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Protogenin Antibody [Biotin]

Name: Protogenin Antibody [Biotin]
Brand: Novus Biologicals
SKU: BAF4919
Price: 499 USD

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Product Details

Summary

Reactivity	MuSpecies Glossary
Applications	WB
Clonality	Polyclonal
Host	Sheep
Conjugate	Biotin
Concentration	LYOPH

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Catalog# & Conjugate	Size	Price
Unconjugated AF4919

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Catalog# & Formulation	Size	Price
Standard Formulation AF4919

Carrier Free AF4919

Carrier Free AF4919-SP

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Conjugate	Catalog #	Availability	Size	Price
Unconjugated	AF4919

Protogenin Antibody [Biotin] Summary

Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse Protogenin Val24-Thr943 Accession # Q2EY15
Specificity	Detects mouse and human Protogenin in Western blots.
Source	N/A
Isotype	IgG
Clonality	Polyclonal
Host	Sheep
Gene	PRTG
Purity Statement	Antigen Affinity-purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Applications/Dilutions

Dilutions	Western Blot 0.1 ug/mL
Readout System	Streptavidin Native Protein NBP2-29367 Streptavidin Native Protein NBP2-29370 Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Protogenin Antibody [Biotin]

FLJ12795
FLJ13221
FLJ25756
IGDCC5
immunoglobulin superfamily, DCC subclass, member 5
Protein Shen-Dan
protogenin homolog (Gallus gallus)
Protogenin
PRTG
RPTG
Shen-Dan

Background

Protogenin (Early/proto in expression, neogenin-like; also Prtg) is a 130 kDa (predicted), type I transmembrane member of the DCC/Neogenin family of proteins. It is expressed in mouse presomitic mesoderm, paraxial mesoderm, and neural tube. Mature mouse protogenin is 1168 amino acids (aa) in length. It contains a 920 aa extracellular domain (ECD) (aa 24-943) plus a 224 aa cytoplasmic region. The ECD contains four consecutive Ig-like domains (aa 24-405), followed by five sequential fibronectin type III domains (aa 413-908). The ECD of mouse protogenin shares 98%, 87%, and 93% aa sequence identity with the ECD of rat, chick, and human protogenin, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Protogenin Antibody (BAF4919) (0)

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Product General Protocols

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Secondary Antibodies

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Effects of SDF-1-CXCR4/CXCR7 pathway on MSC chemotaxis in vitro.(A) The chemotaxis in response to SDF-1 alpha (10 ng/ml for 12 h) was performed in the NP-MSCs and HP-MSCs treated with a neutralizing anti-CXCR4 antibody, an anti-CXCR7 antibody, and the respective isotype-matched control antibodies. *P<0.05, vs NP-MSCs; †P<0.05, vs the respective isotype-matched control antibodies. (B) NP-MSCs were transiently overexpressed with CXCR4 using pORF9-mCXCR4 vector or with CXCR7 using pORF9-mCXCR7 vector (n = 6). A negative control empty (pORF9-MCS) vector was used. (C) The transfected cells were subjected to chemotaxis in response to the indicated concentrations of SDF-1 alpha for 12 h. *P<0.05, vs the empty vector. (D and E) Western blot analysis (D) and FCM (E) were performed to determine the intracellular and extracellular expression of both CXCR4 and CXCR7 in the cells treated with or without SDF-1 alpha (50 ng/ml for 60 min). (F) The chemotaxis in response to SDF-1 alpha was performed in the cells treated with or without SDF-1 alpha . Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22511954), licensed under a CC-BY license. Not internally tested by R&D Systems.

Sheep IgG Isotype Control [Unconjugated]
5-001-A

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Nestin Antibody (196908) [Unconjugated]
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Bioinformatics

Gene Symbol	PRTG
Uniprot	Q2EY15()

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Antibodies Secondary Antibodies Isotype Controls Proteins and Peptides ELISA Kits Sample Size New Products	About Us Careers Novus Guarantee Contact Us Our Team	Technical Support FAQs Help Finding Antibodies Protocols

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Protogenin Antibody [Biotin]

Species Glossary

Images

Product Details

Order Details

Protogenin Antibody [Biotin] Summary

Applications/Dilutions

Packaging, Storage & Formulations

Notes

Alternate Names for Protogenin Antibody [Biotin]

Background

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for Protogenin Antibody (BAF4919) (0)

Reviews for Protogenin Antibody (BAF4919) (0)

Product General Protocols

Video Protocols

FAQs for Protogenin Antibody (BAF4919) (0)

Secondary Antibodies

Isotype Controls

Additional Protogenin Products

Blogs on Protogenin

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Review this Product

Bioinformatics