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Recombinant Human CD68/SR-D1 His-tag Protein, CF

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2 μg/lane of Recombinant Human CD68/SR-D1 His-tag Protein (Catalog # 10551-SR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing broad diffusive bands centered at 70-100 kDa.
2 μg/lane of Recombinant Human CD68/SR-D1 His-tag Protein (Catalog # 10551-SR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing ...read more

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human CD68/SR-D1 His-tag Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Human S100A8 (Catalog # 9876-S8) is immobilized at 10 µg/mL (100 µL/well), it binds to Recombinant Human CD68/SR‑D1 His-tag with an ED50 of 5-30 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human CD68/SR-D1 protein
Asn22-Ser319, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Asn22
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
70-100 kDa, under reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CD68/SR-D1 His-tag Protein, CF

  • CD_antigen: CD68
  • CD68 antigen
  • CD68 molecule
  • CD68
  • DKFZp686M18236
  • gp110
  • LAMP4
  • macrophage antigen CD68
  • Macrosialin
  • SCARD1
  • scavenger receptor class D, member 1
  • SRD1
  • SR-D1

Background

CD68, also called Scavenger Receptor D1 (SR-D1) and LAMP-4, is a heavily glycosylated type I transmembrane glycoprotein that belongs to the LAMP family comprised of five lysosome-specific protein members including LAMP-1, LAMP-2, dendritic cell (DC)-LAMP (also known as CD208 and LAMP-3), and brain and dendritic cell-associated (BAD)-LAMP (also known as LAMP-5) (1, 2). CD68 expression represents the classical immunohistology marker molecule for human monocytes and tissue resident macrophages (3). It is mainly associated with endosomal/lysosomal compartment and serves as a scavenger receptor for oxidized LDL (4). In contrast to other members of the LAMP family of glycoproteins which are ubiquitously expressed in all cell types, CD68 appears to have a more restricted, cell-type specific pattern of expression (5). Human CD68 is expressed as a 354 amino acid (aa) protein, which includes a 21 aa signal peptide, a 298 aa extracellular domain (ECD), a 25 aa transmembrane segment, and a 10 aa cytoplasmic domain. Within the ECD, human CD68 shares 65% and 67% sequence identity with mouse and rat CD68, respectively. CD68 is a biomarker of rheumatoid arthritis and Hodgkin's lymphoma (6,7). In addition, CD68 on macrophages binds tightly to both S100A8 and S100A9 to enhance the cell immunity and influences the functions of cells through NF-kB/focal adhesion kinase pathway (8, 9).
  1. Chistiakov, D. et al. (2016) Lab. Invest. 97:4.
  2. Holness, C.L. and Simmons, D.L. (1993) Blood 81:1607.
  3. O’Reilly D. et al. (2007) Genomics 90:3.
  4. Ramprasad, M.P. et al. (1996) Proc. Natl. Acad. Sci. U. S. A. 93:14838.
  5. Gottfried, E. et al. (2008) Scand. J. Immunol. 67:453.
  6. Bresnihan, B. et al. (2009) J. Rheumatol. 36:1800.
  7. Touati, M. et al. (2015) Leuk. Lymphoma 56:332.
  8. Wang, H. et al. (2014) Cancer Lett. 345:91.
  9. Okada, K. et al. (2016) J. Leukoc. Biol. 100:1093.

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