UCH-L1/PGP9.5 Antibody (31A3)

UchL1 (ubiquitin C-terminal hydrolase), also known as PGP9.5 (protein gene product 9.5) and PARK5, is a neuronal biomarker and ubquitin system protein (reviewed in Day and Thompson, 2010). UchL1 is a highly conserved antibody and has shown that it is expressed in neurons and neuroendocrine cells in vertebrates where it comprises about 5-10% of soluble cytoplasmic proteins. A minor proportion of UchL1 in brain is tightly bound to the membrane. UchL1 antibody is also expressed in human oocytes and spermatogonia. It is important to note that the use of an antibody as a biomarker in this case does not imply that an antibody defines absolute tissue specificity. Rather, it means that PGP9.5 is expressed in neurons and neuroendrocine cells at significantly higher levels than in other cell types.
Functionally, UchL1 antibody is a thiol protease enzyme that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. UchL1 antibody also binds to free monoubiquitin and inhibits monoubiquitin degradation in lysosomes; it may function to stabilize monoubiquitin within neurons. A mutation in the UchL1 gene has been found to cause a form of Parkinson's disease, and this discovery has spurred considerable research interest in UchL1 and to its alternative name of PARK5.
Antibody to UchL1 is widely used as an immunohistochemical marker of nerves and neuroendocrine cells. The UchL1 clone 31A3 antibody stains neuronal cell bodies and axons in the central and peripheral nervous systems as well as small nerve fibers in peripheral tissues, including epidermal tissues (Day & Thompson, 2010). The antibody also stains neuroendrocrine cells in the kidney, pituitary, thyroid, pancreas, gastrointestinal tract, and tumors of the diffuse neuroendocrine system. This antibody has also identified UchL1 expression in renal tubule spermatogonia, testis, ovary, and both pregnant and non-pregnant corpus luteum. In western blots, the antibody identifies UchL1 as a band of approximately 20-30 kDa. The antibody is specific to UchL1 and does not cross-react with the closely related UchL3 protein (Yi et al, 2007).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Zebrafish.

We have publications tested in 5 applications: ICC/IF, IF/IHC, IHC-Fr, IHC-P, WB.

Showing Publications 1 - 10 of 18. Show All 18 Publications. Collapse Publications.

Publications using NB600-1160	Applications	Species
Shi WG, Yao Y, Liang YJ et Al. Activation of TGR5 in the injured nerve site according to a prevention protocol mitigates partial sciatic nerve ligation-induced neuropathic pain by alleviating neuroinflammation Pain 2024-10-25 [PMID: 39450924]
Duan J, Lv A, Guo Z et al. CX3CR1 + /UCHL1 + microglial extracellular vesicles in blood: a potential biomarker for multiple sclerosis Journal of Neuroinflammation 2024-10-09 [PMID: 39385200]
Kim CY, Lee EH, Kwak SH et al. UCHL1 Overexpression Is Related to the Aggressive Phenotype of Non-small Cell Lung Cancer Tuberculosis and Respiratory Diseases 2024-10-01 [PMID: 39362830]
Lucarini E, Micheli L, Pagnotta E et al. The Efficacy of Camelina sativa Defatted Seed Meal against Colitis-Induced Persistent Visceral Hypersensitivity: The Relevance of PPAR alpha Receptor Activation in Pain Relief Nutrients 2022-07-29 [PMID: 35956313] (IHC-P, Rat) Details: Dilution used 1:500	IHC-P	Rat
Manavis J, Blumbergs P, Jerrett I et al. Heterogeneous immunoreactivity of axonal spheroids in focal symmetrical encephalomalacia produced by Clostridium perfringens type D epsilon toxin in sheep Veterinary pathology 2021-12-06 [PMID: 34872408]
Pal P, Hales K, Hales DB Increased Transient Receptor Potential Melastatin 8 Expression in the development of bladder pain in patients with Interstitial cystitis/Bladder Pain Syndrome Oncotarget 2020-10-10 [PMID: 33088425] (ICC/IF, Human)	ICC/IF	Human
Moutal A, Ji Y, Bellampalli SS, Khanna R Differential expression of Cdk5-phosphorylated CRMP2 following a spared nerve injury Mol Brain 2020-06-22 [PMID: 32571373] (IF/IHC, Rat)	IF/IHC	Rat
Arnaboldi F, Sommariva M, Opizzi E et al. Expression of Toll-like receptors 4 and 7 in murine peripheral nervous system development Ann. Anat. 2020-05-04 [PMID: 32380196] (ICC/IF, Mouse)	ICC/IF	Mouse
Alberio L, Locarno A, Saponaro A et al. A light-gated potassium channel for sustained neuronal inhibition. Nat. Methods. 2018-11-01 [PMID: 30377377] (IF/IHC, Zebrafish)	IF/IHC	Zebrafish
Sundaresan S, Meininger C A et al. Gastrin Induces Nuclear Export and Proteasome Degradation of Menin in Enteric Glial Cells. Gastroenterology 2017-01-12 [PMID: 28859856] (IF/IHC, ICC/IF, Mouse)	IF/IHC, ICC/IF	Mouse
Novakovic P, Harding JC, Al-Dissi AN et al. Pathologic Evaluation of Type 2 Porcine Reproductive and Respiratory Syndrome Virus Infection at the Maternal-Fetal Interface of Late Gestation Pregnant Gilts PLoS ONE 2016-03-11 [PMID: 26963101] (IF/IHC, WB, Human)	IF/IHC, WB	Human
Read NC, Gutsol A, Holterman CE et al. Ubiquitin C-terminal hydrolase L1 deletion ameliorates glomerular injury in mice with ACTN4-associated focal segmental glomerulosclerosis. Biochim. Biophys. Acta 2014-03-22 [PMID: 24662305] (IHC-P, Mouse)	IHC-P	Mouse
Bottner M, Zorenkov D, Hellwig I et al. Expression pattern and localization of alpha-synuclein in the human enteric nervous system. Neurobiol Dis. 2012-12-01 [PMID: 22850485] (IHC-Fr) Details: IHC (frozen); Table I (colon carcinoma, dysplastic adenoma).	IHC-Fr
Sugimoto K, Kojima K, Baba M, Yasujima M. Olmesartan ameliorates peripheral nerve dysfunction in Zucker diabetic fatty rats. J Hypertens. 2011-07-01 [PMID: 21602711] (IHC-Fr, Rat) Details: IHC (frozen); Fig 3 (rat skin).	IHC-Fr	Rat
Day IN, Thompson RJ. UCHL1 (PGP 9.5): neuronal biomarker and ubiquitin system protein. Prog Neurobiol. 2010-03-01 [PMID: 19879917] (IHC-P) Details: IHC (paraffin): Fig 2a,b (human brain). The distribution of PGP9.5 is extensively described in this review article, researchers are encouraged to consult this outstanding article for additional information.	IHC-P
Gaunitz C, Gabert J, Lucker E et al. Suitability of antigens PGP 9.5 and neurofilament light as marker proteins for detection of neuronal tissue in processed meat products. J Food Prot. 2009-05-01 [PMID: 19517736] Details: Sandwich ELISA (cow and pig meat). PGP9.5 antibodies from clones 13C4 and 31A3 were used to detect neuronal contaminant components in meat. Refer to the publication for additional details.
Yi YJ, Manandhar G, Sutovsky M et al. Ubiquitin C-terminal hydrolase-activity is involved in sperm acrosomal function and anti-polyspermy defense during porcine fertilization. Biol Reprod. 2007-11-01 [PMID: 17671268] (WB, Mouse) Details: WB: Fig 6a (mouse testicular cells and spermatozoa, boar testicular cells). UchL1 was detected just between the 19 and 26 kDa markers. Note: UchL1 was not detected in boar testicular cells by WB.	WB	Mouse
Stander S, Schmelz M, Metze D et al. Distribution of cannabinoid receptor 1 (CB1) and 2 (CB2) on sensory nerve fibers and adnexal structures in human skin. J Dermatol Sci. 2005-06-01 [PMID: 15927811] (IHC-Fr, Human) Details: IHC (frozen); Fig 2E (human skin).	IHC-Fr	Human
Show All 18 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	ICC	Rat	03/26/2015	View Summary Application Immunocytochemistry Sample Tested Mesenteric artery-whole Species Rat Lot 7306-1p140206

Array NB600-1160

• UCH-L1/PGP9.5 Antibodies
• UCH-L1/PGP9.5 Lysate
• UCH-L1/PGP9.5 Proteins
• UCH-L1/PGP9.5 ELISA
• UCH-L1/PGP9.5 Proteins and Enzyme