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Plakophilin 2 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: Plakophilin 2 Antibody [NBP1-86078] - Analysis in human heart muscle and liver tissues using NBP1-86078 antibody. Corresponding Plakophilin 2 RNA-seq data are ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: Plakophilin 2 Antibody [NBP1-86078] - Staining of human heart muscle, liver, prostate and testis using Anti-Plakophilin 2 antibody NBP1-86078 (A) shows ...read more
Orthogonal Strategies: Western Blot: Plakophilin 2 Antibody [NBP1-86078] - Analysis in human cell lines Caco-2 and SK-MEL-30. Corresponding RNA-seq data are presented for the same cell lines. Loading control: ...read more
Immunocytochemistry/ Immunofluorescence: Plakophilin 2 Antibody [NBP1-86078] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cell junctions. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Plakophilin 2 Antibody [NBP1-86078] - Staining of human heart muscle shows strong positivity of intercalated discs.
Immunohistochemistry-Paraffin: Plakophilin 2 Antibody [NBP1-86078] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Plakophilin 2 Antibody [NBP1-86078] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: Plakophilin 2 Antibody [NBP1-86078] - Staining of human prostate shows low positivity in glandular cells as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Plakophilin 2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VGNGNLHRTSSVPEYVYNLHLVENDFVGGRSPVPKTYDMLKAGTTATYEGRWGRGTAQYSSQKSVEERSLRHPLRRLEISPDSSPERAHYTHSDYQYSQRSQAGHTLHHQESRRAALLVPPRY
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PKP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Plakophilin 2 Protein (NBP1-86078PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Plakophilin 2 Antibody

  • ARVD9
  • MGC177501
  • plakophilin 2
  • plakophilin-2

Background

Plakophilins 1, 2, 3 and 4 (PKP1-4) influence development and participate in linking cadherins to cytoskeletal intermediate filaments. Plakophilins 1-4 contain arm-repeat (armadillo) domains and localize to nuclei and cell desmosomes (cell-cell junctions found in suprabasal layers of stratifying epithelia that undergo mechanical stress). Plakophilin 1 mediates increases in desmosomal protein content, desmosome assembly and regulation of cell migration. Plakophilin 2 is important for desmosome assembly and is an essential morphogenic factor and architectural component of the heart. Plakophilin 4 is a component of desmosomal adhesion plaques that regulates junctional plaque organization and cadherin function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PKP2