![Western Blot: PRPF4 Antibody [NBP1-83217] - Analysis using Anti-PRPF4 antibody NBP1-83217 (A) shows similar pattern to independent antibody NBP1-83218 (B).](http://images.novusbio.com/fullsize/PRPF4-Antibody-Western-Blot-NBP1-83217-img0009.jpg "Western Blot: PRPF4 Antibody [NBP1-83217] - Analysis using Anti-PRPF4 antibody NBP1-83217 (A) shows similar pattern to independent antibody NBP1-83218 (B).")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: ISYCHFSPNSKMLATACWSGLCKLWSVPDCNLLHTLRGHNTNVGAIVFHPKSTVSLDPKDVNLASCAADGSVKLWSLDSDEPVADIE |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PRPF4 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
||
| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100. |
||
| Control Peptide |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | PRPF4 |
![Immunocytochemistry/Immunofluorescence: PRPF4 Antibody [NBP1-83217] - Staining of human cell line A-431 shows localization to nuclear speckles.](http://images.novusbio.com/fullsize/PRPF4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83217-img0007.jpg "Immunocytochemistry/Immunofluorescence: PRPF4 Antibody [NBP1-83217] - Staining of human cell line A-431 shows localization to nuclear speckles.")
![Immunohistochemistry-Paraffin: PRPF4 Antibody [NBP1-83217] - Staining of human lymph node.](http://images.novusbio.com/fullsize/PRPF4-Antibody-Immunohistochemistry-Paraffin-NBP1-83217-img0012.jpg "Immunohistochemistry-Paraffin: PRPF4 Antibody [NBP1-83217] - Staining of human lymph node.")
![Western Blot: PRPF4 Antibody [NBP1-83217] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG](http://images.novusbio.com/fullsize/PRPF4-Antibody-Western-Blot-NBP1-83217-img0005.jpg "Western Blot: PRPF4 Antibody [NBP1-83217] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG")
![Western Blot: PRPF4 Antibody [NBP1-83217] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/PRPF4-Antibody-Western-Blot-NBP1-83217-img0006.jpg "Western Blot: PRPF4 Antibody [NBP1-83217] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).")
![Immunohistochemistry-Paraffin: PRPF4 Antibody [NBP1-83217] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PRPF4-Antibody-Immunohistochemistry-Paraffin-NBP1-83217-img0002.jpg "Immunohistochemistry-Paraffin: PRPF4 Antibody [NBP1-83217] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: PRPF4 Antibody [NBP1-83217] - Staining of human cerebral cortex, liver, lymph node and testis using Anti-PRPF4 antibody NBP1-83217 (A) shows similar protein distribution across tissues to independent antibody NBP1-83218 (B).](http://images.novusbio.com/fullsize/PRPF4-Antibody-Immunohistochemistry-Paraffin-NBP1-83217-img0008.jpg "Immunohistochemistry-Paraffin: PRPF4 Antibody [NBP1-83217] - Staining of human cerebral cortex, liver, lymph node and testis using Anti-PRPF4 antibody NBP1-83217 (A) shows similar protein distribution across tissues to independent antibody NBP1-83218 (B).")
![Immunohistochemistry-Paraffin: PRPF4 Antibody [NBP1-83217] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/PRPF4-Antibody-Immunohistochemistry-Paraffin-NBP1-83217-img0010.jpg "Immunohistochemistry-Paraffin: PRPF4 Antibody [NBP1-83217] - Staining of human cerebral cortex.")
![Immunohistochemistry-Paraffin: PRPF4 Antibody [NBP1-83217] - Staining of human liver.](http://images.novusbio.com/fullsize/PRPF4-Antibody-Immunohistochemistry-Paraffin-NBP1-83217-img0011.jpg "Immunohistochemistry-Paraffin: PRPF4 Antibody [NBP1-83217] - Staining of human liver.")
![Immunohistochemistry-Paraffin: PRPF4 Antibody [NBP1-83217] - Staining of human testis.](http://images.novusbio.com/fullsize/PRPF4-Antibody-Immunohistochemistry-Paraffin-NBP1-83217-img0013.jpg "Immunohistochemistry-Paraffin: PRPF4 Antibody [NBP1-83217] - Staining of human testis.")
![Western Blot Brg1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Brg1_AF5738_Western_Blot_5829.jpg)
![Immunocytochemistry Brg1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Brg1_AF5738_Immunocytochemistry_11663.jpg)
![Immunocytochemistry Brg1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Brg1_AF5738_Immunocytochemistry_11664.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")