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Recombinant Human TGF-beta RII Protein, CF

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Recombinant human TGF-beta RII (241-R2/CF) inhibits recombinant human TGF-beta 1 activity in the HT-2 mouse T cell line. The ED50 for this effect is 1-3 µg/mL in the presence of 0.25 ng/mL of recombinant human TGF-beta 1.
Recombinant human TGF-beta RII (241-R2/CF) inhibits recombinant human TGF-beta 1 activity in the HT-2 mouse T cell line. The ED50 for this effect is 1-3 µg/mL in the presence of 0.25 ng/mL of recombinant human ...read more

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human TGF-beta RII Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit TGF-beta 1 activity on HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is 1-3 µg/mL in the presence of 0.25 ng/mL of recombinant human TGF-beta 1.
Source
Mouse myeloma cell line, NS0-derived human TGF-beta RII protein
Ile24-Asp159
Accession #
N-terminal Sequence
Ile24
Protein/Peptide Type
Recombinant Proteins
Gene
TGFBR2
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
Multiple bands between 20-35 kDa, reducing conditions
Publications
Read Publications using
241-R2/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TGF-beta RII Protein, CF

  • AAT3
  • EC 2.7.11
  • EC 2.7.11.30
  • FAA3
  • HNPCC6
  • LDS1B
  • LDS2B
  • MFS2
  • RIIC
  • TAAD2
  • tbetaR-II
  • TGF-beta receptor type II
  • TGF-beta receptor type IIB
  • TGF-beta receptor type-2
  • TGF-beta RII
  • TGF-beta type II receptor
  • TGFbetaRII
  • TGFbeta-RII
  • TGFBR2
  • TGF-bRII
  • TGFR-2
  • transforming growth factor beta receptor type IIC
  • transforming growth factor, beta receptor II (70/80kDa) isoform 1
  • transforming growth factor, beta receptor II (70/80kDa) isoform 2
  • transforming growth factor, beta receptor II (70/80kDa)
  • transforming growth factor, beta receptor II (70-80kD)
  • Transforming growth factor-beta receptor type II

Background

Most cell types express three sizes of receptors for TGF-beta . These are designated type I (53 kDa), type II (70-85 kDa), and type III (250-350 kDa). The type III receptor, a proteoglycan that exists in membrane-bound and soluble forms, binds TGF-beta 1, TGF-beta 2, and TGF-beta 3 but does not appear to be involved in signal transduction. The type II receptor is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The type I receptor is also a membrane-bound serine/threonine kinase that apparently requires the presence of the type II receptor to bind TGF-beta . Current evidence suggests that signal transduction requires the cytoplasmic domains of both the type I and type II receptors.

The recombinant soluble TGF-beta type II receptor is capable of binding TGF-beta 1, TGF-beta 3, and TGF-beta 5 with sufficient affinity to act as an inhibitor of these isoforms at high concentrations. The soluble receptor also binds TGF-beta 2, but with an affinity at least two orders of magnitude lower. Binding of TGF-beta 1, TGF-beta 3, and TGF-beta 5 to the soluble TGF-beta type II receptor can also be demonstrated by using the soluble receptor as a capture agent on ELISA plates and this observation has been used as the basis for the development of immunoassays for these isoforms of TGF-beta .

  1. Miyazono, K. et al. (1994) Adv. in Immunol. 55:181.

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Recombinant Human
TGF-beta RII Protein, ...
241-R2/CF
Recombinant Human TGF-beta RII Protein, CF
Species: Hu
Applications: Bioactivity

Publications for TGF-beta RII (241-R2/CF)(5)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Bovine.

We have publications tested in 2 applications: Bioassay, IHC.


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Bioassay
(4)
IHC
(1)
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Human
(2)
Mouse
(1)
Rat
(1)
Bovine
(1)
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Showing Publications 1 - 5 of 5.
Publications using 241-R2/CF Applications Species
SX Wang, YN Feng, S Feng, JM Wu, M Zhang, WL Xu, YY Zhang, HB Zhu, H Xiao, ED Dong IMM-H007 attenuates isoprenaline-induced cardiac fibrosis through targeting TGFbeta1 signaling pathway Acta pharmacologica Sinica, 2022-03-30;0(0):. 2022-03-30 [PMID: 35354962] (Bioassay, Mouse) Bioassay Mouse
Graham J, Chamberland A, Lin Q, Li X, Dai D, Zeng W, Ryan M, Rivera-Bermudez M, Flannery C, Yang Z Serine protease HTRA1 antagonizes transforming growth factor-beta signaling by cleaving its receptors and loss of HTRA1 in vivo enhances bone formation. PLoS ONE, 2013-09-11;8(9):e74094. 2013-09-11 [PMID: 24040176] (Bioassay, Human) Bioassay Human
Van Aarsen LA, Leone DR, Ho S, Dolinski BM, McCoon PE, LePage DJ, Kelly R, Heaney G, Rayhorn P, Reid C, Simon KJ, Horan GS, Tao N, Gardner HA, Skelly MM, Gown AM, Thomas GJ, Weinreb PH, Fawell SE, Violette SM Antibody-mediated blockade of integrin alpha v beta 6 inhibits tumor progression in vivo by a transforming growth factor-beta-regulated mechanism. Cancer Res., 2008-01-15;68(2):561-70. 2008-01-15 [PMID: 18199553] (IHC, Human) IHC Human
Fazzini M, Vallejo G, Colman-Lerner A, Trigo R, Campo S, Baranao JL, Saragueta PE Transforming growth factor beta1 regulates follistatin mRNA expression during in vitro bovine granulosa cell differentiation. J. Cell. Physiol., 2006-04-01;207(1):40-8. 2006-04-01 [PMID: 16245315] (Bioassay, Bovine) Bioassay Bovine
Rosado E, 2019, Schwartz Z, Sylvia VL, Dean DD, Boyan BD Transforming growth factor-beta1 regulation of growth zone chondrocytes is mediated by multiple interacting pathways. 91, 2002-06-12;1590(1):1-15. 2002-06-12 [PMID: 12063164] (Bioassay, Rat) Bioassay Rat

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Bioinformatics

Gene Symbol TGFBR2
Uniprot