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Recombinant Human gp130 Fc Chimera Protein, CF

 (11 Publications)
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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human gp130 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit IL-6-dependent proliferation of the 7TD1 mouse hybridoma cells in the presence of Recombinant Human IL-6 R alpha (Catalog # 227-SR). The ED50 for this effect is 0.65-6.5 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human gp130 protein
Human gp130
(Glu23-Ile618)
Accession # P40189		 DIEGRMD Human IgG1
(Pro100-Lys330)		 6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Glu23
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
IL6ST
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
95 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
115-130 kDa, reducing conditions
Publications
Read Publications using
671-GP in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human gp130 Fc Chimera Protein, CF

  • CD130 antigen
  • CD130
  • CDw130
  • DKFZp564F053
  • gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form
  • gp130
  • IL-6 receptor subunit beta
  • IL-6R subunit beta
  • IL-6RB
  • IL-6R-beta
  • IL6ST
  • interleukin 6 signal transducer (gp130, oncostatin M receptor)
  • interleukin receptor beta chain
  • interleukin-6 receptor subunit beta
  • Interleukin-6 signal transducer
  • Membrane glycoprotein 130
  • membrane glycoprotein gp130
  • Oncostatin-M receptor subunit alpha

Background

Gp130, the common signal transducing receptor component shared by the functional receptor complexes of the IL-6 family of cytokines, belongs to the class I cytokine receptor family. Binding of IL-6 (IL-11) to either the membrane-anchored or soluble IL-6 R (IL-11 R) initiates the association of IL-6 R (IL-11 R) with gp130 which then undergoes homo-dimerization and signal transduction. With other IL-6 family cytokines, such as LIF and OSM, signal transduction is triggered by the hetero-dimerization of gp130 and LIF R or OSM R.

Gp130 is expressed in all organs examined. Soluble gp130, which apparently arises either from proteolytic cleavage or the membrane-bound receptor of from alternative splicing, has been detected in human serum. In in vitro experiments, natural or recombinant soluble gp130 has been shown to have inhibitory effects on OSM and CNTF activities.

  1. Narazaki, M. et al. (1993) Blood 82:1120.
  2. Taga, T. and T. Kishimoto (1997) Annu. Rev. Immunol. 15:797.

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Recombinant Human
gp130 Fc Chimera Prot ...
671-GP
Recombinant Human gp130 Fc Chimera Protein, CF
Species: Hu
Applications: Bioactivity

Publications for gp130/CD130 (671-GP)(11)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: Bioassay, Control, ELISA (Capture), Neutralization.


Filter By Application
Bioassay
(7)
Control
(1)
ELISA (Capture)
(1)
Neutralization
(1)
All Applications
Filter By Species
Human
(10)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using 671-GP Applications Species
Rodríguez-Hernández, MÁ;Baena-Bustos, M;Carneros, D;Zurita-Palomo, C;Muñoz-Pinillos, P;Millán, J;Padillo, FJ;Smerdou, C;von Kobbe, C;Rose-John, S;Bustos, M; Targeting IL-6 trans-signalling by sgp130Fc attenuates severity in SARS-CoV-2 -infected mice and reduces endotheliopathy EBioMedicine 2024-04-26 [PMID: 38677182] (Bioassay, Human) Bioassay Human
D Mosly, K MacLeod, N Moir, A Turnbull, AH Sims, SP Langdon Variation in IL6ST cytokine family function and the potential of IL6 trans-signalling in ERalpha positive breast cancer cells Cellular Signalling, 2022-12-21;103(0):110563. 2022-12-21 [PMID: 36565897] (Human) Human
M Lindkvist, MM Zegeye, M Grenegård, LU Ljungberg Pleiotropic, Unique and Shared Responses Elicited by IL-6 Family Cytokines in Human Vascular Endothelial Cells International Journal of Molecular Sciences, 2022-01-27;23(3):. 2022-01-27 [PMID: 35163371] (Bioassay, Human) Bioassay Human
J Dong, S Viswanatha, E Adami, BK Singh, SP Chothani, B Ng, WW Lim, J Zhou, M Tripathi, NSJ Ko, SG Shekeran, J Tan, SY Lim, M Wang, PM Lio, PM Yen, S Schafer, SA Cook, AA Widjaja Hepatocyte-specific IL11 cis-signaling drives lipotoxicity and underlies the transition from NAFLD to NASH Nature Communications, 2021-01-04;12(1):66. 2021-01-04 [PMID: 33397952] (Bioassay, Human) Bioassay Human
R Robinson, D Brown, L Churchwell, TJ Lee, SK Kodeboyina, J Bloom, A Sharma, S Sharma RNA-Seq analysis reveals gene expression changes induced by IL-6 trans-signaling activation in retinal endothelial cells Cytokine, 2020-12-28;139(0):155375. 2020-12-28 [PMID: 33383381] (Neutralization, Human) Neutralization Human
M Werner-Kle, A Grujovic, C Irlbeck, M Obradovi?, M Hoffmann, H Koerkel-Qu, X Lu, S Treitschke, C Köstler, C Botteron, K Weidele, C Werno, B Polzer, S Kirsch, M Gužvi?, J Warfsmann, K Honarnejad, Z Czyz, G Feliciello, I Blochberge, S Grunewald, E Schneider, G Haunschild, N Patwary, S Guetter, S Huber, B Rack, N Harbeck, S Buchholz, P Rümmele, N Heine, S Rose-John, CA Klein Interleukin-6 trans-signaling is a candidate mechanism to drive progression of human DCCs during clinical latency Nat Commun, 2020-10-05;11(1):4977. 2020-10-05 [PMID: 33020483] (Bioassay, Human) Bioassay Human
M Findeisen, TL Allen, DC Henstridge, H Kammoun, AE Brandon, LL Baggio, KI Watt, M Pal, L Cron, E Estevez, C Yang, GM Kowalski, L O'Reilly, C Egan, E Sun, LM Thai, G Krippner, TE Adams, RS Lee, J Grötzinger, C Garbers, S Risis, MJ Kraakman, NA Mellet, J Sligar, ET Kimber, RL Young, MA Cowley, CR Bruce, PJ Meikle, PA Baldock, P Gregorevic, TJ Biden, GJ Cooney, DJ Keating, DJ Drucker, S Rose-John, MA Febbraio Treatment of type 2 diabetes with the designer cytokine IC7Fc Nature, 2019-09-25;574(7776):63-68. 2019-09-25 [PMID: 31554967] (Bioassay, Human) Bioassay Human
Dietrich C, Candon S, Ruemmele F, Devergne O A soluble form of IL-27Ralpha is a natural IL-27 antagonist. J Immunol, 2014-04-25;192(11):5382-9. 2014-04-25 [PMID: 24771852] (Bioassay, Human) Bioassay Human
Chollangi , Srinivas, Mather , Timothy, Rodgers , Karla K, Ash , John D A unique loop structure in oncostatin M determines binding affinity toward oncostatin M receptor and leukemia inhibitory factor receptor. J Biol Chem, 2012-07-24;287(39):32848-59. 2012-07-24 [PMID: 22829597] (ELISA (Capture), Human) ELISA (Capture) Human
Abir R, Fisch B, Jin S, Barnnet M, Freimann S, van den Hurk R, Feldberg D, Nitke S, Krissi H, Ao A Immunocytochemical detection and RT-PCR expression of leukaemia inhibitory factor and its receptor in human fetal and adult ovaries. Mol. Hum. Reprod., 2004-03-25;10(5):313-9. 2004-03-25 [PMID: 15044601] (Control) Control
Show All 11 Publications.

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Bioinformatics

Gene Symbol IL6ST
Uniprot