Home » Protogenin » Protogenin Antibodies » Protogenin Antibody [Unconjugated]
Novus Biologicals products are now on bio-techne.com

Protogenin Antibody [Unconjugated]

 Submit a Review

Images

 
(
Enlarge)
Protogenin was detected in immersion fixed frozen sections of embryonic mouse neural tube (E9) using 10 µg/mL Sheep Anti-Mouse Protogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4919) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Protogenin was detected in immersion fixed frozen sections of embryonic mouse neural tube (E9) using 10 µg/mL Sheep Anti-Mouse Protogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4919) overnight at 4 ...read more
Protogenin was detected in immersion fixed frozen sections of mouse neural tube (E9.5) using Sheep Anti-Mouse Protogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4919) at 10 µg/mL overnight at 4 °C. ...read more
Protogenin was detected in immersion fixed paraffin-embedded 13.5 d.p.c sections of Mouse Embryo using Sheep Anti-Mouse Protogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4919) at 10 µg/mL ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF4919
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF4919-SP
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Protogenin Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse PRTG
Val24-Thr943
Accession # Q2EY15
Specificity
Detects mouse and human PRTG in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
PRTG
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Protogenin Antibody [Unconjugated]

  • FLJ12795
  • FLJ13221
  • FLJ25756
  • IGDCC5
  • immunoglobulin superfamily, DCC subclass, member 5
  • Protein Shen-Dan
  • protogenin homolog (Gallus gallus)
  • Protogenin
  • PRTG
  • RPTG
  • Shen-Dan

Background

Protogenin (Early/proto in expression, neogenin-like; also Prtg) is a 130 kDa (predicted), type I transmembrane member of the DCC/Neogenin family of proteins. It is expressed in mouse presomitic mesoderm, paraxial mesoderm, and neural tube. Mature mouse protogenin is 1168 amino acids (aa) in length. It contains a 920 aa extracellular domain (ECD) (aa 24-943) plus a 224 aa cytoplasmic region. The ECD contains four consecutive Ig-like domains (aa 24-405), followed by five sequential fibronectin type III domains (aa 413-908). The ECD of mouse protogenin shares 98%, 87%, and 93% aa sequence identity with the ECD of rat, chick, and human protogenin, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

DCC Antibody
[Unconjugated]
AF844
Immunohistochemistry DCC Antibody [Unconjugated]
(1 Review)
(12 Publications)
Species: Mu
Applications: Block, IHC, WB
Neogenin Antibody
[Unconjugated]
AF1079
Immunohistochemistry Neogenin Antibody [Unconjugated]
Western Blot Neogenin Antibody [Unconjugated]
Simple Western Neogenin Antibody [Unconjugated]
(16 Publications)
Species: Hu, Mu
Applications: Block, IHC, Simple Western, WB
BMP-4
[Unconjugated]
314-BP
SDS-PAGE BMP-4 [Unconjugated]
Bioactivity BMP-4 [Unconjugated]
(519 Publications)
Species: Hu
Applications: BA, BA
DYX1C1 Antibody
NBP1-68766
Immunohistochemistry-Paraffin DYX1C1 Antibody
Western Blot DYX1C1 Antibody
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, PEP-ELISA, WB
Nope/IGDCC4
Antibody [Unconjugate
AF1394
Nope/IGDCC4 Antibody [Unconjugated]
(2 Publications)
Species: Mu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), WB
IGDCC3 Antibody
(920038) [Unconjugate
MAB8559
Western Blot IGDCC3 Antibody (920038) [Unconjugated]
Immunocytochemistry IGDCC3 Antibody (920038) [Unconjugated]
Flow Cytometry IGDCC3 Antibody (920038) [Unconjugated]
Species: Hu
Applications: CyTOF-ready, Flow, ICC, WB
p19 INK4d Antibody
(DCS-100)
NBP3-11884
Immunocytochemistry/ Immunofluorescence p19 INK4d Antibody (DCS-100)
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, RIA, WB
IL-23A/IL-23 P19
[Unconjugated]
1290-IL
SEC-MALS IL-23A/IL-23 P19 [Unconjugated]
(115 Publications)
Species: Hu
Applications: BA
PEX7 Antibody
NBP1-57578
Western Blot PEX7 Antibody
(1 Publication)
Species: Hu
Applications: WB
p19ARF/CDKN2A
Antibody - BSA Free
NB200-106
Immunohistochemistry p19ARF/CDKN2A Antibody - BSA Free
Western Blot p19ARF/CDKN2A Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence p19ARF/CDKN2A Antibody - BSA Free
(1 Review)
(48 Publications)
Species: Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB
SLC7A5/LAT1
Antibody (BU53) - BSA
NBP2-50465
Immunohistochemistry-Paraffin SLC7A5/LAT1 Antibody (BU53) - BSA Free
Flow (Cell Surface) SLC7A5/LAT1 Antibody (BU53) - BSA Free
Flow (Cell Surface) SLC7A5/LAT1 Antibody (BU53) - BSA Free
(3 Publications)
Species: Hu
Applications: CyTOF-ready, Flow-CS, Flow, IHC, IHC-P
PHYH Antibody
NBP1-87349
Western Blot PHYH Antibody
Western Blot PHYH Antibody
Immunohistochemistry-Paraffin PHYH Antibody
Species: Hu, Mu
Applications: IHC, IHC-P, WB
Reg1A Antibody
(431202) [Unconjugate
MAB4937
Intracellular Staining by Flow Cytometry Reg1A Antibody (431202) [Unconjugated]
(2 Publications)
Species: Hu
Applications: CyTOF-ready, ICFlow, WB
DNAJB11 Antibody
NBP1-84899
Immunohistochemistry-Paraffin DNAJB11 Antibody
Western Blot DNAJB11 Antibody
Western Blot DNAJB11 Antibody
(1 Publication)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
ERMAP Antibody
[Unconjugated]
AF4928
Flow Cytometry ERMAP Antibody [Unconjugated]
Species: Hu
Applications: CyTOF-ready, Flow, WB
Factor IX Antibody
NBP1-33581
Immunohistochemistry-Paraffin Factor IX Antibody
Immunohistochemistry-Paraffin Factor IX Antibody
Immunocytochemistry/ Immunofluorescence Factor IX Antibody
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P
ELSPBP1 Antibody
NBP2-13957
Western Blot ELSPBP1 Antibody
Immunohistochemistry-Paraffin ELSPBP1 Antibody
Immunohistochemistry-Paraffin ELSPBP1 Antibody
Species: Hu
Applications: IHC, IHC-P, WB
Nestin Antibody
(196908) [Unconjugate
MAB1259
Immunocytochemistry Nestin Antibody (196908) [Unconjugated]
Intracellular Staining by Flow Cytometry Nestin Antibody (196908) [Unconjugated]
Western Blot Nestin Antibody (196908) [Unconjugated]
(3 Reviews)
(161 Publications)
Species: Hu
Applications: CyTOF-reported, ICC, ICFlow

Publications for Protogenin Antibody (AF4919) (0)

There are no publications for Protogenin Antibody (AF4919).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Protogenin Antibody (AF4919) (0)

There are no reviews for Protogenin Antibody (AF4919). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Protogenin Antibody (AF4919) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Protogenin Products

Blogs on Protogenin

There are no specific blogs for Protogenin, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Protogenin Antibody [Unconjugated] and receive a gift card or discount.


Bioinformatics

Gene Symbol PRTG
Uniprot