Recombinant Human His6-USP4 Protein, CF

Name: Recombinant Human His6-USP4 Protein, CF
Brand: Novus Biologicals
SKU: E-596
Price: 363 USD

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Summary

Reactivity	HuSpecies Glossary
Applications	Bioactivity
Format	Carrier-Free

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Recombinant Human His6-USP4 Protein, CF Summary

Details of Functionality	Reaction conditions will need to be optimized for each specific application. We recommend an initial recombinant human USP4 concentration of 10-100 nM when using Ubiquitin-AMC or Ubiquitin-Rh110 substrates (U-550, U-555)
Source	Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP4 protein Met1 - Asn963 with a C-terminal 6-His tag
Accession #	Q13107.3
Protein/Peptide Type	Recombinant Enzymes
Gene	USP4
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Applications/Dilutions

Dilutions	Bioactivity
Theoretical MW	109 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.
Buffer	Supplied as a solution in HEPES, NaCl, TCEP and Glycerol.
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human His6-USP4 Protein, CF

Deubiquitinating enzyme 4
EC 3.1.2.15
EC 3.4.19.12
MGC149848
MGC149849
ubiquitin carboxyl-terminal esterase 4
ubiquitin carboxyl-terminal hydrolase 4
ubiquitin specific peptidase 4 (proto-oncogene)
ubiquitin specific protease 4 (proto-oncogene)
ubiquitin thioesterase 4
Ubiquitin thiolesterase 4
ubiquitin-specific processing protease 4
Ubiquitin-specific-processing protease 4
Ubiquitous nuclear protein homolog
UNP
Unph
UNPUnph
USP4

Background

Ubiquitin carboxyl-terminal hydrolase 4 (USP4) is a specialized cysteine protease with a predicted molecular weight of 109 kDa. USP4 is a member of the peptidase C19 family and the human protein shares 91% amino acid sequence identity with its mouse ortholog. USP4 is ubiquitinated on multiple sites, and deubiquitination of these sites requires USP4 catalytic activity. USP4 interacts with CtIP protein and the MRN (MRE11-RAD50-NBS1) complex to promote DNA double-strand break repair. Other reported USP4 targets include HDAC2, ADORA2A, PDPK1 and TRIM21.

Li, Z., et al. (2016) Oncogene 35:2902.
Milojevic, T., et al. (2006) Mol. Pharmacol. 69:1083.
Wada, K. & T. Kamitani (2006) Biochem. Biophys. Res. Commun. 342:253.
Wijnhoven, P., et al. (2015) Mol. Cell 60:362.

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Recombinant Human
His6-USP4 Protein, CF

E-596

Species: Hu

Applications: Bioactivity

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Bioinformatics

Gene Symbol	USP4
Uniprot	Q13107.3()

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Recombinant Human His6-USP4 Protein, CF

Species Glossary

Images

Product Details

Order Details

Recombinant Human His6-USP4 Protein, CF Summary

Applications/Dilutions

Packaging, Storage & Formulations

Notes

Alternate Names for Recombinant Human His6-USP4 Protein, CF

Background

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Publications for USP4 (E-596) (0)

Reviews for USP4 (E-596) (0)

FAQs for USP4 (E-596) (0)

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Product PDFs

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Bioinformatics