Home » Protein Disulfide Isomerase/P4HB » Protein Disulfide Isomerase/P4HB Antibodies » Protein Disulfide Isomerase/P4HB Antibody (537331) [Unconjugated]
Novus Biologicals products are now on bio-techne.com

Protein Disulfide Isomerase/P4HB Antibody (537331) [Unconjugated]

 
 Submit a Review

Images

 
(
Enlarge)
Western blot shows lysates of PANC-1 human pancreatic carcinoma cell line and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Protein Disulfide Isomerase/P4HB Monoclonal Antibody (Catalog # MAB4236) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Protein Disulfide Isomerase/P4HB at approximately 57 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of PANC-1 human pancreatic carcinoma cell line and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Protein Disulfide Isomerase/P4HB Monoclonal ...read more
Protein Disulfide Isomerase/P4HB was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Protein Disulfide Isomerase/P4HB Monoclonal Antibody (Catalog # MAB4236) at 25 ...read more
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and human peripheral blood mononuclear cells (PBMC). PVDF Membrane was probed with 2 µg/mL of Human Protein Disulfide Isomerase/P4HB ...read more
Simple Western lane view shows lysates of PANC‑1 human pancreatic carcinoma cell line, loaded at 0.2 mg/ml. A specific band was detected for Protein Disulfide Isomerase/P4HB at approximately 59 kDa (as indicated) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IP, ICC/IF
Clone
537331
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB4236U
Alexa Fluor 405
FAB4236V
Alexa Fluor 488
IC4236G-100UG
Alexa Fluor 532
FAB4236X
Alexa Fluor 594
FAB4236T
Alexa Fluor 647
FAB4236R
Alexa Fluor 700
FAB4236N
Alexa Fluor 750
FAB4236S
Allophycocyanin
FAB4236A
Biotin
BAM4236
CoraFluor 1
FAB4236CL1
DyLight 350
FAB4236D
DyLight 405
FAB4236E
DyLight 488
FAB4236K
DyLight 550
FAB4236L
DyLight 594
FAB4236M
DyLight 650
FAB4236W
DyLight 680
FAB4236Y
DyLight 755
FAB4236Z
FITC
FAB4236F
HRP
FAB4236H
Janelia Fluor 525
FAB4236JF525
Janelia Fluor 549
FAB4236I
Janelia Fluor 585
FAB4236JF585
Janelia Fluor 635
FAB4236JF635
Janelia Fluor 646
FAB4236J
Janelia Fluor 669
FAB4236JF669
PE
FAB4236P
PerCP
FAB4236C
mFluor Violet 450 SE
FAB4236MFV450
mFluor Violet 500 SE
FAB4236MFV500
mFluor Violet 610 SE
FAB4236MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
IC4236G-100UG
Carrier Free
MAB4236-SP
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Protein Disulfide Isomerase/P4HB Antibody (537331) [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Protein Disulfide Isomerase/P4HB
Asp18-Lys505
Accession # P07237
Specificity
Detects human Protein Disulfide Isomerase/P4HB in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
P4HB
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1-2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Protein Disulfide Isomerase/P4HB Antibody (537331) [Unconjugated]

  • Cellular thyroid hormone-binding protein
  • collagen prolyl 4-hydroxylase beta
  • DSI
  • ERBA2L
  • GIT
  • glutathione-insulin transhydrogenase
  • P4HB
  • P4Hbeta
  • p55
  • PDI
  • PDIA1
  • PDIA1procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide (protein disulfide isomerase-associated 1)
  • PDIEC 5.3.4.1
  • PHDB
  • PO4DB
  • PO4HB
  • procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide
  • PROHB
  • PROHBprocollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide (protein disulfide isomerase; thyroid hormone binding protein p55)
  • Prolyl 4-hydroxylase subunit beta
  • prolyl 4-hydroxylase, beta polypeptide
  • protein disulfide isomerase family A, member 1
  • protein disulfide isomerase/oxidoreductase
  • protein disulfide isomerase-associated 1
  • protein disulfide-isomerase
  • protocollagen hydroxylase
  • Thbp
  • thyroid hormone-binding protein p55

Background

P4HB (Prolyl 4-hydroxylase beta chain; also PDI) is a 60 kDa member of the protein disulfide isomerase family.  As an intracellular homodimer, it forms a tetrameric complex with P4H alpha chains to form an active prolyl 4-hydrolyase.  This catalyses the hydroxylation of proline in collagen.  On the cell surface, it reduces disulfide bonds in HIV that allow the virus to fuse with CXCR4 and enter susceptible cells.  Mature human P4HB is 491 amino acids (aa) in length.  It contains two TRX domains (aa 25-134 and 368-475) plus an ER retention sequence (aa 505-508).  There is one potential isoform that shows an 11 aa substitution for the first 162 amino acids. Over aa 18-505, human P4HB shares 94% aa identity with mouse P4HB. 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(808 Publications)
Species: Hu
Applications: BA
IL-2 [Unconjugated]
202-IL
Bioactivity IL-2 [Unconjugated]
(4 Reviews)
(370 Publications)
Species: Hu
Applications: BA
TNF RII/TNFRSF1B
[HRP]
DRT200
N/A TNF RII/TNFRSF1B [HRP]
N/A TNF RII/TNFRSF1B [HRP]
N/A TNF RII/TNFRSF1B [HRP]
(75 Publications)
Species: Hu
Applications: ELISA
TXNDC15 Antibody
NBP1-89394
Immunocytochemistry/ Immunofluorescence TXNDC15 Antibody
Immunohistochemistry-Paraffin TXNDC15 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P
CDP/CUTL1 Antibody
(2A10)
H00001523-M01
Western Blot CDP/CUTL1 Antibody (2A10)
Immunohistochemistry-Paraffin CDP/CUTL1 Antibody (2A10)
ELISA CDP/CUTL1 Antibody (2A10)
(1 Review)
(4 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
IL-2 R beta
Antibody (27302)
MAB224
Immunohistochemistry IL-2 R beta Antibody (27302)
Neutralization IL-2 R beta Antibody (27302)
(7 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, IHC, Neut
LEDGF Antibody
(762815) [Unconjugate
MAB3468
Western Blot LEDGF Antibody (762815) [Unconjugated]
Immunocytochemistry LEDGF Antibody (762815) [Unconjugated]
(1 Review)
(1 Publication)
Species: Hu
Applications: ICC, WB
HCLS1 Antibody
(1A8)
H00003059-M02
Western Blot HCLS1 Antibody (1A8)
Proximity Ligation Assay HCLS1 Antibody (1A8)
Immunohistochemistry-Paraffin HCLS1 Antibody (1A8)
Species: Hu
Applications: ELISA, IHC, IHC-P, KD, PLA, S-ELISA, WB
KHSRP Antibody
NBP1-18910
Immunohistochemistry-Paraffin KHSRP Antibody
Immunohistochemistry-Paraffin KHSRP Antibody
Western Blot KHSRP Antibody
(1 Review)
(11 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, IP, Simple Western, WB
Siglec-7/CD328
Antibody [Unconjugate
AF1138
Neutralization Siglec-7/CD328 Antibody [Unconjugated]
(1 Review)
(4 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, IHC, Neut, WB
CTLA-4
[Unconjugated]
7268-CT
Bioactivity CTLA-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
HLA DQ/DR/DP
Antibody (HLA-Pan/296 ...
NBP2-79843
Protein Array HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Western Blot HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
NOD2 Antibody
(2D9) - BSA Free
NB100-524
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Immunohistochemistry-Frozen NOD2 Antibody (2D9) - BSA Free
(1 Review)
(26 Publications)
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(639 Publications)
Species: Mu
Applications: ELISA
TNF RI/TNFRSF1A
[HRP]
DRT100
N/A TNF RI/TNFRSF1A [HRP]
N/A TNF RI/TNFRSF1A [HRP]
N/A TNF RI/TNFRSF1A [HRP]
(84 Publications)
Species: Hu
Applications: ELISA
GRP78/HSPA5
Antibody - BSA Free
NBP1-06274
Flow Cytometry GRP78/HSPA5 Antibody - BSA Free
Flow Cytometry GRP78/HSPA5 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence GRP78/HSPA5 Antibody - BSA Free
(2 Reviews)
(43 Publications)
Species: Ce, Ch, Dr, Hu, Mu, Rt, Sh, Ze
Applications: Flow, ICC/IF, IHC-FrFl, IHC, IHC-P, Simple Western, WB
IL-4 [Unconjugated]
6507-IL/CF
Bioactivity IL-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
IL-10 [Biotin]
DY417
N/A IL-10 [Biotin]
(291 Publications)
Species: Mu
Applications: ELISA
Insulin Antibody
(182410) [Unconjugate ...
MAB1417
Immunocytochemistry Insulin Antibody (182410) [Unconjugated]
Immunohistochemistry Insulin Antibody (182410) [Unconjugated]
(24 Publications)
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
TPPP/p25 Antibody
NBP2-34031
Western Blot TPPP/p25 Antibody
Immunohistochemistry-Paraffin TPPP/p25 Antibody
Immunohistochemistry-Paraffin TPPP/p25 Antibody
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB

Publications for Protein Disulfide Isomerase/P4HB Antibody (MAB4236) (0)

There are no publications for Protein Disulfide Isomerase/P4HB Antibody (MAB4236).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Protein Disulfide Isomerase/P4HB Antibody (MAB4236) (0)

There are no reviews for Protein Disulfide Isomerase/P4HB Antibody (MAB4236). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Protein Disulfide Isomerase/P4HB Antibody (MAB4236) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Protein Disulfide Isomerase/P4HB Products

Blogs on Protein Disulfide Isomerase/P4HB

There are no specific blogs for Protein Disulfide Isomerase/P4HB, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Protein Disulfide Isomerase/P4HB Antibody (537331) [Unconjugated] and receive a gift card or discount.


Bioinformatics

Gene Symbol P4HB
Uniprot