Recombinant Human GDF-5 (Catalog # 8340-G5) induces alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.2-1.2 μg/mL.
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 0.2-1.2 μg/mL.
Source
E. coli-derived human GDF-5/BMP-14 protein Ala382-Arg501
<0.10 EU per 1 μg of the protein by the LAL method.
Applications/Dilutions
Dilutions
Bioactivity
Theoretical MW
14 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
14 kDa, reducing conditions
Publications
Read Publications using 8340-G5 in the following applications:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in HCl.
Purity
>90%, by SDS-PAGE with silver staining.
Reconstitution Instructions
Reconstitute at 250 μg/mL in 4 mM HCl.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Human GDF-5 Protein, CF
BMP14
BMP-14
Cartilage-derived morphogenetic protein 1
cartilage-derived morphogenetic protein-1
CDMP-1
CDMP1LAP4
GDF5
GDF-5
growth differentiation factor 5
growth/differentiation factor 5
OS5
radotermin
SYNS2
Background
Growth Differentiation Factor-5 (GDF-5; also called BMP-14 and CDMP-1) is a member of the BMP family of TGF-beta superfamily proteins (1, 2). Human GDF-5, -6, and -7 are a defined subgroup of the BMP family (3). GDF-5 is synthesized as a homodimeric precursor protein consisting of a 354 amino acid (aa) N-terminal pro-region and a 120 aa C-terminal mature peptide. Mature human GDF-5 shares 99% aa sequence identity with both mature mouse and rat GDF-5. GDF-5 signaling is mediated by formation of a heterodimeric complex consisting of a type 1 (BMPR-IB) and a type II (BMPR-II or Activin RII) serine/threonine kinase receptor which results in the phosphorylation and activation of cytosolic Smad proteins (Smad1, 5, and 8) (4, 5). Similar to other BMP family proteins, GDF-5 signaling is antagonized by Noggin (6). GDF-5 is involved in multiple developmental processes including limb generation, cartilage development, joint formation, bone morphogenesis, cell survival, and neuritogenesis (7-11). Exogenous GDF-5 has been reported to promote chondrogenesis, osteogenesis, and angiogenesis in mesenchymal stem cells in vivo and in vitro (12-14). Inhibition of GDF-5 expression or alteration of its signaling can facilitate the development of osteoarthritis (15-18).
Storm, E.E. et al. (1994) Nature 368:639.
Hotten, G. et al. (1994) Biochem. Biophys. Res. Commun. 204:646.
Mikic, B. (2004) Ann. Biomed. Eng. 32:466.
Nishitoh, H. et al. (1996) J. Biol. Chem. 271:21345.
Mueller, T.D. and J. Nickel (2012) FEBS Lett. 586:1846.
Bragdon, B. et al. (2011) Cell. Signal. 23:609.
Sieber, C. et al. (2006) Biol. Chem. 387:451.
Osorio, C. et al. (2013) Development 140:4751.
Wood, T.K. et al. (2005) J. Neurosci. Res. 80:759.
Francis-West, P.H. et al. (1999) Development 126:1305.
Brunet, L.J. et al. (1998) Science 280:1455.
Zeng, Q. et al. (2007) Bone 40:374.
Coleman, C.M. et al. (2013) Stem Cells Dev. 22:1968.
Cheng, X. et al. (2012) Cells Tissues Organs 196:56.
Zhang, Y. et al. (2014) Exp. Mol. Med. 46:e79.
Miyamoto, Y. et al. (2007) Nat. Genet. 39:529.
Vaes, R.B. et al. (2009) Ann. Rheum. Dis. 68:1754.
Degenkolbe, E. et al. (2013) PLoS Genet. 9:e1003846.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
=
÷
Review this Product
Be the first to review our Recombinant Human GDF-5 Protein, CF and receive a gift card or discount.
![Bioactivity BMP-2 [Unconjugated]](https://images.novusbio.com/images/protein/355-bm_recombinant-human-mouse-rat-bmp-2-protein-bioactivity-181120209742.jpg)
![Bioactivity BMP-7 [Unconjugated]](https://images.novusbio.com/images/protein/354-bp_recombinant-human-bmp-7-protein-bioactivity-7122020141940.jpg)
![Cell Culture BMP-7 [Unconjugated]](https://images.novusbio.com/images/354-bp_recombinant-human-bmp-7-protein-cell-culture-252023113257..jpg)
![SDS-PAGE BMP-4 [Unconjugated]](https://images.novusbio.com/images2/BMP-4_314-BP_100.jpg)
![Bioactivity BMP-4 [Unconjugated]](https://images.novusbio.com/images2/314-bp_recombinant-human-bmp-4-protein-1882021123744.jpg)
![SDS-PAGE BMP-6 [Unconjugated]](https://images.novusbio.com/images2/BMP-6_507-BP_374.jpg)
![Bioactivity BMP-6 [Unconjugated]](https://images.novusbio.com/images2/BMP-6_507-BP_376.jpg)
![Bioactivity Noggin [Unconjugated]](https://images.novusbio.com/images2/Noggin_6057NG_2865.jpg)
![Bioactivity Noggin [Unconjugated]](https://images.novusbio.com/images2/Noggin_6057NG_923.jpg)
![Bioactivity Noggin [Unconjugated]](https://images.novusbio.com/images2/6057-ng_recombinant-human-noggin-protein-bioactivity-1022023121039.jpg)
![Flow Cytometry BMPR-II Antibody [Unconjugated]](https://images.novusbio.com/images2/BMPRII_AF811_Flow_Cytometry_15364.jpg)
![Immunohistochemistry BMPR-II Antibody [Unconjugated]](https://images.novusbio.com/images2/BMPR-II_AF811_Immunohistochemistry_7345.jpg)
![Bioactivity GDF-7/BMP-12 [Unconjugated]](https://images.novusbio.com/images2/GDF7_8386G7_1029.jpg)
![Western Blot BMPR-IA/ALK-3 Antibody [Unconjugated]](https://images.novusbio.com/images2/BMPR-IA_AF346_Western_Blot_9327.jpg)
![Immunohistochemistry BMPR-IA/ALK-3 Antibody [Unconjugated]](https://images.novusbio.com/images2/af346_human-bmpr-ia-alk-3-affinity-purified-polyclonal-ab-immunohistochemistry-35202018211.jpg)
![Immunocytochemistry SOX9 Antibody [Unconjugated]](https://images.novusbio.com/images2/SOX9_AF3075_Immunocytochemistry_15995.jpg)
![Western Blot SOX9 Antibody [Unconjugated]](https://images.novusbio.com/images2/SOX9_AF3075_Western_Blot_21580.jpg)
![Immunocytochemistry SOX9 Antibody [Unconjugated]](https://images.novusbio.com/images2/SOX9_AF3075_Immunocytochemistry_6490.jpg)
![N/A SLPI [HRP]](https://images.novusbio.com/images2/DATA_SLPI_DPI00_ELISA_792.jpg)
![N/A SLPI [HRP]](https://images.novusbio.com/images2/SLPI_DPI00_ELISA_160.jpg)
![Bioactivity TGF-beta 1 [Unconjugated]](https://images.novusbio.com/images/protein/7754-bhcf_recombinant-human-tgf-beta-1-human-cell-expressed-cf-bioactivity-1811202013946.jpg)