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B4GALNT1 Antibody

Images

 
Independent Antibodies: Immunohistochemistry-Paraffin: B4GALNT1 Antibody [NBP1-88662] - Staining of human cerebellum, cerebral cortex, lymph node and pancreas using Anti-B4GALNT1 antibody NBP1-88662 (A) shows ...read more
Western Blot: B4GALNT1 Antibody [NBP1-88662] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: B4GALNT1 Antibody [NBP1-88662] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Western Blot: B4GALNT1 Antibody [NBP1-88662] - Analysis in human cell line A-549.
Immunohistochemistry-Paraffin: B4GALNT1 Antibody [NBP1-88662] - Staining of human cerebellum shows strong granular cytoplasmic positivity in Purkinje cells.
Immunohistochemistry-Paraffin: B4GALNT1 Antibody [NBP1-88662] - Staining of human cerebral cortex shows strong granular cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: B4GALNT1 Antibody [NBP1-88662] - Staining of human lymph node shows no positivity in germinal center cells as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

       

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B4GALNT1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LAPEPRYAHIPVRIKEQVVGLLAWNNCSCESSGGGLPLPFQKQVRAIDLTKAFDPAELRAASATREQEFQAFLSRSQSPADQLLIAPANSPLQYPLQGVEVQPLRSILVPGLSLQAASGQE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
B4GALNT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
B4GALNT1 Protein (NBP1-88662PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for B4GALNT1 Antibody

  • (N-acetylneuraminyl)-galactosylglucosylceramide
  • B4GALNT1
  • beta-1,4 N-acetylgalactosaminyltransferase 1
  • beta1,4GalNAc-T
  • beta-1,4-N-acetyl-galactosaminyl transferase 1
  • beta1-4GalNAc-T
  • GALGT
  • GALGTEC 2.4.1.92
  • GALNACT
  • GalNAc-T
  • GD2 Synthase
  • GD2 synthase, GM2 synthase
  • GM2 Synthase
  • GM2/GD2 synthase
  • SIAT2
  • SPG26
  • UDP-Gal:betaGlcNAc beta-1,4-N-acetylgalactosaminyltransferase transferase 1
  • UDP-N-acetyl-alpha-D-galactosamine:(N-acetylneuraminyl)-galactosylglucosylceramideN-acetylgalactosaminyltransferase (GalNAc-T)

Background

GM2 and GD2 gangliosides are sialic acid-containing glycosphingolipids. GalNAc-T is the enzyme involved in the biosynthesis of G(M2) and G(D2) glycosphingolipids. GalNAc-T catalyzes the transfer of GalNAc into G(M3) and G(D3) by a beta-1,4 linkage, resulting in the synthesis of G(M2) and G(D2), respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol B4GALNT1