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Proteasome 20S alpha 5 Antibody

Images

 
Western Blot: Proteasome 20S alpha 5 Antibody [NBP1-32387] - Proteasome 20S alpha 5 antibody detects Proteasome 20S alpha 5 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 12% ...read more
Immunocytochemistry/ Immunofluorescence: Proteasome 20S alpha 5 Antibody [NBP1-32387] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Proteasome 20S alpha 5 Antibody [NBP1-32387] - Proteasome 20S alpha 5 antibody detects Proteasome 20S alpha 5 protein at cytoplasm and nucleus in human hepatocellular carcinoma by ...read more
Western Blot: Proteasome 20S alpha 5 Antibody [NBP1-32387] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE; antibody diluted at 1:5000.
Western Blot: Proteasome 20S alpha 5 Antibody [NBP1-32387] - Sample(30 ug whole cell lysate)A: HeLa S3 12% SDS PAGE, antibody diluted at 1:500.
Immunohistochemistry-Paraffin: Proteasome 20S alpha 5 Antibody [NBP1-32387] - Paraffin-embedded H1299 xenograft, using antibody at 1:100 dilution.

Product Details

Summary
Reactivity Hu, Mu, RMSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Proteasome 20S alpha 5 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Proteasome 20S alpha 5. The exact sequence is proprietary.
Localization
Cytoplasm, Nucleus
Predicted Species
Rhesus Macaque (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PSMA5
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:10000

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for Proteasome 20S alpha 5 Antibody

  • EC 3.4.25.1
  • FLJ42315
  • macropain subunit zeta
  • Macropain zeta chain
  • MGC117302
  • MGC125802
  • MGC125803
  • MGC125804
  • Multicatalytic endopeptidase complex zeta chain
  • proteasome (prosome, macropain) subunit, alpha type, 5
  • Proteasome 20S alpha 5
  • proteasome alpha 5 subunit
  • proteasome component 5
  • proteasome subunit alpha type-5
  • proteasome subunit zeta
  • Proteasome zeta chain
  • PSC5
  • PSMA5
  • ZETA

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PSMA5