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PSMB5 Antibody

Images

 
Western Blot: PSMB5 Antibody [NBP2-13820] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Hep-G2
Immunocytochemistry/ Immunofluorescence: PSMB5 Antibody [NBP2-13820] - Staining of human cell line PC-3 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PSMB5 Antibody [NBP2-13820] - Staining of human cerebellum shows strong nuclear positivity in cells in granular layer.
Immunohistochemistry-Paraffin: PSMB5 Antibody [NBP2-13820] - Staining of human testis shows strong nuclear positivity in a subset of cells in seminiferous ducts.
Immunohistochemistry-Paraffin: PSMB5 Antibody [NBP2-13820] - Staining of human kidney shows strong nuclear positivity in cells in glomeruli.
Immunohistochemistry-Paraffin: PSMB5 Antibody [NBP2-13820] - Staining of human skin shows no positivity in squamous epithelial cells as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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PSMB5 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: YRDAYSGGAVNLYHVREDGWIRVSSDNVADLHEKYSGST
Predicted Species
Mouse (90%), Rat (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PSMB5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
PSMB5 Protein (NBP2-13820PEP)
Publications
Read Publication using NBP2-13820.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PSMB5 Antibody

  • LMPX
  • LMPXproteasome beta 5 subunit
  • Macropain epsilon chain
  • MB1
  • MB1EC 3.4.25.1
  • Multicatalytic endopeptidase complex epsilon chain
  • proteasome (prosome, macropain) subunit, beta type, 5
  • proteasome catalytic subunit 3
  • Proteasome chain 6
  • Proteasome epsilon chain
  • proteasome subunit beta type-5
  • Proteasome subunit MB1
  • Proteasome subunit X
  • proteasome subunit, beta type, 5
  • PSMB5
  • PSX large multifunctional protease X
  • XMGC104214

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PSMB5 Antibody (NBP2-13820)(1)

We have publications tested in 1 application: IHC-Fr.


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IHC-Fr
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PSMB5 Antibody (NBP2-13820) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for PSMB5 Antibody (NBP2-13820)

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Blogs on PSMB5

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Bioinformatics

Gene Symbol PSMB5