouse Monoclonal Antibody (RET/2795).](http://images.novusbio.com/fullsize/Ret-Antibody-RET-2795-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-79897-img0004.jpg "Immunohistochemistry-Paraffin: Ret Antibody (RET/2795) - Azide and BSA Free [NBP2-79897] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with RET Mouse Monoclonal Antibody (RET/2795).")
| Reactivity | HuSpecies Glossary |
| Applications | IHC, MA |
| Clone | RET/2795 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-79762). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant fragment (around aa 702-848) of human Ret protein (exact sequence is proprietary) (Uniprot: P07949) |
| Localization | Cytoplasmic, Cell Surface, Nuclear |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | RET |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | RET |
![Immunohistochemistry-Paraffin: Ret Antibody (RET/2795) - Azide and BSA Free [NBP2-79897] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with RET Mouse Monoclonal Antibody (RET/2795).](http://images.novusbio.com/fullsize/Ret-Antibody-RET-2795-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-79897-img0002.jpg "Immunohistochemistry-Paraffin: Ret Antibody (RET/2795) - Azide and BSA Free [NBP2-79897] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with RET Mouse Monoclonal Antibody (RET/2795).")
![Immunohistochemistry-Paraffin: Ret Antibody (RET/2795) - Azide and BSA Free [NBP2-79897] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with RET Mouse Monoclonal Antibody (RET/2795).](http://images.novusbio.com/fullsize/Ret-Antibody-RET-2795-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-79897-img0003.jpg "Immunohistochemistry-Paraffin: Ret Antibody (RET/2795) - Azide and BSA Free [NBP2-79897] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with RET Mouse Monoclonal Antibody (RET/2795).")
![Immunohistochemistry-Paraffin: Ret Antibody (RET/2795) - Azide and BSA Free [NBP2-79897] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with RET Mouse Monoclonal Antibody (RET/2795).](http://images.novusbio.com/fullsize/Ret-Antibody-RET-2795-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-79897-img0005.jpg "Immunohistochemistry-Paraffin: Ret Antibody (RET/2795) - Azide and BSA Free [NBP2-79897] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with RET Mouse Monoclonal Antibody (RET/2795).")
![Protein Array: Ret Antibody (RET/2795) - Azide and BSA Free [NBP2-79897] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Ret Antibody (RET/2795). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Ret-Antibody-RET-2795-Azide-and-BSA-Free-Protein-Array-NBP2-79897-img0001.jpg "Protein Array: Ret Antibody (RET/2795) - Azide and BSA Free [NBP2-79897] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Ret Antibody (RET/2795). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.")
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