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Recombinant Human Proteasome 20S beta 3 His Protein

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SDS-Page: Recombinant Human Proteasome 20S beta 3 His Protein [NBP1-72544] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
SDS-Page: Recombinant Human Proteasome 20S beta 3 His Protein [NBP1-72544] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.25 mg/ml

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Recombinant Human Proteasome 20S beta 3 His Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-205 of Human Proteasome 20S beta 3

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSIMSYNGGA VMAMKGKNCV AIAADRRFGI QAQMVTTDFQ KIFPMGDRLY IGLAGLATDV QTVAQRLKFR LNLYELKEGR QIKPYTLMSM VANLLYEKRF GPYYTEPVIA GLDPKTFKPF ICSLDLIGCP MVTDDFVVSG TCAEQMYGMC ESLWEPNMDP DHLFETISQA MLNAVDRDAV SGMGVIVHII EKDKITTRTL KARMD
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
PSMB3
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
25.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH 8.0), 50% glycerol, 0.2M NaCl
Preservative
No Preservative
Concentration
0.25 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human Proteasome 20S beta 3 His Protein

  • EC 3.4.25.1
  • HC10-II
  • MGC4147
  • proteasome (prosome, macropain) subunit, beta type, 3
  • Proteasome 20S beta 3
  • Proteasome chain 13
  • Proteasome component C10-II
  • proteasome subunit beta type-3
  • Proteasome theta chain
  • PSMB3

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides.. Recombinant human PSMB3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol PSMB3