Polypeptide GalNac Transferase 3/GALNT3 Antibody

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

O-glycosylation is a ubiquitous post-translational modification present in secreted and membrane‑bound proteins. Polypeptide N‑acetylgalactosaminyltransferases (GALNTs) calalyze the initial step for O-glycosylation by transferring GalNAc to Thr or Ser residues (GalNAc alpha 1-O-Ser/Thr) in the Golgi compartment. Structurally, the GALNTs consist of an N-terminal catalytic domain tethered by a short linker to a C-terminal ricin-like lectin domain containing three potential carbohydrate-binding sites (1, 2). Twenty distinct GALNT isoforms have been detected in humans. These isoforms display both unique and overlapping substrate specificities (3, 4, 5) with no known universal consensus glycosylation sequence. Glycosylation of mucins results from the successive, often hierarchical, action of several specific GALNTs (6). Expression of GALNT3 appears to be highly regulated and mainly found in pancreas and testis (7). Using a peptide library screening approach, GALNT3 was classified as an intermediate transferase that increases the density of O-linked glycans within the mucin domain following glycosylation with early transferases (5). The enzymatic activity of recombinant human GALNT3 was determined using a phosphatase‑coupled assay (8).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Mass Spectrometry, Western Blot.

Showing Publications 1 - 3 of 3.

Publications using AF7174	Applications	Species
Tachida, Y;Iijima, J;Takahashi, K;Suzuki, H;Kizuka, Y;Yamaguchi, Y;Tanaka, K;Nakano, M;Takakura, D;Kawasaki, N;Saito, Y;Manya, H;Endo, T;Kitazume, S; O-GalNAc glycosylation determines intracellular trafficking of APP and A? production The Journal of biological chemistry 2023-06-09 [PMID: 37302553] (Mass Spectrometry, Human)	Mass Spectrometry	Human
Rodriguez E, Boelaars K, Brown K et al. Analysis of the glyco-code in pancreatic ductal adenocarcinoma identifies glycan-mediated immune regulatory circuits Communications biology 1/11/2022 [PMID: 35017635]
Y Chu, A Kilikevici, J Liu, KC Johnson, S Yokota, DR Corey Argonaute binding within 3'-untranslated regions poorly predicts gene repression Nucleic Acids Res., 2020-07-27;0(0):. 2020-07-27 [PMID: 32501500] (Western Blot, Human)	Western Blot	Human

Array AF7174

• Polypeptide GalNac Transferase 3/GALNT3 Antibodies
• Polypeptide GalNac Transferase 3/GALNT3 Lysate
• Polypeptide GalNac Transferase 3/GALNT3 Protein
• Polypeptide GalNac Transferase 3/GALNT3 Proteins and Enzyme