Note: Mouseover a species abbreviation on the product page to display the fullname.
How can we help you?
![Western Blot: USP4 Antibody [NBP2-20834] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with USP4 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/USP4-Antibody-Western-Blot-NBP2-20834-img0004.jpg "Western Blot: USP4 Antibody [NBP2-20834] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with USP4 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: USP4 Antibody [NBP2-20834] - Paraffin-embedded mouse muscle. USP4 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/USP4-Antibody-Immunohistochemistry-Paraffin-NBP2-20834-img0003.jpg)
![Western Blot: USP4 Antibody [NBP2-20834] - Sample (30 ug of whole cell lysate) A: U87-MG 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/USP4-Antibody-Western-Blot-NBP2-20834-img0001.jpg)
![Immunohistochemistry-Paraffin: USP4 Antibody [NBP2-20834] - Immunohistochemical analysis of paraffin-embedded A549 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/USP4-Antibody-Immunohistochemistry-Paraffin-NBP2-20834-img0002.jpg)
![Western Blot: USP4 Antibody [NBP1-86876] - Analysis in human cell line HEL.](http://images.novusbio.com/fullsize/USP4-Antibody-Western-Blot-NBP1-86876-img0010.jpg "Western Blot: USP4 Antibody [NBP1-86876] - Analysis in human cell line HEL.")
![Immunocytochemistry/Immunofluorescence: USP4 Antibody [NBP1-86876] - Staining of human cell line A-431 shows localization to plasma membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/USP4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86876-img0009.jpg)
![Immunohistochemistry-Paraffin: USP4 Antibody [NBP1-86876] - Staining of human rectum shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/USP4-Antibody-Immunohistochemistry-Paraffin-NBP1-86876-img0008.jpg)
![Western Blot: USP4 Antibody [NB100-2869] - Detection of Human and Mouse USP4 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP4 antibody NB100-2869 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/USP4-Antibody-Western-Blot-NB100-2869-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: USP4 Antibody [NB100-2869] - Detection of Human and Mouse USP4 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP4 antibody NB100-2869 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunoprecipitation: USP4 Antibody [NB100-2869] - Detection of human USP4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP4 antibody NB100-2869 used for IP at 6 ug per reaction. USP4 was also immunoprecipitated by rabbit anti-USP4 antibody NB100-2868. For blotting immunoprecipitated USP4, NB100-2869 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/USP4-Antibody-Immunoprecipitation-NB100-2869-img0005.jpg)
![Immunoprecipitation: USP4 Antibody [NB100-2868] - Detection of Human USP4 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-USP4 antibody NB100-2868 used for IP at 3 mcg/mg lysate. USP4 was also immunoprecipitated by rabbit anti-USP4 antibody NB100-2869, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/USP4-Antibody-Immunoprecipitation-NB100-2868-img0002.jpg "<b>Independent Antibodies Validation. </b>Immunoprecipitation: USP4 Antibody [NB100-2868] - Detection of Human USP4 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-USP4 antibody NB100-2868 used for IP at 3 mcg/mg lysate. USP4 was also immunoprecipitated by rabbit anti-USP4 antibody NB100-2869, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: USP4 Overexpression Lysate (Adult Normal) [NBL1-17663] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for USP4.](http://images.novusbio.com/fullsize/USP4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17663-img0002.jpg "Western Blot: USP4 Overexpression Lysate (Adult Normal) [NBL1-17663] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for USP4.")
![Western Blot: USP4 Antibody (5E12) [H00007375-M01] - Analysis of USP4 expression in transfected 293T cell line by USP4 monoclonal antibody (M01), clone 5E12.Lane 1: USP4 transfected lysate (Predicted MW: 108.6 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/USP4-Antibody-5E12-Western-Blot-H00007375-M01-img0001.jpg "Western Blot: USP4 Antibody (5E12) [H00007375-M01] - Analysis of USP4 expression in transfected 293T cell line by USP4 monoclonal antibody (M01), clone 5E12.Lane 1: USP4 transfected lysate (Predicted MW: 108.6 KDa).Lane 2: Non-transfected lysate.")