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Protein Disulfide Isomerase/P4HB Antibody - BSA Free

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Western Blot: Protein Disulfide Isomerase/P4HB Antibody [NBP2-41319] - Analysis of PDIA1 in rat small intestine tissue lysate with PDIA1 antibody at (A) 1 and (B) 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody [NBP2-41319] - Immunofluorescence of Protein Disulfide Isomerase/P4HB in rat small intestine tissue with Protein Disulfide ...read more
Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP2-41319] - Rat small intestine tissue at 5 ug/ml.
Immunocytochemistry/ Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody - BSA Free [NBP2-41319] - Immunofluorescence of Protein Disulfide Isomerase/P4HB in rat small intestine tissue with Protein Disulfide ...read more
Immunocytochemistry/ Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody - BSA Free [NBP2-41319] - Immunofluorescence of Protein Disulfide Isomerase/P4HB in rat small intestine tissue with Protein Disulfide ...read more
Immunohistochemistry: Protein Disulfide Isomerase/P4HB Antibody - BSA Free [NBP2-41319] - Immunohistochemistry of Protein Disulfide Isomerase/P4HB in rat small intestine tissue with Protein Disulfide Isomerase/P4HB ...read more
Immunohistochemistry: Protein Disulfide Isomerase/P4HB Antibody - BSA Free [NBP2-41319] - Immunohistochemistry of Protein Disulfide Isomerase/P4HB in rat small intestine tissue with Protein Disulfide Isomerase/P4HB ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, RbSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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Protein Disulfide Isomerase/P4HB Antibody - BSA Free Summary

Immunogen
Antibody was raised against a 17 amino acid peptide near the center of human Protein Disulfide Isomerase/P4HB. The immunogen is located within amino acids 190 - 240 of Protein Disulfide Isomerase/P4HB. Amino Acid Squence: FKKFDEGRNNFEGEVTK
Predicted Species
Bovine (100%), Rabbit (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
P4HB
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1-2 ug/ml
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (88%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for Protein Disulfide Isomerase/P4HB Antibody - BSA Free

  • Cellular thyroid hormone-binding protein
  • collagen prolyl 4-hydroxylase beta
  • DSI
  • ERBA2L
  • GIT
  • glutathione-insulin transhydrogenase
  • P4HB
  • P4Hbeta
  • p55
  • PDI
  • PDIA1
  • PDIA1procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide (protein disulfide isomerase-associated 1)
  • PDIEC 5.3.4.1
  • PHDB
  • PO4DB
  • PO4HB
  • procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide
  • PROHB
  • PROHBprocollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide (protein disulfide isomerase; thyroid hormone binding protein p55)
  • Prolyl 4-hydroxylase subunit beta
  • prolyl 4-hydroxylase, beta polypeptide
  • protein disulfide isomerase family A, member 1
  • protein disulfide isomerase/oxidoreductase
  • protein disulfide isomerase-associated 1
  • protein disulfide-isomerase
  • protocollagen hydroxylase
  • Thbp
  • thyroid hormone-binding protein p55

Background

P4HB, the beta subunit of prolyl 4-hydroxylase, a highly abundant member of the PDI family. P4HB is a multifunctional enzyme, involved in hydroxylation of prolyl residues in preprocollagen, as well as continual rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol P4HB