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![Immunohistochemistry-Paraffin: Plakophilin 2 Antibody [NBP1-86078] - Analysis in human heart muscle and liver tissues using NBP1-86078 antibody. Corresponding Plakophilin 2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Plakophilin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-86078-img0025.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Plakophilin 2 Antibody [NBP1-86078] - Analysis in human heart muscle and liver tissues using NBP1-86078 antibody. Corresponding Plakophilin 2 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Plakophilin 2 Antibody [NBP1-86078] - Staining of human heart muscle, liver, prostate and testis using Anti-Plakophilin 2 antibody NBP1-86078 (A) shows similar protein distribution across tissues to independent antibody NBP2-58748 (B).](http://images.novusbio.com/fullsize/Plakophilin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-86078-img0024.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Plakophilin 2 Antibody [NBP1-86078] - Analysis in human cell lines Caco-2 and SK-MEL-30. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Plakophilin-2-Antibody-Western-Blot-NBP1-86078-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Plakophilin 2 Antibody [NBP1-86078] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cell junctions. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Plakophilin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86078-img0008.jpg)
![Immunohistochemistry-Paraffin: Plakophilin 2 Antibody [NBP1-86078] - Staining of human heart muscle shows strong positivity of intercalated discs.](http://images.novusbio.com/fullsize/Plakophilin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-86078-img0026.jpg)
![Immunohistochemistry-Paraffin: Plakophilin 2 Antibody [NBP1-86078] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Plakophilin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-86078-img0021.jpg)
![Immunohistochemistry-Paraffin: Plakophilin 2 Antibody [NBP1-86078] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Plakophilin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-86078-img0022.jpg)
![Immunohistochemistry-Paraffin: Plakophilin 2 Antibody [NBP1-86078] - Staining of human prostate shows low positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Plakophilin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-86078-img0023.jpg)
![Immunohistochemistry-Paraffin: Plakophilin 2 Antibody [NBP2-58748] - Analysis in human heart muscle and liver tissues using NBP2-58748 antibody. Corresponding Plakophilin 2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Plakophilin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-58748-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Plakophilin 2 Antibody [NBP2-58748] - Analysis in human heart muscle and liver tissues using NBP2-58748 antibody. Corresponding Plakophilin 2 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Plakophilin 2 Antibody [NBP2-58748] - Staining of human heart muscle, liver, prostate and testis using Anti-Plakophilin 2 antibody NBP2-58748 (A) shows similar protein distribution across tissues to independent antibody NBP1-86078 (B).](http://images.novusbio.com/fullsize/Plakophilin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-58748-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Plakophilin 2 Antibody [NBP2-58748] - Staining of human cell line CACO-2 shows localization to cell junctions. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Plakophilin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58748-img0001.jpg)
![Immunohistochemistry-Paraffin: Plakophilin 2 Antibody [NBP2-58748] - Staining of human heart muscle shows strong positivity of intercalated discs.](http://images.novusbio.com/fullsize/Plakophilin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-58748-img0010.jpg)
![Immunohistochemistry-Paraffin: Plakophilin 2 Antibody [NBP2-58748] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Plakophilin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-58748-img0011.jpg)
![Immunohistochemistry-Paraffin: Plakophilin 2 Antibody [NBP2-58748] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Plakophilin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-58748-img0012.jpg)
![Immunohistochemistry-Paraffin: Plakophilin 2 Antibody [NBP2-58748] - Staining of human prostate shows low positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Plakophilin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-58748-img0013.jpg)
![Western Blot: Plakophilin 2 Antibody [NBP1-72054] - (0.3ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Plakophilin-2-Antibody-Western-Blot-NBP1-72054-img0002.jpg "Western Blot: Plakophilin 2 Antibody [NBP1-72054] - (0.3ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Plakophilin 2 Antibody [NBP1-72054] - 3.75ug/ml staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Plakophilin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-72054-img0003.jpg)
![Western Blot: Plakophilin 2 Antibody [NBP2-94257] - Analysis of extracts of various cell lines, using Plakophilin 2 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.](http://images.novusbio.com/fullsize/Plakophilin-2-Antibody-Western-Blot-NBP2-94257-img0001.jpg "Western Blot: Plakophilin 2 Antibody [NBP2-94257] - Analysis of extracts of various cell lines, using Plakophilin 2 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.")
![Western Blot: Plakophilin 2 Overexpression Lysate (Adult Normal) [NBL1-14467] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Plakophilin 2.](http://images.novusbio.com/fullsize/Plakophilin-2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14467-img0002.jpg "Western Blot: Plakophilin 2 Overexpression Lysate (Adult Normal) [NBL1-14467] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Plakophilin 2.")