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Recombinant Human TGF-beta 3 Protein

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As an alternative, please consider our next generation CHO-drived Recombinant Human TGF‑ beta 3 (8420-B3). It has equivalent bioactivity to Sf 21 (baculovirus)-derived Recombinant Human TGF‑ beta 3 (Catalog # ...read more

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human TGF-beta 3 Protein Summary

Details of Functionality
Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is 0.0100-0.0400 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human TGF-beta 3 protein
Ala301-Ser412 (Tyr340Phe)
Accession #
N-terminal Sequence
Ala301
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
TGFB3
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
12.7 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
12 kDa, reducing conditions
24 kDa, non-reducing conditions
Publications
Read Publications using
243-B3 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 20 μg/mL in sterile 4 mM HCl containing 1 mg/mL human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TGF-beta 3 Protein

  • ARVD
  • ARVD1
  • FLJ16571
  • LDS5
  • RNHF
  • TGFB3
  • TGFbeta 3
  • TGF-beta 3
  • TGF-beta3
  • TGF-beta-3
  • transforming growth factor beta-3
  • transforming growth factor, beta 3

Background

TGF­ beta 3 (transforming growth factor-beta 3) is a member of a TGF-beta superfamily subgroup that is defined by their structural and functional similarities (1-5). TGF-beta 3 and its closely related proteins, TGF-beta 1 and ­-beta 2, act as cellular switches to regulate immune function, cell proliferation, and epithelial­-mesenchymal transition (4, 6, 7). The non-redundant biological effects of TGF-beta 3 include involvement in palatogenesis, chondrogenesis, and pulmonary development (1, 2, 7-9). Human TGF-­ beta 3 cDNA encodes a 412 amino acid (aa) precursor that contains a 20 aa signal peptide and a 392 aa proprotein. The proprotein is processed by a furin­like convertase to generate a 220 aa latency­associated peptide (LAP) and a 112 aa mature TGF­-beta 3 (10, 11). Mature human TGF­-beta 3 shows 100%, 99%, and 98% aa identity with mouse/dog/horse, rat, and pig TGF-­ beta 3, respectively. TGF-beta 3 is secreted as a complex with LAP. This latent form of TGF-beta 3 becomes active upon cleavage by plasmin, matrix metalloproteases, thrombospondin-1, and a subset of integrins (12). TGF-beta 3 binds with high affinity to TGF-beta RII, a type II serine/threonine kinase receptor. This receptor then phosphorylates and activates type I serine/threonine kinase receptors, TGF-­ beta RI or ALK-­1, to modulate transcription through Smad phosphorylation (13-15). The divergent biological effects exerted by individual TGF-beta isoforms is dependent upon the recruitment of co-receptors (TGF-­ beta RIII and endoglin) and the subsequent initiation of Smad-­dependent or -independent signaling pathways (14, 16, 17).

  1. Barrio, M.C. et al. (2014) Cells Tissues Organs. [Epub ahead of print; PMID 24861080].
  2. Doetschman, T. et al. (2012) Genesis 50:59.
  3. Mittl, P.R. et al. (1996) Protein Sci. 5:1261.
  4. Sporn, M.B. (2006) Cytokine Growth Factor Rev. 17:3.
  5. Wahl, S.M. et al. (2006) Immunol. Rev. 213:213.
  6. Chang, H. et al. (2002) Endocr. Rev. 23:787.
  7. Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982.
  8. Jin, J.Z. et al. (2014) Dev. Dyn. [Epub ahead of print; PMID 25104574].
  9. Tang, Q.O. et al. (2009) Expert Opin. Biol Ther. 9:689.
  10. Derynck, R. et al. (1988) EMBO J. 7:3737.
  11. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
  12. Oklu, R. and R. Hesketh (2000) Biochem. J. 352 Pt 3:601.
  13. Cui, X.M. and C.F. Shuler (2000) Int. J. Dev. Biol. 44:397.
  14. de Caestecker, M. (2004) Cytokine Growth Factor Rev. 15:1.
  15. Nakajima, A. et al. (2007) Dev. Dyn. 236:791.
  16. Iwata, J. et al. (2012) J. Clin. Invest. 122:873.
  17. Gatza, C.E. et al. (2010) Cell. Signal. 22:1163.

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Publications for TGF-beta 3 (243-B3)(123)

We have publications tested in 13 confirmed species: Human, Mouse, Rat, Bovine, Canine, Drosophila melanogaster, Equine, Horse, Ovine, Primate - Macaca mulatta (Rhesus Macaque), Primate - Rhesus macaque, Rabbit, Xenopus.

We have publications tested in 9 applications: Bioassay, Cell Culture, Differentiation, ELISA (Standard), ICC, In Vivo, In vitro, Surface Plasmon Resonance, Western Blot.


Filter By Application
Bioassay
(103)
Cell Culture
(8)
Differentiation
(4)
ELISA (Standard)
(2)
ICC
(1)
In Vivo
(4)
In vitro
(1)
Surface Plasmon Resonance
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(75)
Mouse
(22)
Rat
(7)
Bovine
(2)
Canine
(4)
Drosophila melanogaster
(1)
Equine
(4)
Horse
(2)
Ovine
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
Primate - Rhesus macaque
(1)
Rabbit
(3)
Xenopus
(1)
All Species
Showing Publications 1 - 10 of 123. Show All 123 Publications.
Publications using 243-B3 Applications Species
Eubanks, E;VanderSleen, K;Mody, J;Patel, N;Sacks, B;Farahani, MD;Wang, J;Elliott, J;Jaber, N;Akçimen, F;Bandres-Ciga, S;Helweh, F;Liu, J;Archakam, S;Kimelman, R;Sharma, B;Socha, P;Guntur, A;Bartels, T;Dettmer, U;Mouradian, MM;Bahrami, AH;Dai, W;Baum, J;Shi, Z;Hardy, J;Kara, E; Increased burden of rare risk variants across gene expression networks predisposes to sporadic Parkinson's disease bioRxiv : the preprint server for biology 2024-09-01 [PMID: 39257816] (Bioassay, Human) Bioassay Human
Saurat, N;Minotti, AP;Rahman, MT;Sikder, T;Zhang, C;Cornacchia, D;Jungverdorben, J;Ciceri, G;Betel, D;Studer, L; Genome-wide CRISPR screen identifies neddylation as a regulator of neuronal aging and AD neurodegeneration Cell stem cell 2024-06-21 [PMID: 38917806] (Bioassay, Human) Bioassay Human
Kojima, R;Paslawski, W;Lyu, G;Arenas, E;Zhang, X;Svenningsson, P; Secretome Analyses Identify FKBP4 as a GBA1-Associated Protein in CSF and iPS Cells from Parkinson's Disease Patients with GBA1 Mutations International journal of molecular sciences 2024-01-04 [PMID: 38203854] (Bioassay, Human) Bioassay Human
Ely, EV;Kapinski, AT;Paradi, SG;Tang, R;Guilak, F;Collins, KH; Designer Fat Cells: Adipogenic Differentiation of CRISPR-Cas9 Genome-Engineered Induced Pluripotent Stem Cells bioRxiv : the preprint server for biology 2023-10-26 [PMID: 37961399] (Bioassay, Mouse) Bioassay Mouse
Aouimeur, I;Sagnial, T;Coulomb, L;Maurin, C;Thomas, J;Forestier, P;Ninotta, S;Perrache, C;Forest, F;Gain, P;Thuret, G;He, Z; Investigating the Role of TGF-? Signaling Pathways in Human Corneal Endothelial Cell Primary Culture Cells 2023-06-14 [PMID: 37371094] (ICC, Human) ICC Human
Z You, L Wang, H He, Z Wu, X Zhang, S Xue, P Xu, Y Hong, M Xiong, W Wei, Y Chen Mapping of clonal lineages across developmental stages in human neural differentiation Cell Stem Cell, 2023-03-17;0(0):. 2023-03-17 [PMID: 36933556] (Bioassay, Human) Bioassay Human
Z You, L Wang, H He, Z Wu, X Zhang, S Xue, P Xu, Y Hong, M Xiong, W Wei, Y Chen Mapping of clonal lineages across developmental stages in human neural differentiation Cell Stem Cell, 2023;0(0):. 2023 [PMID: 36933556] (Bioassay, Human) Bioassay Human
AR Dicks, GI Maksaev, Z Harissa, A Savadipour, R Tang, N Steward, W Liedtke, CG Nichols, CL Wu, F Guilak Skeletal dysplasia-causing TRPV4 mutations suppress the hypertrophic differentiation of human iPSC-derived chondrocytes Elife, 2023-02-22;12(0):. 2023-02-22 [PMID: 36810131] (Bioassay, Human) Bioassay Human
K Santerre, S Cortez Ghi, S Proulx TGF-beta-Mediated Modulation of Cell-Cell Interactions in Postconfluent Maturing Corneal Endothelial Cells Investigative Ophthalmology & Visual Science, 2022-10-03;63(11):3. 2022-10-03 [PMID: 36194422] (Bioassay, Human) Bioassay Human
I Hanson, KE Pitman, U Altanerova, ? Altaner, E Malinen, NFJ Edin Low-Dose-Rate Radiation-Induced Secretion of TGF-beta3 Together with an Activator in Small Extracellular Vesicles Modifies Low-Dose Hyper-Radiosensitivity through ALK1 Binding International Journal of Molecular Sciences, 2022-07-24;23(15):. 2022-07-24 [PMID: 35897723] (Bioassay, Human) Bioassay Human
Show All 123 Publications.

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Bioinformatics

Gene Symbol TGFB3
Uniprot