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GALNT6 Antibody

Images

 
Western Blot: GALNT6 Antibody [NBP1-86059] - Analysis in control (vector only transfected HEK293T lysate) and GALNT6 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T ...read more
Immunocytochemistry/ Immunofluorescence: GALNT6 Antibody [NBP1-86059] - Staining of human cell line MCF7 shows localization to nucleoplasm & the Golgi apparatus. Antibody staining is shown in green.
Orthogonal Strategies: Immunohistochemistry-Paraffin: GALNT6 Antibody [NBP1-86059] - Staining in human stomach and liver tissues using anti-GALNT6 antibody. Corresponding GALNT6 RNA-seq data are presented for the ...read more
Immunohistochemistry-Paraffin: GALNT6 Antibody [NBP1-86059] - Staining of human liver shows low expression as expected.
Immunohistochemistry-Paraffin: GALNT6 Antibody [NBP1-86059] - Staining of human stomach shows high expression.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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GALNT6 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: PWLKSLVSRKDHVLDLMLEAMNNLRDSMPKLQIRAPEAQQTLFSINQSCLPGFYTPAELKPFWERPPQDPNAPGADGKAFQKSKWTPLETQEKEE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GALNT6
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
GALNT6 Protein (NBP1-86059PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (80%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for GALNT6 Antibody

  • EC 2.4.1.41
  • GalNAc transferase 6
  • GalNAcT6
  • GALNAC-T6
  • Polypeptide GalNAc transferase 6
  • polypeptide N-acetylgalactosaminyltransferase 6
  • pp-GaNTase 6
  • Protein-UDP acetylgalactosaminyltransferase 6
  • UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 6
  • UDP-N-acetyl-alpha-D-galactosamine: polypeptideN-acetylgalactosaminyltransferase 6
  • UDP-N-acetyl-alpha-D-galactosamine:polypeptideN-acetylgalactosaminyltransferase 6 (GalNAc-T6)

Background

GALNT6 encodes a member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes. GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi apparatus by catalyzing the transfer of GalNAc to serine and threonine residues on target proteins. They are characterized by an N-terminal transmembrane domain, a stem region, a lumenal catalytic domain containing a GT1 motif and Gal/GalNAc transferase motif, and a C-terminal ricin/lectin-like domain. GalNAc-Ts have different, but overlapping, substrate specificities and patterns of expression. The encoded protein is capable of glycosylating fibronectin peptide in vitro and is expressed in a fibroblast cell line, indicating that it may be involved in the synthesis of oncofetal fibronectin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol GALNT6