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Rab5a Antibody (3A4) - BSA Free

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Western Blot: Rab5a Antibody (3A4) [NBP1-04340] - The extracts of mouse brain (45ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Rab5a (1:500). Proteins were visualized using a goat ...read more
Flow Cytometry: Mouse Rab5a mAb (3A4) - BSA Free [NBP1-04340] - Flow cytometry of purified endosomes from mouse bone marrow derived dendritic cells bearing (blue curve) or absent of (red curve) fluorescent antigen. ...read more
HAP1 lysates were prepared, and immunoprecipitation was performed using 2.0 ug of Rab5a antibody (Catalog # NBP1-04340) pre-coupled to Dynabeads protein G. Samples were washed and processed for Western Blot with Rab5a ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IP
Clone
3A4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

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Rab5a Antibody (3A4) - BSA Free Summary

Immunogen
Recombinant human Rab5a (1-215aa) purified from E. coli
Marker
Early Endosome Marker
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
RAB5A
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Flow Cytometry Validated for Flow Cytometry from a verified customer review.
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Western Blot 1:500-1:2000
Publications
Read Publication using NBP1-04340.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Rab5a Antibody (3A4) - BSA Free

  • RAB5A, member RAS oncogene family
  • RAB5RAS-associated protein RAB5A
  • ras-related protein Rab-5A

Background

Rab5a is a member of the RAS oncogene family. Rab5a is a small GTPase localized to the plasma membrane and early endosomes. It is a crucial regulatory component of the endocytic pathway. Activation of Rab5a is mediated by GDP-GTP exchange factors (GEFs) that generate the Rab5-GTP complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Rab5a Antibody (NBP1-04340)(1)


Showing Publication 1 - 1 of 1.
Publication using NBP1-04340 Applications Species
Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Rab5a Antibody (NBP1-04340) (0)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol RAB5A
Uniprot