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Protein Disulfide Isomerase/P4HB Antibody (537331) [Biotin]

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Protein Disulfide Isomerase/P4HB was detected in formaldehyde fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Protein Disulfide Isomerase/P4HB Biotinylated Monoclonal Antibody (Catalog # BAM4236) at 25 µg/mL overnight at 4 ° C. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (orange; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to the endoplasmic reticulum. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Protein Disulfide Isomerase/P4HB was detected in formaldehyde fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Protein Disulfide Isomerase/P4HB Biotinylated Monoclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF
Clone
537331
Clonality
Monoclonal
Host
Mouse
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB4236U
Alexa Fluor 405
FAB4236V
Alexa Fluor 488
IC4236G-100UG
Alexa Fluor 532
FAB4236X
Alexa Fluor 594
FAB4236T
Alexa Fluor 647
FAB4236R
Alexa Fluor 700
FAB4236N
Alexa Fluor 750
FAB4236S
Allophycocyanin
FAB4236A
CoraFluor 1
FAB4236CL1
DyLight 350
FAB4236D
DyLight 405
FAB4236E
DyLight 488
FAB4236K
DyLight 550
FAB4236L
DyLight 594
FAB4236M
DyLight 650
FAB4236W
DyLight 680
FAB4236Y
DyLight 755
FAB4236Z
FITC
FAB4236F
HRP
FAB4236H
Janelia Fluor 525
FAB4236JF525
Janelia Fluor 549
FAB4236I
Janelia Fluor 646
FAB4236J
Janelia Fluor 669
FAB4236JF669
PE
FAB4236P
PerCP
FAB4236C
Unconjugated
MAB4236
mFluor Violet 450 SE
FAB4236MFV450
mFluor Violet 500 SE
FAB4236MFV500
mFluor Violet 610 SE
FAB4236MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB4236
Carrier Free
IC4236G-100UG
Carrier Free
MAB4236
Carrier Free
MAB4236-SP
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Protein Disulfide Isomerase/P4HB Antibody (537331) [Biotin] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Protein Disulfide Isomerase/P4HB
Asp18-Lys505
Accession # P07237
Specificity
Conjugated Protein Disulfide Isomerase/P4HB antibodies are ideal for immunocytochemistry colocalization studies in the lumen of the endoplasmic reticulum. The unconjugated antibody detects human Protein Disulfide Isomerase/P4HB in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
P4HB
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
Readout System

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Protein Disulfide Isomerase/P4HB Antibody (537331) [Biotin]

  • Cellular thyroid hormone-binding protein
  • collagen prolyl 4-hydroxylase beta
  • DSI
  • ERBA2L
  • GIT
  • glutathione-insulin transhydrogenase
  • P4HB
  • P4Hbeta
  • p55
  • PDI
  • PDIA1
  • PDIA1procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide (protein disulfide isomerase-associated 1)
  • PDIEC 5.3.4.1
  • PHDB
  • PO4DB
  • PO4HB
  • procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide
  • PROHB
  • PROHBprocollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), betapolypeptide (protein disulfide isomerase; thyroid hormone binding protein p55)
  • Prolyl 4-hydroxylase subunit beta
  • prolyl 4-hydroxylase, beta polypeptide
  • protein disulfide isomerase family A, member 1
  • protein disulfide isomerase/oxidoreductase
  • protein disulfide isomerase-associated 1
  • protein disulfide-isomerase
  • protocollagen hydroxylase
  • Thbp
  • thyroid hormone-binding protein p55

Background

P4HB (Prolyl 4-hydroxylase beta chain; also PDI) is a 60 kDa member of the protein disulfide isomerase family.  As an intracellular homodimer, it forms a tetrameric complex with P4H alpha chains to form an active prolyl 4-hydrolyase.  This catalyses the hydroxylation of proline in collagen.  On the cell surface, it reduces disulfide bonds in HIV that allow the virus to fuse with CXCR4 and enter susceptible cells.  Mature human P4HB is 491 amino acids (aa) in length.  It contains two TRX domains (aa 25-134 and 368-475) plus an ER retention sequence (aa 505-508).  There is one potential isoform that shows an 11 aa substitution for the first 162 amino acids. Over aa 18-505, human P4HB shares 94% aa identity with mouse P4HB. 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol P4HB
Uniprot