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Recombinant Mouse CXCL3/DCIP-1 Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
5568-CA-025
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Recombinant Mouse CXCL3/DCIP-1 Protein, CF Summary

Details of Functionality
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR2. The ED50 for this effect is 25-100 ng/mL.
Source
E. coli-derived mouse CXCL3/GRO gamma/CINC-2/DCIP-1 protein
Ala28-Ser100
Accession #
N-terminal Sequence
Ala28
Protein/Peptide Type
Recombinant Proteins
Gene
Cxcl3
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
7.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
6.5 kDa, reducing conditions
Publications
Read Publication using
5568-CA/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CXCL3/DCIP-1 Protein, CF

  • chemokine (C-X-C motif) ligand 3
  • CINC-2
  • CINC-2b
  • C-X-C motif chemokine 3
  • CXCL3
  • Dcip1
  • Gm1960
  • GRO gamma
  • GRO3 oncogene
  • GRO3
  • GROG
  • GRO-gamma
  • GRO-gamma(1-73)
  • Growth-regulated protein gamma
  • Macrophage inflammatory protein 2-beta
  • melanoma growth stimulatory activity gamma
  • MGSA gamma
  • MIP2B
  • MIP-2b
  • MIP2-beta
  • SCYB3

Background

CXCL3 is also known as MIP-2 beta (macrophage inflammatory protein 2 beta), or DCIP-1 (dendritic cell inflammatory protein-1) in mouse, CINC2 (cytokine-induced neutrophil attractant 2) in rat, and GRO-gamma (growth-regulated oncogene gamma) in humans (1, 2). It is an 8 kDa proinflammatory member of the CXC subfamily of heparin-binding chemokines, also called alpha chemokines (1 - 4). The Glu-Leu-Arg (ELR) motif near the CXCL3 N-terminus confers angiogenic properties and distinguishes it from interferon-inducible ELR- CXC chemokines, which are angiostatic (4). ELR+ and ELR- chemokines use CXCR2 and CXCR3 receptors, respectively (3, 4). Mature mouse CXCL3 shares 88% and 57% amino acid (aa) sequence identity with rat and human CXCL3, respectively. Among mouse ELR+ chemokines, it shares 82% aa sequence identity with CXCL2/GRO-beta /MIP-2 and 34% - 58% with CXCL1/GRO-alpha /KC, CXCL5/ENA-78 and CXCL7/NAP-2. Due to their similar sequence and activity, CXCL2 and CXCL3 are sometimes referred to collectively as CXCL2/3, but are separate gene products (4 - 6). Mouse CXCL3 expression is induced in macrophages and early in maturation of DC by bacterial products such as lipopolysaccharides, and other inflammatory mediators (1, 7). It is chemotactic for CXCR2-expressing neutrophils, helping to recruit them to areas of inflammation (1, 7). ELR+ chemokines also elicit endothelial cell chemotaxis, stimulating angiogenesis and playing a role in tumor development (3, 4). ELR+ chemokines upregulated by ischemia play a role in ischemia-reperfusion injury (5, 6). A decoy receptor, DARC (Duffy antigen receptor for chemokines) competes with CXCR2 for ELR+ chemokine binding, thus downregulating their effect (8). Neutrophil influx may also be downregulated by MMP-12, which has been found to inactivate CXCL3 and other ELR+ chemokines by cleaving them at the ELR site (9).

  1. Nolan, K. F. et al. (2004) J. Immunol. 172:2201.
  2. Modi, W. S. and T. Yoshimura (1999) Mol. Biol. Evol. 16:180.
  3. Vandercappellen, J. et al. (2008) Cancer Lett. 267:226.
  4. Strieter, R.M. et al. (2005) Cytokine Growth Factor Rev. 16:593.
  5. Nesmelova, I. V. et al. (2008) J. Biol. Chem. 283:24155.
  6. Maheshwari, A. et al. (2004) Fetal Pediatr. Pathol. 23:145.
  7. Furuichi, K. et al. (2008) Front. Biosci. 13:4021.
  8. Takano, K. and H. Nakagawa (2001) Inflamm. Res. 50:503.
  9. Horton, L. W. et al. (2007) Cancer Res. 67:9791.

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Recombinant Mouse
CXCL3/DCIP-1 Protein, ...
5568-CA/CF
Recombinant Mouse CXCL3/DCIP-1 Protein, CF
Species: Mu
Applications: Bioactivity

Publications for CXCL3/GRO gamma/CINC-2/DCIP-1 (5568-CA/CF)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: In Vivo.


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Bioinformatics

Gene Symbol Cxcl3
Uniprot